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4-nitrophenyl alpha-D-galactopyranoside + acetyl-CoA
4-nitrophenyl 6-O-acetyl-alpha-D-galactopyranoside + CoA
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + acetyl-CoA
4-nitrophenyl 6-O-acetyl-beta-D-galactopyranoside + CoA
-
-
-
-
?
acetyl-CoA + a beta-D-galactoside
CoA + a 6-acetyl-beta-D-galactoside
acetyl-CoA + beta-D-galactoside
CoA + 6-acetyl-beta-D-galactoside
-
-
-
-
?
acetyl-CoA + beta-D-methylgalactoside
CoA + 6-O-acetyl-beta-D-methylgalactoside
-
-
-
-
?
acetyl-CoA + beta-D-phenylgalactoside
CoA + 6-O-acetyl-beta-D-phenylgalactoside
-
-
-
-
?
acetyl-CoA + beta-D-phenylglucoside
CoA + 6-O-acetyl-beta-D-phenylglucoside
-
-
-
-
?
acetyl-CoA + beta-D-thiodigalactoside
CoA + 6-O-acetyl-beta-D-thiodigalactoside
-
-
-
-
?
acetyl-CoA + beta-D-thiophenylglucoside
CoA + 6-O-acetyl-beta-D-thiophenylglucoside
-
-
-
-
?
acetyl-CoA + butyl beta-D-thiogalactoside
CoA + butyl 6-O-acetyl-beta-D-thiogalactoside
-
-
-
-
?
acetyl-CoA + isopropyl beta-D-thiogalactoside
CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
acetyl-CoA + methyl beta-D-thiogalactoside
CoA + methyl 6-O-acetyl-beta-D-thiogalactoside
acetyl-CoA + o-nitrophenyl-beta-D-galactopyranoside
CoA + o-nitrophenyl 6-O-acetyl-beta-D-galactoside
-
-
-
-
?
acetyl-CoA + o-nitrophenyl-beta-D-galactoside
CoA + o-nitrophenyl 6-O-acetyl-beta-D-galactoside
-
-
-
-
?
acetyl-CoA + o-nitrophenyl-beta-D-thiogalactoside
CoA + o-nitrophenyl 6-O-acetyl-beta-D-thiogalactoside
-
-
-
-
?
acetyl-CoA + p-nitrophenyl-beta-D-galactopyranoside
CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactopyranoside
-
-
-
-
?
acetyl-CoA + p-nitrophenyl-beta-D-galactoside
CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactoside
acetyl-CoA + p-nitrophenyl-beta-D-glucopyranoside
CoA + p-nitrophenyl 6-O-acetyl-beta-D-glucopyranoside
-
-
-
-
?
acetyl-CoA + p-nitrophenyl-beta-D-glucoside
CoA + p-nitrophenyl-6-O-acetyl-beta-D-glucoside
acetyl-CoA + p-nitrophenyl-beta-D-lactopyranoside
CoA + p-nitrophenyl 6-O-acetyl-beta-D-lactopyranoside
-
-
-
-
?
acetyl-CoA + p-nitrophenyl-beta-D-lactoside
CoA + p-nitrophenyl 6-O-acetyl-beta-D-lactoside
-
-
-
-
?
acetyl-CoA + p-nitrophenyl-beta-D-thiogalactoside
CoA + p-nitrophenyl 6-O-acetyl-beta-D-thiogalactoside
-
-
-
-
?
acetyl-CoA + phenyl beta-D-thiogalactoside
CoA + 6-O-acetyl-beta-D-thiophenylgalactoside
-
-
-
-
?
butyryl-CoA + p-nitrophenyl-beta-D-galactopyranoside
CoA + p-nitrophenyl 6-O-butyryl-beta-D-galactopyranoside
-
-
-
-
?
isopropyl-thio-beta-D-galactopyranoside
?
-
-
-
-
?
isopropyl-thio-beta-D-galactopyranoside + acetyl-CoA
6-acetyl-isopropyl-thio-beta-D-galactopyranoside + CoA
-
-
-
-
?
isopropyl-thio-beta-D-galactopyranoside + acetyl-CoA
isopropyl 6-O-acetyl-1-thio-beta-D-galactopyranoside + CoA
-
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside
?
-
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside + acetyl-CoA
p-nitrophenyl 6-O-acetyl-beta-D-galactopyranoside + CoA
-
-
-
-
?
propionyl-CoA + p-nitrophenyl-beta-D-galactopyranoside
CoA + p-nitrophenyl 6-O-propionyl-beta-D-galactopyranoside
-
-
-
-
?
additional information
?
