Information on EC 2.1.1.83 - 3,7-dimethylquercetin 4'-O-methyltransferase

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The expected taxonomic range for this enzyme is: Chrysosplenium americanum

EC NUMBER
COMMENTARY hide
2.1.1.83
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RECOMMENDED NAME
GeneOntology No.
3,7-dimethylquercetin 4'-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 5,3',4'-trihydroxy-3,7-dimethoxyflavone = S-adenosyl-L-homocysteine + 5,3'-dihydroxy-3,7,4'-trimethoxyflavone
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Flavone and flavonol biosynthesis
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polymethylated quercetin glucoside biosynthesis I - quercetin series (Chrysosplenium)
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:5,3',4'-trihydroxy-3,7-dimethoxyflavone 4'-O-methyltransferase
3,7-Dimethylquercetagetin can also act as acceptor. Involved with EC 2.1.1.76 quercetin 3-O-methyltransferase and EC 2.1.1.82 3-methylquercetin 7-O-methyltransferase in the methylation of quercetin to 3,7,4'-trimethylquercetin in Chrysosplenium americanum. Does not act on flavones, dihydroflavonols, or their glucosides.
CAS REGISTRY NUMBER
COMMENTARY hide
96477-60-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3',4',5-trihydroxy-3,7-dimethoxyflavone
S-adenosyl-L-homocysteine + 3',5-dihydroxy-3,4',7-trimethoxyflavone
show the reaction diagram
S-adenosyl-L-methionine + 3,7-dimethylquercetagetin
S-adenosyl-L-homocysteine + 3,7,4'-trimethylquercetagetin + H+
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3',4',5-trihydroxy-3,7-dimethoxyflavone
S-adenosyl-L-homocysteine + 3',5-dihydroxy-3,4',7-trimethoxyflavone
show the reaction diagram
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trivial name 3,7-dimethylquercetin, enzyme does not act on flavones, dihydroflavonols or their glucosides, involved with EC 2.1.1.76 and 2.1.1.82 in the methylation of quercitin to 3,7,4'-trimethylquercitin
trivial name 3,7,4'-trimethylquercetin
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3',5-dihydroxy-3,4,7'-trimethoxyflavone
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N-ethylmaleimide
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p-chloromercuribenzoate
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S-adenosyl-L-homocysteine
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product inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.015
3,7-dimethylquercetin
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0.13
S-adenosyl-L-methionine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 9.5
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approx. 50% of maximal activity at pH 7.2 and 9.5
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57000
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gel filtration
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 200 mM potassium phosphate buffer, pH 8, 20% glycerol, 5 mM dithiothreitol, several months, no loss of activity
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4°C, 4-6 weeks, 80% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
chromatofocusing on Superose 12 and Mono P
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