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Information on EC 2.1.1.B83 - 7'-O-demethylcephaeline 6',7'-dimethyltransferase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Carapichea ipecacuanha
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2.1.1.B83
preliminary BRENDA-supplied EC number
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7'-O-demethylcephaeline 6',7'-dimethyltransferase
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2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline = 2 S-adenosyl-L-homocysteine + emetine
S-adenosyl-L-methionine + 7'-O-demethylcephaeline = S-adenosyl-L-homocysteine + cephaeline
S-adenosyl-L-methionine + cephaeline = S-adenosyl-L-homocysteine + emetine
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline = 2 S-adenosyl-L-homocysteine + emetine
overall reaction
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline = 2 S-adenosyl-L-homocysteine + emetine
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S-adenosyl-L-methionine + 7'-O-demethylcephaeline = S-adenosyl-L-homocysteine + cephaeline
(1a)
S-adenosyl-L-methionine + 7'-O-demethylcephaeline = S-adenosyl-L-homocysteine + cephaeline
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S-adenosyl-L-methionine + cephaeline = S-adenosyl-L-homocysteine + emetine
(1b)
S-adenosyl-L-methionine + cephaeline = S-adenosyl-L-homocysteine + emetine
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S-adenosyl-L-methionine:7'-O-demethylcephaeline 6',7'-O-dimethyltransferase
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7'-O-demethylcephaeline O-dimethyltransferase
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7'-O-demethylcephaeline/cephaeline 6',7'-methyltransferase
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UniProt
brenda
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2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline
2 S-adenosyl-L-homocysteine + emetine
S-adenosyl-L-methionine + 7'-O-demethylcephaeline
S-adenosyl-L-homocysteine + cephaeline
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S-adenosyl-L-methionine + cephaeline
2 S-adenosyl-L-homocysteine + emetine
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2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline
2 S-adenosyl-L-homocysteine + emetine
the enzyme is involved in biosynthesis of emetine
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2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline
2 S-adenosyl-L-homocysteine + emetine
the enzyme is involved in biosynthesis of emetine. Almost all of the 7'-O-demethylcephaeline is converted to cephaline within 2 min. Emetine the methylation product of cephaline is not observed, which indicates thatCiOMT1 does not use cephaline as a preferred substrate compared to 7'-O-demethylcephaeline. When all of the 7'-O-demethylcephaeline is converted to cephaline after 10 min, emetine is gradually produced from cephaline
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2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline
2 S-adenosyl-L-homocysteine + emetine
E7FL15
the enzyme is involved in biosynthesis of emetine
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additional information
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CiOMT does not require divalent cations for activity. Ca2+ and Mg2+ have no effect on activity
additional information
CiOMT does not require divalent cations for activity. Ca2+ and Mg2+ have no effect on activity
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Co2+
5 mM, 53% inhibition
Mn2+
5 mM, 31% inhibition
Ni2+
5 mM, 83% inhibition
Zn2+
5 mM, 90% inhibition
additional information
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EDTA has no effect on activity, negligibly inhibited by iodoacetamide
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additional information
EDTA has no effect on activity, negligibly inhibited by iodoacetamide
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0.068
cephaeline
pH 6.8, 30°C
0.097
S-adenosyl-L-methionine
pH 6.8, 30°C
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6.8
cephaline as substrate
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brenda
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40000
x * 40000, SDS-PAGE
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expression in Escherichia coli
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E7FL15_9GENT
350
39081
TrEMBL
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Cheong, B.E.; Takemura, T.; Yoshimatsu, K.; Sato, F.
Molecular cloning of an O-methyltransferase from adventitious roots of Carapichea ipecacuanha
Biosci. Biotechnol. Biochem.
75
107-113
2011
Carapichea ipecacuanha, Carapichea ipecacuanha (E7FL15)
brenda
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