Information on EC 2.1.1.91 - isobutyraldoxime O-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.91
-
RECOMMENDED NAME
GeneOntology No.
isobutyraldoxime O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 2-methylpropanal oxime = S-adenosyl-L-homocysteine + 2-methylpropanal O-methyloxime
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:2-methylpropanal-oxime O-methyltransferase
Oximes of C4 to C6 aldehydes can act as acceptors; the most active substrate is 2-methylbutyroaldoxime.
CAS REGISTRY NUMBER
COMMENTARY hide
95471-32-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
N.C.I.B. 11652
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-
Manually annotated by BRENDA team
N.C.I.B. 11652
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-methylbutyraldoxime + S-adenosyl-L-methionine
2-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine
show the reaction diagram
3-methylbutyraldoxime + S-adenosyl-L-methionine
3-methylbutyraldoxime methyl ether + S-adenosyl-L-homocysteine
show the reaction diagram
isobutyraldoxime + S-adenosyl-L-methionine
isobutyraldoxime methyl ether + S-adenosyl-L-homocysteine
show the reaction diagram
methacrylaldoxime + S-adenosyl-L-methionine
methacrylaldoxime methyl ether + S-adenosyl-L-homocysteine
show the reaction diagram
-
-
-
?
n-butyraldoxime + S-adenosyl-L-methionine
n-butyraldoxime methyl ether + S-adenosyl-L-homocysteine
show the reaction diagram
-
-
-
?
n-pentanaldoxime + S-adenosyl-L-methionine
n-pentanaldoxime methyl ether + S-adenosyl-L-homocysteine
show the reaction diagram
-
-
-
?
n-propionaldoxime + S-adenosyl-L-methionine
n-propionaldoxime methyl ether + S-adenosyl-L-homocysteine
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
Mg2+ has no significant effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag2+
-
100% inhibition at 1 mM
Hg2+
-
100% inhibition at 0.05 mM
iodoacetamide
-
55% inhibition at 0.5 mM
N-ethylmaleimide
-
56% inhibition at 0.05 mM
Phenylmercuriacetate
-
95% inhibition at 0.025 mM
S-adenosyl-L-homocysteine
-
competitive inhibition
additional information
-
EDTA and Mg2+ are not inhibitory
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
requires thiol-protecting agents for activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14
2-Methylbutyraldoxime
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-
0.16
3-Methylbutyraldoxime
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-
0.24 - 0.27
Isobutyraldoxime
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-
1.11
Methacrylaldoxime
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-
0.98
n-Butyraldoxime
-
-
0.67
n-Pentanaldoxime
-
-
1.54
n-Propionaldoxime
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-
0.15
S-adenosyl-L-methionine
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-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.027
S-adenosyl-L-homocysteine
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9 - 10.4
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less than 50% of maximal activity below pH 9.0, almost no activity above pH 10.4
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
84000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
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rapid inactivation above
additional information
-
temperature sensitive: 0-3°C are necessary during purification
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
labile to O2, thiol-protecting agents are necessary
-
485667
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 100 mM potassium phosphate buffer, pH 7.5, 5 mM DTT, several months
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0°C, (NH4)2SO4-paste has a half life of 10 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE