Information on EC 2.1.1.86 - tetrahydromethanopterin S-methyltransferase

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The expected taxonomic range for this enzyme is: Euryarchaeota

EC NUMBER
COMMENTARY hide
2.1.1.86
-
RECOMMENDED NAME
GeneOntology No.
tetrahydromethanopterin S-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate = 5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Methane metabolism
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-
methanogenesis from H2 and CO2
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-
Microbial metabolism in diverse environments
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methanogenesis from CO2
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-
SYSTEMATIC NAME
IUBMB Comments
5-methyl-5,6,7,8-tetrahydromethanopterin:2-mercaptoethanesulfonate 2-methyltransferase
Involved in the formation of methane from CO in Methanobacterium thermoautotrophicum. Methanopterin is a pterin analogue. The reaction involves the export of one or two sodium ions in Archaea.
CAS REGISTRY NUMBER
COMMENTARY hide
103406-60-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
CmtA is a soluble 5-methyl-5,6,7,8-tetrahydrosarcinapterin:coenzyme M methyltransferase postulated to supplement the membrane-bound 5-methyl-5,6,7,8-tetrahydromethanopterin:coenzyme M methyltransferase during CO-dependent growth of Methanosarcina acetivorans
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + H+
show the reaction diagram
-
-
-
-
r
5,6,7,8-tetrahydromethanopterin + methylcob(III)alamin
5-methyl-5,6,7,8-tetrahydromethanopterin + cob(III)alamin
show the reaction diagram
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
show the reaction diagram
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M
5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
show the reaction diagram
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + H+
5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
show the reaction diagram
-
-
-
-
r
5-methyl-5,6,7,8-tetrahydrosarcinapterin + coenzyme M
5,6,7,8-tetrahydrosarcinapterin + methyl-coenzyme M
show the reaction diagram
-
-
-
?
methylcobalamin + 2-mercaptoethanesulfonate
?
show the reaction diagram
N5-methyltetrahydrofolate + 2-mercaptoethanesulfonate
?
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + H+
show the reaction diagram
-
-
-
-
r
5-methyl-5,6,7,8-tetrahydromethanopterin + 2-mercaptoethanesulfonate
5,6,7,8-tetrahydromethanopterin + 2-(methylthio)ethanesulfonate
show the reaction diagram
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M
5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
show the reaction diagram
Q8THX4
-
-
-
?
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + H+
5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M
show the reaction diagram
-
-
-
-
r
5-methyl-5,6,7,8-tetrahydrosarcinapterin + coenzyme M
5,6,7,8-tetrahydrosarcinapterin + methyl-coenzyme M
show the reaction diagram
Q8THX4
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
corrinoid
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Na+
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
maximum activity at 1 mM Na+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.7
(2-methylthio)ethanesulfonate
-
-
0.88
5,6,7,8-tetrahydromethanopterin
-
-
0.135
5-Methyl-5,6,7,8-tetrahydromethanopterin
at 37°C in 50 mM MOPS-KOH (pH 7.0)
0.277
coenzyme M
at 37°C in 50 mM MOPS-KOH (pH 7.0)
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.4
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
37°C, pH 6.9
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
-
sharp decrease in activity below pH 6.0 and above pH 7.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9000
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
12000
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
13000
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
20000
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
28000
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
34000
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
x * 34000 + x * 28000 + x * 20000 + x * 13000 + x * 12000 + x * 9000, SDS-PAGE
69000
x * 69000, SDS-PAGE
380000
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
non-denaturing gradient PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
during purification, the enzyme is stabilized with 0.01 mM hydroxocobalamin
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
oxygen-sensitive
-
639489
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
P80655 and P80653 and P80651 and P80654 and P80656 and P80650
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
all of these subunits are shown to be heterologously expressed in minicells of the Escherichia coli mutant DK6. Sequence comparisons with the methyltransferases of thermophilic and hypothermophilic methanogenic archaea
O59640 and P80655 and O59638 and P80653 and P80651 and P80654 and P80656 and P80650
expressed in Escherichia coli Rosetta (DE3) cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTAmtr
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mutants lacking H4MPT:coenzyme M methyltransferase-encoding operon (DELTAmtr), the methylene-H4MPT reductase encoding gene (DELTAmer), the methylene-H4MPT dehydrogenase-encoding gene (DELTAmtd), and the formyl-methanofuran: H4MPT formyl-transferase-encoding gene (DELTAftr) fail to grow using either methanol or H2/CO2 as a growth substrate, indicating that there is an absolute requirement for the C1 oxidation/reduction pathway for hydrogenotrophic and methylotrophic methanogenesis. Mutants also fail to grow on acetate. DELTAmtr and DELTAmer mutants are capable of producing methane from methanol. Thus, there is an Mtr/Mer bypass pathway that allows oxidation of methanol to the level of methylene-H4MPT in Methanosarcina barkeri. Formaldehyde may be an intermediate in this bypass. DELTAmer and DELTAmtr mutants are able to grow on a combination of methanol plus acetate
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