Information on EC 2.1.1.78 - isoorientin 3'-O-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Silene latifolia subsp. alba

EC NUMBER
COMMENTARY hide
2.1.1.78
-
RECOMMENDED NAME
GeneOntology No.
isoorientin 3'-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + isoorientin = S-adenosyl-L-homocysteine + isoscoparin
show the reaction diagram
also acts on isoorientin 2''-O-rhamnoside. Involved in the biosynthesis of flavones
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:isoorientin 3'-O-methyltransferase
Also acts on isoorientin 2''-O-rhamnoside. Involved in the biosynthesis of flavones.
CAS REGISTRY NUMBER
COMMENTARY hide
83061-51-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + isoorientin
S-adenosyl-L-homocysteine + isoscoparin
show the reaction diagram
S-adenosyl-L-methionine + isoorientin 2''-O-rhamnoside
S-adenosyl-L-homocysteine + isoscoparin 2''-O-rhamnoside
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + isoorientin
S-adenosyl-L-homocysteine + isoscoparin
show the reaction diagram
-
involved in biosynthesis of flavones
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
slightly stimulatory
Mn2+
-
slightly stimulatory
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001
caffeic acid
-
second substrate caffeic acid
0.32
isoorientin
-
-
0.007
isoorientin 2''-O-rhamnoside
-
second substrate S-adenosyl-L-methionine
0.0012 - 0.005
S-adenosyl-L-methionine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
-
isoorientin as substrate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 55
-
35% of maximum activity at 30°C and 55°C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
after 20 min: 55% activity remains, after 40 min 20% activity remains
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE