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S-adenosyl-L-methionine + guanine46 in methyl deficient tRNA from E. coli
?
-
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAAla/UGC
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAAla/UGC
S-adenosyl-L-methionine + guanine46 in RNAArg/ACG
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAArg/ACG
S-adenosyl-L-methionine + guanine46 in RNAArg/CCG
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAArg/CCG
S-adenosyl-L-methionine + guanine46 in RNAArg/CCU
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAArg/CCU
S-adenosyl-L-methionine + guanine46 in RNAArg/UCU
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAArg/UCU
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAAsn/GUU
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAAsn/GUU
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAAsp/GUC
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAAsp/GUC
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAGly/GCC
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAGly/GCC
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAHis/GUG
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAHis/GUG
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAIle/GAU
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAIle/GAU
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNALys/UUU
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNALys/UUU
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAThr/CGU
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAThr/CGU
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAVal/GAC
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAVal/GAC
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
S-adenosyl-L-methionine + guanine46 in tRNAAla/GGC
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAAla/GGC
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNAMet/CAU
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAMet/CAU
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNAPhe
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAPhe
substrate tRNAPhe of Saccharomyces cerevisiae
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNAPhe/GAA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAPhe/GAA
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNAPro/CGG
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAPro/CGG
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNAPro/GGG
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAPro/GGG
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNAPro/UGG
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAPro/UGG
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNAThr/GGU
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAThr/GGU
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNAThr/UGU
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAThr/UGU
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNATrp/CCA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNATrp/CCA
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNAVal/UAC
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAVal/UAC
-
-
-
?
S-adenosyl-L-methionine + guanine46 in undermethylated tRNA
?
-
-
-
-
?
additional information
?
-
S-adenosyl-L-methionine + guanine46 in RNAAla/UGC
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAAla/UGC
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAAla/UGC
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAAla/UGC
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAArg/ACG
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAArg/ACG
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAArg/ACG
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAArg/ACG
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAArg/CCG
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAArg/CCG
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAArg/CCG
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAArg/CCG
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAArg/CCU
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAArg/CCU
-
-
-
?
S-adenosyl-L-methionine + guanine46 in RNAArg/CCU
S-adenosyl-L-homocysteine + N7-methylguanine46 in RNAArg/CCU
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
-
ir
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
highly specific for tRNA and S-adenosyl-L-methionine, cannot be replaced by methionine plus ATP, formaldehyde plus tetrahydrofolic acid, methylcobalamin and methyltetrahydrofolic acid
-
ir
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptors: methyl-deficient bulk tRNA from E. coli K12met-rel-, tRNAfMet from Bacillus subtilis, formation of 0.8 mol N7-methylguanine residues per mol Bacillus subtilis tRNA, enzyme recognizes and methylates specifically the extra arm unpaired methyl-deficient guanylate residue
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
extend of methylation is directly proportional to the amount of tRNA added, 0.19% of nucleotide residues are methylated
-
ir
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptors: methyl-deficient bulk tRNA from E. coli K12met-rel-, tRNAfMet from Bacillus subtilis, formation of 0.8 mol N7-methylguanine residues per mol Bacillus subtilis tRNA, enzyme recognizes and methylates specifically the extra arm unpaired methyl-deficient guanylate residue
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptor: yeast pre-tRNAPhe
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptor: yeast tRNA1Ala, methylates G located in the extra loop at position 46 from the 5 end of tRNA1Ala
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptor: yeast tRNA1Ala, methylates G located in the extra loop at position 46 from the 5 end of tRNA1Ala
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptors: tRNACys, tRNATrp, tRNAPro, tRNAMet, tRNAMet-i and 2 of each tRNAPhe, tRNAVal and tRNALys, methylation at G46 in the extra loop
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptor: yeast pre-tRNAPhe
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, tRNA site G46 is methylated in vivo
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptor: methyl-deficient tRNA from E. coli
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptor: undermethylated tRNA
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
methyl group acceptor: undermethylated tRNA from Salmonella typhimurium CS 14, NH2- and COOH-group of S-adenosyl-L-methionine as well as the length of hydrocarbon chain connecting them to the aromatic ring of adenosine, chirality at the alpha-C atom and NH2-group of adenosine are important for substrate binding to enzyme
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
tRNA modifying enzyme
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
specific enzyme involved in the maturation of tRNA
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
-
-
-
?
additional information
?
-
no substrates: tRNALeu/CAG, tRNALeu/GAG, and tRNASec(p)/UCA
-
-
-
additional information
?
