Information on EC 2.1.1.88 - 8-hydroxyquercetin 8-O-methyltransferase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.1.1.88
-
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RECOMMENDED NAME
GeneOntology No.
8-hydroxyquercetin 8-O-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 3,5,7,8,3',4'-hexahydroxyflavone = S-adenosyl-L-homocysteine + 3,5,7,3',4'-pentahydroxy-8-methoxyflavone
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
gossypetin metabolism
-
-
phenylpropanoids methylation (ice plant)
-
-
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:3,5,7,8,3',4'-hexahydroxyflavone 8-O-methyltransferase
Also acts on 8-hydroxykaempferol, but not on the glycosides of 8-hydroxyflavonols. An enzyme from the flower buds of Lotus corniculatus.
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CAS REGISTRY NUMBER
COMMENTARY hide
99775-17-4
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone
S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone
show the reaction diagram
S-adenosyl-L-methionine + 8-hydroxykaempferol
S-adenosyl-L-homocysteine + 8-methoxykaempferol
show the reaction diagram
-
-
-
?
additional information
?
-
-
not: glycosides of 8-hydroxyflavonols
-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone
S-adenosyl-L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + 8-hydroxykaempferol
S-adenosyl-L-homocysteine + 8-methoxykaempferol
show the reaction diagram
-
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-Methoxykaempferol
-
product inhibition, competitive with respect to 8-hydroxykaempferol
EDTA
-
in absence of Mg2+
S-adenosyl-L-homocysteine
-
product inhibition, non-competitive with respect to S-adenosyl-L-methionine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0013
8-Hydroxykaempferol
-
-
0.053
S-adenosyl-L-methionine
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
-
gel filtration
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.88)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)