Information on EC 2.1.3.7 - 3-hydroxymethylcephem carbamoyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.3.7
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RECOMMENDED NAME
GeneOntology No.
3-hydroxymethylcephem carbamoyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
carbamoyl phosphate + a 3-hydroxymethylceph-3-em-4-carboxylate = phosphate + a 3-carbamoyloxymethylcephem
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carbamoyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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cephamycin C biosynthesis
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Penicillin and cephalosporin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
carbamoyl-phosphate:3-hydroxymethylceph-3-em-4-carboxylate carbamoyltransferase
Acts on a wide range of 3-hydroxymethylcephems (a subclass of the cephalosporin antibiotics). Activated by ATP.
CAS REGISTRY NUMBER
COMMENTARY hide
74315-96-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Streptomyces griseus
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Manually annotated by BRENDA team
no activity in Streptomyces lipmanii
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
carbamoyl phosphate + 7-aminodeacetylcephalosporanic acid
7-beta-acylamino-3-carbamoyloxymethylcephem + phosphate
show the reaction diagram
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?
carbamoyl phosphate + 7beta-acylamino-3-hydroxymethylcephem
7beta-acylamino-3-carbamoyloxymethylcephem + phosphate
show the reaction diagram
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?
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate
phosphate + a 3-carbamoyloxymethylcephem
show the reaction diagram
carbamoyl phosphate + decarbamoylcefuroxime
carbamoylcefuroxime + phosphate
show the reaction diagram
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-
?
carbamoyl phosphate + [3-hydroxymethyl]deacetylcephalosporin C
7beta-(5-D-aminoadipamido)-3-carbamoyloxymethylceph-3-em-4-carboxylic acid + phosphate
show the reaction diagram
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ATP may be a substrate or an effector
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
carbamoyl phosphate + a 3-hydroxymethyl ceph-3-em-4-carboxylate
phosphate + a 3-carbamoyloxymethylcephem
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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activation, up to 1 mM, may be substrate or effector, maximal activity in the presence of Mg2+ and Mn2+
dATP
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activation, can replace ATP to some extent
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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activation, maximal activity in the presence of Mn2+ and ATP
Mn2+
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activation, maximal activity in the presence of Mg2+ and ATP
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine-alpha,beta-methylene triphosphate
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adenosine-beta,gamma-methylene triphosphate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9 - 7.4
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about 75% of maximal activity at pH 5.9 and 7.4
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57149
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x * 57149, calculation from nucleotide sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 57149, calculation from nucleotide sequence
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
phosphate anions stabilize during purification
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 50 mM phosphate buffer, pH 7.0, stable for several weeks
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4°C, in the presence of ATP and carbamoyl phosphate, stable for at least 3 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cmcH subcloned in pIJ702 and expressed in Streptomyces lividans
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