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Information on EC 2.1.3.10 - malonyl-S-ACP:biotin-protein carboxyltransferase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Malonomonas rubra
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malonyl-S-ACP:biotin-protein carboxyltransferase
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a malonyl-[acyl-carrier protein] + a biotinyl-[protein] = an acetyl-[acyl-carrier protein] + a carboxybiotinyl-[protein]
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malonate degradation II (biotin-dependent)
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malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxytransferase
Derived from the components MadC and MadD of the anaerobic bacterium Malonomonas rubra, this enzyme is a component of EC 4.1.1.89, biotin-dependent malonate decarboxylase. The carboxy group is transferred from malonate to the prosthetic group of the biotin protein (MadF) with retention of configuration [2]. Similar to EC 4.1.1.87, malonyl-S-ACP decarboxylase, which forms part of the biotin-independent malonate decarboxylase (EC 4.1.1.88), this enzyme also follows on from EC 2.3.1.187, acetyl-S-ACP:malonate ACP transferase, and results in the regeneration of the acetyl-[acyl-carrier protein] [3].
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acetyl-S-acyl carrier protein:malonate acyl carrier protein-SH transferase
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malonyl-S-acyl-carrier protein:biotin-protein carboxyltransferase
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malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxyltransferase
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80700-20-5
multienzyme complex malonate decarboxylase
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enzyme is part of the Na+-activied malonate decarboxylase system
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brenda
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acetyl-CoA + dephospho-CoA
CoA + acetyl-dephospho-CoA
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r
malonyl-CoA + dephospho-CoA
CoA + malonyl-dephospho-CoA
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r
additional information
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enzyme catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
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acetyl-CoA + dephospho-CoA
CoA + acetyl-dephospho-CoA
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r
malonyl-CoA + dephospho-CoA
CoA + malonyl-dephospho-CoA
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r
additional information
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enzyme catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
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4.5
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sharp decrease in activity from pH 5.5 to pH 4.5
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Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
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67000
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1 * 67000, SDS-PAGE
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monomer
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1 * 67000, SDS-PAGE
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purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
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MADC_MALRU
318
34900
Swiss-Prot
MADD_MALRU
292
31829
Swiss-Prot
A0A0D8FTA5_9ACTN
562
59977
TrEMBL
A0A1J5S2L5_9ZZZZ
240
25729
TrEMBL
A0A1J5Q9U9_9ZZZZ
273
29095
TrEMBL
A0A1Q2YVW9_9PROT
304
32477
TrEMBL
A0A0K8LV57_PSESF
266
28381
TrEMBL
A0A0C6EV56_PSEAI
268
28497
TrEMBL
A0A1V5ULC2_9BACT
232
25069
TrEMBL
A0A1J5S2T0_9ZZZZ
298
31721
TrEMBL
A0A109L483_PSEFL
256
27350
TrEMBL
A0A1E7WA48_9BURK
284
29893
TrEMBL
A0A1A8TKN7_9GAMM
275
29511
TrEMBL
A0A1E7V327_9BURK
238
25687
TrEMBL
A0A1J5TEX5_9ZZZZ
298
31505
TrEMBL
A0A1W7BFF0_OXAFO
277
29682
TrEMBL
A0A0S4HW14_9PSED
283
30120
TrEMBL
A0A0D0TNA4_PSEFL
280
29820
TrEMBL
A0A0B7DFI2_PSEFL
283
30339
TrEMBL
A0A149VQ62_9PROT
244
27133
TrEMBL
A0A1N7SFI2_9BURK
294
31246
TrEMBL
A0A0R0LG07_9PROT
246
27065
TrEMBL
A0A1Y6H2Z0_9XANT
234
24598
TrEMBL
A0A1X9MCD7_9BACI
563
61331
TrEMBL
A0A0S4HW52_9PSED
267
28126
TrEMBL
A0A1R7QFX4_ACIJO
295
32006
TrEMBL
A0A1R7QFQ4_ACIJO
238
26357
TrEMBL
A0A0R0LMY0_9PROT
298
32433
TrEMBL
A0A1E7US92_9BURK
270
28577
TrEMBL
A0A1E7V970_9BURK
284
29778
TrEMBL
A0A109L484_PSEFL
280
29698
TrEMBL
A0A109KKM3_PSEFL
280
29652
TrEMBL
A0A0B7DGD0_PSEFL
261
27893
TrEMBL
A0A1Y6H3K4_9XANT
306
32239
TrEMBL
A0A1Q9R2V6_PSEPU
260
27451
TrEMBL
A0A1A8TK30_9GAMM
284
30209
TrEMBL
A0A1J5TT98_9ZZZZ
240
25530
TrEMBL
A0A1J5PZD1_9ZZZZ
279
29385
TrEMBL
A0A150HQ72_9GAMM
283
30620
TrEMBL
A0A071KS24_PSEAI
287
30343
TrEMBL
A0A0M3F9X0_ACIBA
283
30608
TrEMBL
A0A1E7V8H7_9BURK
270
28527
TrEMBL
A0A150HQE9_9GAMM
283
30650
TrEMBL
A0A150HQA8_9GAMM
275
30355
TrEMBL
A0A1E7X4Y4_9BURK
277
29207
TrEMBL
A0A1E7WQ92_9BURK
300
31628
TrEMBL
A0A0K8LTF4_PSESF
283
29934
TrEMBL
A0A1E7WA69_9BURK
270
28790
TrEMBL
A0A0D0TUQ4_PSEFL
256
27313
TrEMBL
A0A109KKM4_PSEFL
256
27276
TrEMBL
A0A1E7USB7_9BURK
284
29666
TrEMBL
A0A1V5ULB2_9BACT
295
31769
TrEMBL
A0A1J5S6R6_9ZZZZ
240
25801
TrEMBL
A0A161WF25_9CLOT
565
61279
TrEMBL
A0A125QIT5_PSEFL
256
27391
TrEMBL
A0A0M3FQN4_ACIBA
275
30046
TrEMBL
A0A149VPZ7_9PROT
298
31910
TrEMBL
A0A1J5SJE9_9ZZZZ
298
31689
TrEMBL
A0A150HPV3_9GAMM
275
30235
TrEMBL
A0A0P1D5E0_PSEAI
268
28497
TrEMBL
A0A0M2WGR6_9BURK
284
29771
TrEMBL
A0A1R4B5Q1_9VIBR
265
28600
TrEMBL
A0A120G8D1_PSEFL
121
13330
TrEMBL
A0A1Q2YVH0_9PROT
242
25145
TrEMBL
A0A1Q9R2Z8_PSEPU
284
29979
TrEMBL
A0A0M2WJR2_9BURK
270
28567
TrEMBL
A0A1E7V2W8_9BURK
300
31673
TrEMBL
A0A1E7X4W5_9BURK
281
29756
TrEMBL
A0A1E7WQH6_9BURK
238
25673
TrEMBL
A0A1W7BES6_OXAFO
273
29511
TrEMBL
A0A0P1D5N5_PSEAI
287
30382
TrEMBL
Q9I6S6_PSEAE
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
268
28497
TrEMBL
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Hilbi, H.; Dimroth, P.
Purification and characterization of a cytoplasmic enzyme component of the Na+-activated malonate decarboxylase system of Malonomonas rubra: acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Arch. Microbiol.
162
48-56
1994
Malonomonas rubra
brenda
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