Information on EC 2.3.1.178 - diaminobutyrate acetyltransferase

Word Map on EC 2.3.1.178
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.178
-
RECOMMENDED NAME
GeneOntology No.
diaminobutyrate acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + L-2,4-diaminobutanoate = CoA + N4-acetyl-L-2,4-diaminobutanoate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ectoine biosynthesis
-
-
Glycine, serine and threonine metabolism
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:L-2,4-diaminobutanoate N4-acetyltransferase
Requires Na+ or K+ for maximal activity [3]. Ornithine, lysine, aspartate, and alpha-, beta- and gamma-aminobutanoate cannot act as substrates [3]. However, acetyl-CoA can be replaced by propanoyl-CoA, although the reaction proceeds more slowly [3]. Forms part of the ectoine-biosynthesis pathway, the other enzymes involved being EC 2.6.1.76, diaminobutyrate---2-oxoglutarate transaminase and EC 4.2.1.108, ectoine synthase.
CAS REGISTRY NUMBER
COMMENTARY hide
130456-92-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene ectA encoded in the ectABC operon
-
-
Manually annotated by BRENDA team
gene ectA encoded in the ectABC operon
-
-
Manually annotated by BRENDA team
gene ectA encoded in the ectABC operon
-
-
Manually annotated by BRENDA team
OUT30018
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
show the reaction diagram
propionyl-CoA + L-2,4-diaminobutanoate
CoA + N4-propionyl-L-2,4-diaminobutanoate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + L-2,4-diaminobutanoate
CoA + N4-acetyl-L-2,4-diaminobutanoate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
-
10 mM, 26% inhibition, 1 mM, 18% inhibition
carbonates
-
-
-
additional information
-
no inhibition by 1 mM Cu2+
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
carbonates
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.37 - 0.375
2,4-diaminobutyrate
0.03 - 0.076
acetyl-CoA
0.365 - 0.465
L-2,4-diaminobutanoate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 10.5
-
pH 8: about 50% of maximal activity, pH 10.5: about 70% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
30 - 35
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 25
-
15°C: about 35% of maximal activity, 25°C: about 65% of maximal activity
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19700
calculated molecular mass
45000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
residual activity after a 3 h incubation at 30°C is 62% in presence of 2 M NaCl
37
-
residual activity after a 3 h incubation at 37°C is 35% in presence of 2 M NaCl
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme is stabilized in presence of NaCl. Residual activity after a 3 h incubation at 0°C is 100% in presence of 2 M NaCl
-
low stability of the purified enzyme
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4-70°C, purified recombinant His-tagged enzyme, in 50 mM Tris-HCl, pH 8.5, stable at least for 1 month at protein concentration 0.5 mg/ml
4°C or -70°C, protein concentration 0.5 mg/ml, stable for at least 1 month in 50 mM Tris-HCl, pH 8.5, containing 0.2 M KCl
-
4°C, 50 mM Tris-HCl, pH 8.5, 1 month
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
about 400fold
-
recombinant His-tagged enzyme
-
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
-
using a Ni2+-nitrilotriacetic acid-agarose and a Blue Sepharose 6FF column
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
expression in Escherichia coli with an additional six His residues at the C-terminus
-
expression in Escherichia coli, also osmoregulated in Escherichia coli
-
expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3)
-
gene ectA, ectABC operon, in Marinococcus halophilus, the transcription of ectABC genes is initiated from three individual sigma70/sigmaA-promoters locate upstream of each gene
-
gene ectA, encoded in ectABC, transcription of the ectABC-ask operon is initiated from two sigma70-dependent promoters ectAp1 and ectAp2. Gene ectR1 encodes the MarR-like transcriptional regulator EctR that is involved in the regulation of ectABC, overview
-
into the pET22b+ vector for expression in Escherichia coli BL21DE3 cells
our putative promoters (?ectA1-4) are located upstream of the ectA gene and one internal promoter PectB is upstream of the ectB gene
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
intracellular levels of ectoine increase in response to high growth temperature
-
osmotic up-shock of Halobacillus halophilus cells leads to the enhanced levels of mRNAs of ectABC
-
Show AA Sequence (235 entries)
Please use the Sequence Search for a specific query.