Information on EC 2.1.1.B74 - apigenin 7-O-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.B74
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
apigenin 7-O-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone = S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:5,7,4'-trihydroxyflavone 7-O-methyltransferase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
inoculated with the pathogenic fungus Blumeria graminis
UniProt
Manually annotated by BRENDA team
gene POMT-7
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the enzyme catalyzes the last step in genkwanin biosynthesis from tyrosine
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3',4',5,7-tetrahydroxyflavanone
S-adenosyl-L-homocysteine + 3',4',5-trihydroxy-7-methoxyflavanone
show the reaction diagram
i.e. eriodictyol, about 10% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin)
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-
?
S-adenosyl-L-methionine + 3,4',5,7-tetrahydroxyflavanone
S-adenosyl-L-homocysteine + 3,4',5-trihydroxy-7-methoxyflavanone
show the reaction diagram
i.e. kaempferol, about 15% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin)
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S-adenosyl-L-methionine + 5,7,3',4'-tetrahydroxyflavone
S-adenosyl-L-homocysteine + 7-methoxy-5,3',4'-tetrahydroxyflavone
show the reaction diagram
i.e. luteolin, about 15% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin)
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-
?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavanone
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavanone
show the reaction diagram
i.e. naringenin, about 30% of the activity with 5,7,4'-trihydroxyflavone (i.e. apigenin)
i.e. sakuranetin
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?
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone
show the reaction diagram
S-adenosyl-L-methionine + apigenin
S-adenosyl-L-homocysteine + 7-O-methylapigenin
show the reaction diagram
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i.e. genkwanin
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 5,7,4'-trihydroxyflavone
S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyflavone
show the reaction diagram
Q43771
i.e. apigenin. The product genkwanin exhibits a stronger inhibitory effect on the germination of spores from pathogenic fungi as compared to the effect of apigenin
i.e. genkwanin
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?
S-adenosyl-L-methionine + apigenin
S-adenosyl-L-homocysteine + 7-O-methylapigenin
show the reaction diagram
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i.e. genkwanin
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0046
5,7,4'-trihydroxyflavone
pH 7.8, 20°C
0.0109
S-adenosyl-L-methionine
pH 7.8, 20°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
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recombinant enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
expressed in barley leaves in response to attack by the pathogenic fungus Blumeria graminis. NOt detected in healthy plants
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42300
calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 42300, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene POMT-7, recombinant expression of His-tagged enzyme in Escherichia coli
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recombinant expression of tagged enzyme in Escherichia coli, coexpression with tyrosine ammonia lyase, 4-coumaroyl coenzyme A ligase, chalcone synthase, chalcone isomerase, flavone synthase, and apigenin 7-O-methyltransferase to resonstitute genkwanin production from tyrosine in transformed Escherichia coli, method, overview
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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coexpression of the apigenin 7-O-methyltransferase with tyrosine ammonia lyase, 4-coumaroyl coenzyme A ligase, chalcone synthase, chalcone isomerase,and flavone synthase to resonstitute genkwanin production from tyrosine in transformed Escherichia coli with modulated tyrosine content, method, overview