-
acetyl-CoA + a beta-D-galactoside
CoA + a 6-acetyl-beta-D-galactoside
-
-
-
-
?
acetyl-CoA + a beta-D-galactoside
CoA + a 6-acetyl-beta-D-galactoside
-
prevents accumulation of methyl-1-thio-beta-D-galactoside, serves as a backup device to avoid metabolic congestion
-
-
?
acetyl-CoA + a beta-D-galactoside
CoA + a 6-acetyl-beta-D-galactoside
galactoside acetyltransferases are enzymes that transfer an acetyl group from acetyl coen-zyme A to beta-galactosides. The enzymes have a broad substrate specificity and can acetylate many galactoside derivatives, including thiogalactosides and lactosides
-
-
?
acetyl-CoA + a beta-D-galactoside
CoA + a 6-acetyl-beta-D-galactoside
CoA binds in the active site formed by the residues from adjacent LbetaH domains. After determination of CoA-bound structure, molecular dynamics simulations are performed to model the binding of AcCoA. Binding of both AcCoA and CoA to SACOL2570 is verified by isothermal titration calorimetry
binding of CoA in the 3V4E crystal structure involves residues from two adjacent monomers in the homotrimeric assembly. Residues responsible for CoA binding by SACOL2570 are Asn84, Ala112, Gly141, Lys165, and Arg182
-
?
acetyl-CoA + isopropyl beta-D-thiogalactoside
CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
-
-
-
?
acetyl-CoA + isopropyl beta-D-thiogalactoside
CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
-
-
?
acetyl-CoA + isopropyl beta-D-thiogalactoside
CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
-
-
-
?
acetyl-CoA + isopropyl beta-D-thiogalactoside
CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
-
-
?
acetyl-CoA + isopropyl beta-D-thiogalactoside
CoA + isopropyl 6-O-acetyl-beta-D-thiogalactoside
-
-
-
-
?
acetyl-CoA + methyl beta-D-thiogalactoside
CoA + methyl 6-O-acetyl-beta-D-thiogalactoside
-
-
-
-
?
acetyl-CoA + methyl beta-D-thiogalactoside
CoA + methyl 6-O-acetyl-beta-D-thiogalactoside
-
-
-
-
?
acetyl-CoA + p-nitrophenyl-beta-D-galactoside
CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactoside
-
twice as effective as isopropyl-beta-D-thiogalactoside
-
-
?
acetyl-CoA + p-nitrophenyl-beta-D-galactoside
CoA + p-nitrophenyl 6-O-acetyl-beta-D-galactoside
-
twice as effective as isopropyl-beta-D-thiogalactoside
-
-
?
acetyl-CoA + p-nitrophenyl-beta-D-glucoside
CoA + p-nitrophenyl-6-O-acetyl-beta-D-glucoside
-
-
-
-
?
acetyl-CoA + p-nitrophenyl-beta-D-glucoside
CoA + p-nitrophenyl-6-O-acetyl-beta-D-glucoside
-
-
-
-
?
additional information
?
-
-
palmitoyl-CoA
-
-
?
additional information
?
-
-
inactive: succinyl-CoA
-
-
?
additional information
?
-
-
crotonyl-CoA
-
-
?
additional information
?
-
-
oleoyl-CoA
-
-
?
additional information
?
-
-
enzyme catalyses transfer of an acetyl group from acetyl-CoA to the 6-hydroxyl group of a beta-D-galactoside
-
-
?
additional information
?
-
-
enzyme catalyses transfer of an acetyl group from acetyl-CoA to the 6-hydroxyl group of a beta-D-galactoside
-
-
?
additional information
?
-
-
butyryl-CoA is about 10% as effective as acetyl-CoA, enzyme catalyzes the transfer of acetyl from acetyl-CoA to thiogalactosides but not or at much slower rate to galactosides except when a phenyl group is attached to the oxygen atom at carbon 1, a phenyl group substitution also allows glucosides to react
-
-
?
additional information
?
-
-
it is suggested that the enzyme plays an important role in lactose utilization
-
-
?
additional information
?
-
-
crotonyl-CoA
-
-
?
additional information
?
-
-
oleoyl-CoA
-
-
?
additional information
?
-
-
palmitoyl-CoA
-
-
?
additional information
?
-
-
inactive: succinyl-CoA
-
-
?
additional information
?
-
-
butyryl-CoA is about 10% as effective as acetyl-CoA, enzyme catalyzes the transfer of acetyl from acetyl-CoA to thiogalactosides but not or at much slower rate to galactosides except when a phenyl group is attached to the oxygen atom at carbon 1, a phenyl group substitution also allows glucosides to react
-
-
?
additional information
?
-
-
it is suggested that the enzyme plays an important role in lactose utilization
-
-
?
