Information on EC 2.1.1.47 - indolepyruvate C-methyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces griseus

EC NUMBER
COMMENTARY hide
2.1.1.47
-
RECOMMENDED NAME
GeneOntology No.
indolepyruvate C-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + (indol-3-yl)pyruvate = S-adenosyl-L-homocysteine + (S)-3-(indol-3-yl)-2-oxobutanoate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
indolmycin biosynthesis
-
-
Tryptophan metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine: (indol-3-yl)pyruvate C-methyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
54576-88-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + indolepyruvate
?
show the reaction diagram
-
reaction in indolmycin biosynthesis
-
-
?
S-adenosyl-L-methionine + indolepyruvate
S-adenosyl-L-homocysteine + (S)-beta-methylindolepyruvate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + indolepyruvate
?
show the reaction diagram
-
reaction in indolmycin biosynthesis
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no requirement for Mg2+, Mn2+, Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
2,2'-bipyridyl
-
-
iodoacetamide
-
weak
iodoacetic acid
-
weak
N-ethylmaleimide
-
-
p-chloromercuribenzoate
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0048
Indolepyruvate
-
-
0.013
S-adenosyl-L-methionine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
phosphate buffer
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
-
gel filtration
58500
-
sucrose density gradient centrifugation
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
and below, irreversible inactivation
441311, 441312
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
heating inactivates
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing inactivates
-
heating inactivates
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
2°C, up to 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE