Information on EC 2.1.1.54 - deoxycytidylate C-methyltransferase

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The expected taxonomic range for this enzyme is: Xanthomonas oryzae

EC NUMBER
COMMENTARY hide
2.1.1.54
-
RECOMMENDED NAME
GeneOntology No.
deoxycytidylate C-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5,10-methylenetetrahydrofolate + dCMP = dihydrofolate + deoxy-5-methylcytidylate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
5,10-methylenetetrahydrofolate:dCMP C-methyltransferase
dCMP is methylated by formaldehyde in the presence of tetrahydrofolate. CMP, dCTP and CTP can act as acceptors, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
61970-01-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
phage Xp12-infected
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,10-methylenetetrahydrofolate + CMP
dihydrofolate + 5-methylcytidylate
show the reaction diagram
-
-
-
-
?
5,10-methylenetetrahydrofolate + CTP
dihydrofolate + 5-methylcytidine 5'-triphosphate
show the reaction diagram
-
low activity
-
-
-
5,10-methylenetetrahydrofolate + dCMP
dihydrofolate + deoxy-5-methylcytidylate
show the reaction diagram
5,10-methylenetetrahydrofolate + dCTP
dihydrofolate + deoxy-5-methylcytidine 5'-triphosphate
show the reaction diagram
-
low activity
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5,10-methylenetetrahydrofolate + dCMP
dihydrofolate + deoxy-5-methylcytidylate
show the reaction diagram
-
-
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
25 mM, slight stimulation
Cu2+
-
below 1 mM, stimulation
K+
-
150 mM, stimulation
Mn2+
-
has a similar effect in stimulation but not as strong as Mg2+
Zn2+
-
below 1 mM, stimulation
additional information
-
among the buffers tested, 6-morpholinoethane sulfonate/Mg2+ is the best
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
above 1 mM
Mg2+
-
above 50 mM
Zn2+
-
above 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0313
dCMP
-
-
0.0714
tetrahydrofolic acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
3 min, 90% loss of activity
70
-
3 min, complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, stable for at least 2 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
phage Xp12-infected, partial
-