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24.1 - 97
4-nitrophenyl-beta-D-galactopyranoside
0.221
butyryl-CoA
-
p-nitrophenyl-beta-D-galactopyranoside
360 - 1500
isopropyl beta-D-thiogalactoside
629 - 2720
isopropyl-thio-beta-D-galactopyranoside
67.9
o-nitrophenyl-beta-D-galactopyranoside
-
acetyl donor: acetyl-CoA
57.9
p-nitrophenyl-alpha-D-galactopyranoside
-
acetyl donor: acetyl-CoA
12 - 97
p-nitrophenyl-beta-D-galactopyranoside
63.4
phenyl-beta-D-galactopyranoside
-
acetyl donor: acetyl-CoA
0.098
propionyl-CoA
-
p-nitrophenyl-beta-D-galactopyranoside
24.1
4-nitrophenyl-beta-D-galactopyranoside
-
25°C, mutant Y83L
27.2
4-nitrophenyl-beta-D-galactopyranoside
-
25°C, wild-type enzyme
29.5
4-nitrophenyl-beta-D-galactopyranoside
-
25°C, mutant Y83F
97
4-nitrophenyl-beta-D-galactopyranoside
-
25°C, mutant Y83V
0.04
acetyl-CoA
-
-
0.045
acetyl-CoA
-
E. coli K12
0.049
acetyl-CoA
-
E. coli ML308
0.059
acetyl-CoA
-
phenyl-beta-D-galactopyranoside
0.065
acetyl-CoA
-
p-nitrophenyl-beta-D-galactopyranoside
0.082
acetyl-CoA
-
o-nitrophenyl-beta-D-galactopyranoside
0.104
acetyl-CoA
-
p-nitrophenyl-beta-D-galactopyranoside
0.266
acetyl-CoA
-
p-nitrophenyl-beta-D-lactopyranoside
360
isopropyl beta-D-thiogalactoside
-
-
770
isopropyl beta-D-thiogalactoside
-
-
1200
isopropyl beta-D-thiogalactoside
-
E. coli K12
1500
isopropyl beta-D-thiogalactoside
-
E. coli ML308
629
isopropyl-thio-beta-D-galactopyranoside
-
hexahistidine-tagged wild type enzyme
770
isopropyl-thio-beta-D-galactopyranoside
-
wild type enzyme
1150
isopropyl-thio-beta-D-galactopyranoside
-
hexahistidine-tagged Y83V mutant
2440
isopropyl-thio-beta-D-galactopyranoside
-
hexahistidine-tagged Y83L mutant
2720
isopropyl-thio-beta-D-galactopyranoside
-
hexahistidine-tagged Y83F mutant
12
p-nitrophenyl-beta-D-galactopyranoside
-
H155A variant
24.1
p-nitrophenyl-beta-D-galactopyranoside
-
hexahistidine-tagged Y83L mutant
25.2
p-nitrophenyl-beta-D-galactopyranoside
-
acetyl donor: butyrylCoA
27.2
p-nitrophenyl-beta-D-galactopyranoside
-
hexahistidine-tagged wild type enzyme
29.5
p-nitrophenyl-beta-D-galactopyranoside
-
hexahistidine-tagged Y83F mutant
40.2
p-nitrophenyl-beta-D-galactopyranoside
-
acetyl donor: acetyl-CoA
45.6
p-nitrophenyl-beta-D-galactopyranoside
-
acetyl donor: propionyl-CoA
60
p-nitrophenyl-beta-D-galactopyranoside
-
wild type enzyme
60.2
p-nitrophenyl-beta-D-galactopyranoside
-
acetyl donor: acetyl-CoA
63.4
p-nitrophenyl-beta-D-galactopyranoside
-
acetyl donor: acetyl-CoA
97
p-nitrophenyl-beta-D-galactopyranoside
-
hexahistidine-tagged Y83V mutant
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1.42
4-nitrophenyl alpha-D-galactopyranoside
-
acetyl donor: acetyl-CoA
0.006 - 11.1
4-nitrophenyl-beta-D-galactopyranoside
0.175 - 168
isopropyl-thio-beta-D-galactopyranoside
8.9
o-nitrophenyl-beta-D-galactopyranoside
-
acetyl donor: acetyl-CoA
0.006 - 14.7
p-nitrophenyl-beta-D-galactopyranoside
1.84
p-nitrophenyl-beta-D-glucopyranoside
-
acetyl donor: acetyl-CoA
1.84
p-nitrophenyl-beta-D-lactopyranoside
-
acetyl donor: acetyl-CoA
13
phenyl-beta-D-galactopyranoside
-
acetyl donor: acetyl-CoA
0.006
4-nitrophenyl-beta-D-galactopyranoside
-
25°C, mutant Y83L
0.022
4-nitrophenyl-beta-D-galactopyranoside
-
25°C, mutant Y83V
4.9
4-nitrophenyl-beta-D-galactopyranoside
-
25°C, mutant Y83F
11.1
4-nitrophenyl-beta-D-galactopyranoside
-
25°C, wild-type enzyme
0.175
