Information on EC 2.3.1.114 - 3,4-dichloroaniline N-malonyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.114
-
RECOMMENDED NAME
GeneOntology No.
3,4-dichloroaniline N-malonyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
malonyl-CoA + 3,4-dichloroaniline = CoA + N-(3,4-dichlorophenyl)-malonamate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
-
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SYSTEMATIC NAME
IUBMB Comments
malonyl-CoA:3,4-dichloroaniline N-malonyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
94489-99-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Arachis hypogaea ssp. fastigiata
peanut
-
-
Manually annotated by BRENDA team
no activity in Triticum aestivum
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
malonyl-CoA + 2,3,4,5-tetrachloroaniline
CoA + N-(2,3,4,5-tetrachlorophenyl)-malonamate
show the reaction diagram
-
29% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 2,3,4-trichloroaniline
CoA + N-(2,3,4-trichlorophenyl)-malonamate
show the reaction diagram
-
50% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 2,4,5-trichloroaniline
CoA + N-(2,4,5-trichlorophenyl)-malonamate
show the reaction diagram
-
22% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 2,4-dichloroaniline
CoA + N-(2,4-dichlorophenyl)-malonamate
show the reaction diagram
-
53% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 2,5-dichloroaniline
CoA + N-(2,5-dichlorophenyl)-malonamate
show the reaction diagram
-
53% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 2-chloroaniline
CoA + N-(2-chlorophenyl)-malonamate
show the reaction diagram
-
61% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 3,4,5-trichloroaniline
CoA + N-(3,4,5-trichloro-phenyl)-malonamate
show the reaction diagram
-
30% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 3,4,5-trichloroaniline
CoA + N-(3,4,5-trichlorophenyl)-malonamate
show the reaction diagram
-
72% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 3,4-dichloroaniline
CoA + N-(3,4-dichlorophenyl)-malonamate
show the reaction diagram
malonyl-CoA + 3,5-dichloroaniline
CoA + N-(3,5-dichlorophenyl)-malonamate
show the reaction diagram
-
48% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 3-chloroaniline
CoA + N-(3-chlorophenyl)-malonamate
show the reaction diagram
-
67% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 4-aminophenol
CoA + N-(phenyl)-malonamate
show the reaction diagram
-
20% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 4-bromoaniline
CoA + N-(4-bromophenyl)-malonamate
show the reaction diagram
-
75% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 4-chloroaniline
CoA + N-(4-chlorophenyl)-malonamate
show the reaction diagram
-
87% of activity with 3,4-dichloroaniline
-
?
malonyl-CoA + 4-nitroaniline
CoA + N-(4-nitrophenyl)-malonamate
show the reaction diagram
-
43% of activity with 3,4-dichloroaniline
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?
malonyl-CoA + 7-amino-4-methylcoumarin
N-(4-methylcoumaryl)-7-malonamate
show the reaction diagram
-
70% of activity with 3,4-dichloroaniline
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
malonyl-CoA + 3,4-dichloroaniline
CoA + N-(3,4-dichlorophenyl)-malonamate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
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0.1 mM, complete inhibition, activity can be restored by incubation with 2 mM dithiothreitol for 30 min at 0°C
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023 - 0.1
3,4-dichloroaniline
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.143
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 7.2
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Arachis hypogaea ssp. fastigiata
-
of young seedlings
Manually annotated by BRENDA team
Arachis hypogaea ssp. fastigiata
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-
Manually annotated by BRENDA team
Arachis hypogaea ssp. fastigiata
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45000
Arachis hypogaea ssp. fastigiata
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gel filtration
47000
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1 * 47000, SDS-PAGE
48000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 47000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-cellulose, Blue Sepharose, Acrylex P-100, Matrix gel green
Arachis hypogaea ssp. fastigiata
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ammonium sulfate, ion-exchange, Sephadex G-100, hydrophobic interaction, gel filtration
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