-
no substrates: tRNALeu/CAG, tRNALeu/GAG, and tRNASec(p)/UCA
-
-
-
additional information
additional information
-
-
not as methyl group acceptors: methyl-deficient DNA from E. coli, polyadenylic acid, polycytidylic acid, polyuridylic acid, polyinosinic acid, copolymers of adenylic acid and uridylic acid and of guanylic acid and cytidylic acid, tobacco mosaic viral RNA, turnip yellow mosaic viral RNA, RNA from bacteriophage f2, CMP, AMP, UMP, GMP, rRNA
-
-
?
additional information
additional information
-
-
methyl group transfer at the polynucleotide level of tRNA, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species
-
-
?
additional information
additional information
-
-
methyl group transfer at the polynucleotide level of tRNA, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species
-
-
?
additional information
additional information
-
-
proteins METTL1 and WDR4 are required for m7G-methyltransferase activity
-
-
?
additional information
additional information
-
-
complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, but Trm82 is not an absolute requirement for activity in vitro, Trm8 may be the catalytically active subunit
-
-
?
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0.00025 - 0.0037
guanine46 in tRNA
-
0.21
methyl deficient tRNA from E. coli
-
-
-
0.0004 - 0.053
S-adenosyl-L-methionine
0.0034
undermethylated tRNA
-
-
-
0.00025
guanine46 in tRNA
pH 7.5, 30°C, tRNA variant with substitution of C48 by A
-
0.00035
guanine46 in tRNA
pH 7.5, 30°C, tRNA variant with disruption of tertiary base pairs between D- and T-arms
-
0.0005
guanine46 in tRNA
pH 7.5, 30°C, tRNA variant with disruption of C13-G22-G46 tertiary base pair
-
0.0014
guanine46 in tRNA
pH 7.5, 30°C, tRNA variant with deletion of the anticodon-arm
-
0.0015
guanine46 in tRNA
pH 7.5, 30°C, RNA
-
0.0037
guanine46 in tRNA
pH 7.5, 30°C, tRNA variant with deletion of the aminoacyl-stem
-
0.0004
S-adenosyl-L-methionine
mutant D97A, 55°C, pH not specified in the publication
0.0007
S-adenosyl-L-methionine
wild-type, 55°C, pH not specified in the publication
0.0008
S-adenosyl-L-methionine
mutant D74A, 55°C, pH not specified in the publication
0.001
S-adenosyl-L-methionine
mutant T132S, 55°C, pH not specified in the publication
0.0017
S-adenosyl-L-methionine
mutant T132A, 55°C, pH not specified in the publication
0.0025
S-adenosyl-L-methionine
mutant H104A, 55°C, pH not specified in the publication
0.0025
S-adenosyl-L-methionine
mutant R107A, 55°C, pH not specified in the publication
0.003
S-adenosyl-L-methionine
mutant K101A, 55°C, pH not specified in the publication
0.003
S-adenosyl-L-methionine
mutant T132C, 55°C, pH not specified in the publication
0.005
S-adenosyl-L-methionine
mutant T165A, 55°C, pH not specified in the publication
0.006
S-adenosyl-L-methionine
mutant Y167F, 55°C, pH not specified in the publication
0.007
S-adenosyl-L-methionine
mutant E168A, 55°C, pH not specified in the publication
0.007
S-adenosyl-L-methionine
mutant E47A, 55°C, pH not specified in the publication
0.0075
S-adenosyl-L-methionine
mutant Y95A, 55°C, pH not specified in the publication
0.01
S-adenosyl-L-methionine
mutant R108A, 55°C, pH not specified in the publication
0.016
S-adenosyl-L-methionine
-
-
0.019
S-adenosyl-L-methionine
mutant Y167A, 55°C, pH not specified in the publication
0.053
S-adenosyl-L-methionine
-
-
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evolution
-
nonessential tRNA modifications by methyltransferases are evolutionarily conserved and have been reported to stabilize mature tRNA molecules and prevent rapid tRNA decay. The tRNA modifying enzymes, NSUN2 and METTL1, are mammalian orthologues of yeast Trm4 and Trm8, which are required for protecting tRNA against tRNA decay
malfunction
absence of m7G46 results in temperature-sensitive growth
malfunction
-
protein synthesis in DELTAtrmB cells is depressed above 70°C. At 80°C, the DtrmB strain exhibits a severe growth defect
malfunction
-
combined knockdown of NSUN2, EC 2.1.1.203, and METTL1 in HeLa cells drastically potentiate sensitivity of cells to 5-fluorouracil, but does not affect cisplatin- and paclitaxel-induced cytotoxicity, synergistic effects of NSUN2 and METTL1 double knockdown, which causes rapid tRNA(ValAAC) degradation induced by destabilizing 5-fluorouracil
physiological function
-
N7 cap methylation is essential for viral replication
physiological function
-
N7-MTase plays an important role in SARS-coronavirus replication/transcription
physiological function
-
the m7G46 modification catalyzed by TrmB may act as one of the key factors in tRNA modification network