isopropyl-thio-beta-D-galactopyranoside
-
hexahistidine-tagged Y83V mutant
0.31
isopropyl-thio-beta-D-galactopyranoside
-
hexahistidine-tagged Y83L mutant
122
isopropyl-thio-beta-D-galactopyranoside
-
hexahistidine-tagged wild type enzyme
133
isopropyl-thio-beta-D-galactopyranoside
-
wild type enzyme
168
isopropyl-thio-beta-D-galactopyranoside
-
hexahistidine-tagged Y83F mutant
0.006
p-nitrophenyl-beta-D-galactopyranoside
-
hexahistidine-tagged Y83L mutant
0.0082
p-nitrophenyl-beta-D-galactopyranoside
-
H155A variant
0.022
p-nitrophenyl-beta-D-galactopyranoside
-
hexahistidine-tagged Y83V mutant
0.054
p-nitrophenyl-beta-D-galactopyranoside
-
acetyl donor: butyryl-CoA
4.9
p-nitrophenyl-beta-D-galactopyranoside
-
hexahistidine-tagged Y83F mutant
5.8
p-nitrophenyl-beta-D-galactopyranoside
-
acetyl donor: propionyl-CoA
11.1
p-nitrophenyl-beta-D-galactopyranoside
-
hexahistidine-tagged wild type enzyme
14.7
p-nitrophenyl-beta-D-galactopyranoside
-
wild type enzyme
14.7
p-nitrophenyl-beta-D-galactopyranoside
-
acetyl donor: acetyl-CoA
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Zabin, I.
Crystalline thiogalactoside transacetylase
J. Biol. Chem.
238
3300-3306
1963
Escherichia coli, Escherichia coli ML308
brenda
Zabin, I.; Kepes, A.; Monod, J.
Thiogalactoside transacetylase
J. Biol. Chem.
237
253-257
1962
Escherichia coli, Escherichia coli ML308
brenda
Musso, R.E.; Zabin, I.
Substrate specificity and kinetic studies on thiogalactoside transacetylase
Biochemistry
12
553-557
1973
Escherichia coli, Escherichia coli A324-5
brenda
Zabin, I.; Fowler, A.V.
Purification of thiogalactoside transacetylase by affinity chromatography
Anal. Biochem.
136
493-496
1984
Escherichia coli
brenda
Fried, V.A.
lac Thiogalactoside transacetylase of Escherichia coli K-12 and ML
J. Bacteriol.
143
506-509
1980
Escherichia coli, Escherichia coli ML308
brenda
Fowler, A.V.; Hediger, M.A.; Musso, R.E.; Zabin, I.
The amino acid sequence of thiogalactoside transacetylase of Escherichia coli
Biochimie
67
101-108
1985
Escherichia coli
brenda
Lewendon, A.; Ellis, J.; Shaw, W.V.
Structural and mechanistic studies of galactoside acetyltransferase, the Escherichia coli LacA gene product
J. Biol. Chem.
270
26326-26331
1995
Escherichia coli
brenda
Wang, X.G.; Roderick, S.L.
Expression, purification, crystallization and preliminary x-ray data of Escherichia coli galactoside acetyltransferase
Acta Crystallogr. Sect. D
55
1955-1957
1999
Escherichia coli
brenda
Roderick, S.L.
The lac operon galactoside acetyltransferase
C. R. Biol.
328
568-575
2005
Escherichia coli
brenda
Radeghieri, A.; Bonoli, M.; Parmeggiani, F.; Hochkoeppler, A.
Tyrosine 83 is essential for the activity of E. coli galactoside transacetylase
Biochim. Biophys. Acta
1774
243-248
2007
Escherichia coli
brenda
Ballano, G.; Zanuy, D.; Jimenez, A.I.; Cativiela, C.; Nussinov, R.; Aleman, C.
Structural analysis of a beta-helical protein motif stabilized by targeted replacements with conformationally constrained amino acids
J. Phys. Chem. B
112
13101-13115
2008
Escherichia coli (P07464), Escherichia coli
brenda
Luo, H.B.; Knapik, A.A.; Petkowski, J.J.; Demas, M.; Shumilin, I.A.; Zheng, H.; Chruszcz, M.; Minor, W.
Biophysical analysis of the putative acetyltransferase SACOL2570 from methicillin-resistant Staphylococcus aureus
J. Struct. Funct. Genomics
14
97-108
2013
Staphylococcus aureus (Q5HCZ5), Staphylococcus aureus
brenda