Information on EC 2.2.1.3 - formaldehyde transketolase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.2.1.3
-
RECOMMENDED NAME
GeneOntology No.
formaldehyde transketolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-xylulose 5-phosphate + formaldehyde = D-glyceraldehyde 3-phosphate + glycerone
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
keto group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
formaldehyde assimilation III (dihydroxyacetone cycle)
-
-
Methane metabolism
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
D-xylulose-5-phosphate:formaldehyde glycolaldehydetransferase
A thiamine-diphosphate protein. Not identical with EC 2.2.1.1 transketolase. Also converts hydroxypyruvate and formaldehyde into glycerone and CO2.
CAS REGISTRY NUMBER
COMMENTARY hide
124566-23-0
-
76774-46-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
JC1 DSM 3803
-
-
Manually annotated by BRENDA team
strain KD1
-
-
Manually annotated by BRENDA team
strain JC1 DSM 3803
UniProt
Manually annotated by BRENDA team
strain JC1 DSM 3803
UniProt
Manually annotated by BRENDA team
strain DL1, methanol-grown
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetaldehyde + D-xylulose 5-phosphate
glyceraldehyde 3-phosphate + 1,3-dihydroxybutane-2-one
show the reaction diagram
-
-
-
-
?
D-erythrose 4-phosphate + D-xylulose 5-phosphate
D-fructose 6-phosphate + glyceraldehyde 3-phosphate
show the reaction diagram
-
-
-
-
?
D-ribose 5-phosphate + formaldehyde
?
show the reaction diagram
-
-
-
-
?
D-xylulose 5-phosphate + butyraldehyde
glyceraldehyde 3-phosphate + 1,3-dihydroxyhexane-2-one
show the reaction diagram
-
-
-
-
?
D-xylulose 5-phosphate + D-erythrose
glyceraldehyde 3-phosphate + D-fructose
show the reaction diagram
-
-
-
-
?
D-xylulose 5-phosphate + D-glucose
glyceraldehyde 3-phosphate + D-glycero-D-ido-oct-2-ulose
show the reaction diagram
-
-
-
-
?
D-xylulose 5-phosphate + D-glyceraldehyde
glyceraldehyde 3-phosphate + D-xylulose
show the reaction diagram
D-xylulose 5-phosphate + D-ribose
glyceraldehyde 3-phosphate + sedoheptulose
show the reaction diagram
-
-
-
-
?
D-xylulose 5-phosphate + formaldehyde
glyceraldehyde 3-phosphate + dihydroxyacetone
show the reaction diagram
D-xylulose 5-phosphate + glycolaldehyde
glyceraldehyde 3-phosphate + 1,3,5-trihydroxypentane-2-one
show the reaction diagram
D-xylulose 5-phosphate + heptaldehyde
glyceraldehyde 3-phosphate + 1,3-dihydroxynonane-2-one
show the reaction diagram
-
-
-
-
?
D-xylulose 5-phosphate + propionaldehyde
glyceraldehyde 3-phosphate + 1,3-dihydroxypentane-2-one
show the reaction diagram
-
-
-
-
?
D-xylulose 5-phosphate + ribose 5-phosphate
glyceraldehyde 3-phosphate + sedoheptulose 7-phosphate
show the reaction diagram
fructose 6-phosphate + formaldehyde
dihydroxyacetone + erythrose 4-phosphate
show the reaction diagram
glucose 6-phosphate + formaldehyde
?
show the reaction diagram
-
-
-
-
?
glycolaldehyde + D-xylulose 5-phosphate
glyceraldehyde 3-phosphate + 1,3-dihydroxypentane-2-one
show the reaction diagram
-
-
-
-
?
hydroxypyruvate + formaldehyde
CO2 + dihydroxyacetone
show the reaction diagram
ribulose 5-phosphate + formaldehyde
glyceraldehyde 3-phosphate + 1,3-dihydroxyacetone
show the reaction diagram
-
-
-
-
?
sedoheptulose 7-phosphate + formaldehyde
dihydroxyacetone + ribose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-xylulose 5-phosphate + formaldehyde
glyceraldehyde 3-phosphate + dihydroxyacetone
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thiamine diphosphate
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
activation, replaces Mg2+ to some extent
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
formaldehyde
iodoacetate
-
-
sulfate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0333 - 1
D-xylulose 5-phosphate
0.52
dihydroxyacetone
-
-
0.43 - 1.86
formaldehyde
0.42
glyceraldehyde 3-phosphate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 7.6
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
about 80% of maximal activity at pH 6.5 and 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 45
-
maximal activity at 35°C and about 80% of maximal activity at 45°C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
-
alpha4, 4 * 55000, SDS-PAGE
62500
-
alpha2, 2 * 62500, non-equilibrium pH-gradient electrophoresis followed by SDS-PAGE
65500
-
alpha2, 2 * 65500, SDS-PAGE
73000
-
alpha2, 2 * 73000, SDS-PAGE
76000
-
alpha2, 2 * 76000, SDS-PAGE
78197
x * 78197, calculated from sequence
79000
-
alpha2, 2 * 79000, SDS-PAGE
105000 - 110000
-
gel filtration
135000
-
gel filtration
140000
-
gel filtration
145000
190000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
alpha4, 4 * 55000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no evidence for glycosylation
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
t1/2: about 100 min
30
-
t1/2: about 40 min
50
-
inactivation after 10 min
70
-
rapidly inactivated
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Ca2+ does not stabilize
-
dodecanethiol stabilizes most effectively
glycerol, 20% w/v, stabilizes during purification
Mg2+ or EDTA stablizes
Mg2+ or NH4+ stabilizes for at least 80 h
-
Mg2+, 5 mM, thiamine diphosphate 0.5 mM, DTT, 1 mM, EDTA 1 mM stabilize
-
PMSF, 0.024% w/v, stabilizes
-
thiamine diphosphate, 0.005% w/v, stabilizes slightly
-
unstable, formaldehyde, EDTA, ammonium sulfate, methanol, ethanol, DTT, xylulose 5-phosphate and PMSF do not stabilize
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, t1/2: 45 h
-
4°C, at least 2-3 weeks under 1 ml dodecanethiol
4°C, inactivation within a few h in the absence of dodecanethiol
4°C, partially purified, inactivation within 12 h
-
5°C, at least 1 month in buffer with stabilizing agents
-
5°C, inactivation within 10 days with one of the stabilizing agents of the buffer omitted
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
spheroblast and peroxisome lysis
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli JM109
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
overexpression of dihydroxyacetone synthase and dihydroxyacetone kinase in Nicotiana tabacum achieves high expression in leaf. Targeting to chloroplasts leads to creation of a photosynthetic formaldehyde-assimilation pathway in transgenic tobacco