Information on EC 2.3.1.123 - dolichol O-acyltransferase

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The expected taxonomic range for this enzyme is: Rattus norvegicus

EC NUMBER
COMMENTARY hide
2.3.1.123
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RECOMMENDED NAME
GeneOntology No.
dolichol O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
palmitoyl-CoA + dolichol = CoA + dolichyl palmitate
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
palmitoyl-CoA:dolichol O-palmitoyltransferase
Other acyl-CoAs can also act, but more slowly. alpha-Saturated dolichols are acylated more rapidly than the alpha-unsaturated analogues.
CAS REGISTRY NUMBER
COMMENTARY hide
111839-04-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
male
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + dolichol
CoA + dolichyl acetate
show the reaction diagram
arachidonoyl-CoA + dolichol
CoA + dolichyl arachidonate
show the reaction diagram
myristoyl-CoA + dolichol
CoA + dolichyl myristate
show the reaction diagram
oleoyl-CoA + dolichol
CoA + dolichyl oleate
show the reaction diagram
palmitoyl-CoA + dolichol
CoA + dolichyl palmitate
show the reaction diagram
-
-
-
?
stearoyl-CoA + dolichol
CoA + dolichyl stearate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + dolichol
CoA + dolichyl acetate
show the reaction diagram
-
-
-
-
?
arachidonoyl-CoA + dolichol
CoA + dolichyl arachidonate
show the reaction diagram
-
-
-
-
?
myristoyl-CoA + dolichol
CoA + dolichyl myristate
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + dolichol
CoA + dolichyl oleate
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + dolichol
CoA + dolichyl palmitate
show the reaction diagram
-
-
-
?
stearoyl-CoA + dolichol
CoA + dolichyl stearate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
esterfication may play a role in targeting the lipid from the endoplasmic reticulum to lysosomes
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-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
KCl and MgCl2 have no influence
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-octylglycopyranoside
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strong, almost complete inactivation at 0.04%
Ca2+
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20 mM, 50-60% inhibition
deoxycholate
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strong, almost complete inactivation at 0.04%
Mn2+
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20 mM, 50-60% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
albumin
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activates
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Triton X-100
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activates at low concentrations by 50%
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 7
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20% of maximal activity at pH 5.0, 40% of maximal activity at pH 7.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
slight activity
Manually annotated by BRENDA team
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slight activity
Manually annotated by BRENDA team
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slight activity
Manually annotated by BRENDA team
-
slight activity
Manually annotated by BRENDA team
-
slight activity
Manually annotated by BRENDA team
-
slight activity
Manually annotated by BRENDA team
-
slight activity
Manually annotated by BRENDA team
-
slight activity
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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liver, esterification takes place on cytoplasmic side of membranes of the endoplasmic reticulum and is subsequently distributed to different organelles
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Manually annotated by BRENDA team
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Triton X-100 stabilizes
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, microsomes are stable for several months
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4°C, microsomes in 0.25 M sucrose, after 5 days 50% remaining activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE