Information on EC 2.1.1.B83 - 7'-O-demethylcephaeline 6',7'-dimethyltransferase

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The expected taxonomic range for this enzyme is: Carapichea ipecacuanha

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EC NUMBER
COMMENTARY hide
2.1.1.B83
preliminary BRENDA-supplied EC number
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RECOMMENDED NAME
GeneOntology No.
7'-O-demethylcephaeline 6',7'-dimethyltransferase
-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline = 2 S-adenosyl-L-homocysteine + emetine
show the reaction diagram
S-adenosyl-L-methionine + 7'-O-demethylcephaeline = S-adenosyl-L-homocysteine + cephaeline
show the reaction diagram
S-adenosyl-L-methionine + cephaeline = S-adenosyl-L-homocysteine + emetine
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:7'-O-demethylcephaeline 6',7'-O-dimethyltransferase
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline
2 S-adenosyl-L-homocysteine + emetine
show the reaction diagram
S-adenosyl-L-methionine + 7'-O-demethylcephaeline
S-adenosyl-L-homocysteine + cephaeline
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + cephaeline
2 S-adenosyl-L-homocysteine + emetine
show the reaction diagram
-
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl-L-methionine + 7'-O-demethylcephaeline
2 S-adenosyl-L-homocysteine + emetine
show the reaction diagram
E7FL15
the enzyme is involved in biosynthesis of emetine
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
5 mM, 53% inhibition
Mn2+
5 mM, 31% inhibition
Ni2+
5 mM, 83% inhibition
Zn2+
5 mM, 90% inhibition
additional information
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.068
cephaeline
pH 6.8, 30°C
0.097
S-adenosyl-L-methionine
pH 6.8, 30°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8
cephaline as substrate
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
x * 40000, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 40000, SDS-PAGE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.B83)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)