Information on EC 2.1.1.61 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.1.1.61
-
RECOMMENDED NAME
GeneOntology No.
tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (5-methylaminomethyl-2-thio-uridylate)-methyltransferase
This enzyme is specific for the terminal methyl group of 5-methylaminomethyl-2-thiouridylate.
CAS REGISTRY NUMBER
COMMENTARY hide
39391-17-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene trmU
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 5-aminomethyl-2-thiouridine in tRNA
S-adenosyl-L-homocysteine + 5-methylaminomethyl-2-thiouridylate in tRNA
show the reaction diagram
additional information
?
-
-
the enzyme exhibits two activities: enzyme from trmC1 mutant reacts with 5-carboxymethylaminomethyl-2-thiouridine to form 5-aminomethyl-2-thiouridine without S-adenosyl-L-methionine, enzyme from trmC2 mutant methylates 5-aminomethyl-2-thiouridine in the presence of S-adenosyl-L-methionine
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 5-aminomethyl-2-thiouridine in tRNA
S-adenosyl-L-homocysteine + 5-methylaminomethyl-2-thiouridylate in tRNA
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NH4+
-
activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg2+
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strong, 5 mM
NaCl
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mutant trmC1: 100 mM, mutant trmC2: 200 mM
p-chloromercuribenzoate
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-
additional information
-
no inhibition by putrescine or spermidine
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no activation by putrescine or spermidine
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.23
5-aminomethyl-2-thiouridine
Escherichia coli
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.1686
-
mutant trmC1
0.9252
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mutant trmC2
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 8.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.7
amino acid sequence calculation
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
low level
Manually annotated by BRENDA team
abundantly expressed in tissues with high metabolic rates
Manually annotated by BRENDA team
low level
Manually annotated by BRENDA team
low level
Manually annotated by BRENDA team
moderately expressed
Manually annotated by BRENDA team
abundantly expressed in tissues with high metabolic rates
Manually annotated by BRENDA team
moderately expressed
Manually annotated by BRENDA team
moderately expressed
Manually annotated by BRENDA team
low level
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47240
x * 47240, amino acid sequence calculation
47750
sequence analysis
79000
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1 * 79000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 47240, amino acid sequence calculation
monomer
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1 * 79000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
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affinity chromatography; on Heparin-Sepharose
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning and mapping of trmC gene
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gene trmU, DNA and amino acid sequence determination and analysis, expression in NIH3T3 cells mitochondria as N-terminal GFP-fusion protein
TRMU-GFP fusion protein transfected into 143B cell line
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A10S
-
the mutation is associated with acute liver failure in infancy
G14S
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the mutation is associated with acute liver failure in infancy
G272D
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the mutation is associated with acute liver failure in infancy
L233F/A10S
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the mutation is associated with acute liver failure in infancy
M1K
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the mutation is associated with acute liver failure in infancy
V279M
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the mutation is associated with acute liver failure in infancy
Y77H
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the mutation is associated with acute liver failure in infancy
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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