physiological function
long C-terminal region of the thermophilic TrmB protein is involved in the protein stability but does not function in the RNA recognition
physiological function
m7G46 methyltransferase Trm8p/Trm82p acts as a hub of synthetic interactions with several tRNA modification enzymes, resulting in temperature-sensitive growth
physiological function
-
the m7G46 modification is required for cell viability at high temperatures via a tRNA modification network. The m7G46 modification induces nearly full modification of several modified nucleotides such as Gm18 and m1G37
physiological function
Trm8p is required for m7G modification. Trm82p is required to maintain cellular levels of Trm8p and to stabilize Trm8p in an active conformation
physiological function
-
Nonessential tRNA modifications by methyltransferases are evolutionarily conserved and have been reported to stabilize mature tRNA molecules and prevent rapid tRNA decay. The tRNA modifying enzyme METTL1 is a mammalian orthologues of yeast Trm8, which is required for protecting tRNA against tRNA decay
physiological function
residues Glu47, Tyr95, Arg108, Thr165 and Tyr167 residues are important for AdoMet binding and Asp74, Asp97, and Thr132 are important for the methyltransfer reaction. During the reaction, the carboxyl group in Asp133 captures the proton of N-H in the guanine base. Thr132 may assist the interaction by formation of a hydrogen bond between the OH group in Thr132 and the O6 atom in the guanine base. Interactions between Thr132, Asp133 and tRNA decide the direction of the guanine base and the N7 atom in G46 causes the nucleophilic attack against the activated methyl group in the bound AdoMet
physiological function
the abundance of m7G in the tRNA extracted from trmB complemented strain is about 50% lower than the abundance of m7G extracted from the wild type strain. Pseudomonas aeruginosa shows a tRNA modification-mediated translational response to H2O2. Loss of TrmB has a strong negative effect on the translation of Phe- and Asp-enriched mRNAs. The TrmB-mediated m7G modification modulates the expression of the catalase genes KatA and KatB, which are enriched with Phe/Asp codons at the translational level. In response to H2O2 exposure, the level of m7G modification increases, consistent with the increased translation efficiency of Phe- and Asp-enriched mRNAs. Inactivation of TrmB leads to decreased KatA and KatB protein abundance and decreased catalase activity, resulting in H2O2-sensitive phenotype
physiological function
-
the abundance of m7G in the tRNA extracted from trmB complemented strain is about 50% lower than the abundance of m7G extracted from the wild type strain. Pseudomonas aeruginosa shows a tRNA modification-mediated translational response to H2O2. Loss of TrmB has a strong negative effect on the translation of Phe- and Asp-enriched mRNAs. The TrmB-mediated m7G modification modulates the expression of the catalase genes KatA and KatB, which are enriched with Phe/Asp codons at the translational level. In response to H2O2 exposure, the level of m7G modification increases, consistent with the increased translation efficiency of Phe- and Asp-enriched mRNAs. Inactivation of TrmB leads to decreased KatA and KatB protein abundance and decreased catalase activity, resulting in H2O2-sensitive phenotype
-
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Bjrk, G.R.; Svensson, I.
Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae
Eur. J. Biochem.
9
207-215
1969
Saccharomyces cerevisiae, Saccharomyces cerevisiae D84
brenda
Hurwitz, J.; Gold, M.; Anders, M.
The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. III. Purification of soluble ribonucleic acid-methylating enzymes
J. Biol. Chem.
239
3462-3473
1964
Escherichia coli
brenda
Hurwitz, J.; Gold, M.; Anders, M.
The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. IV. The properties of the soluble ribonucleic acid-methylating enzymes
J. Biol. Chem.
239
3474-3482
1964
Escherichia coli
brenda
Cimino, F.; Traboni, C.; Colonna, A.; Izzo, P.; Salvatore, F.
Purification and properties of several transfer RNA methyltransferases from S. typhimurium
Mol. Cell. Biochem.
36
95-104
1981
Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Colonna, A.; Ciliberto, G.; Santamaria, R.; Cimino, F.; Salvatore, F.
Isolation and characterization of a tRNA(guanine-7-)-methyltransferase from Salmonella typhimurium
Mol. Cell. Biochem.
52
97-106
1983
Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Dubois, E.G.; Weil, J.H.
A 7-methylguanine tRNA methylase present in crown-gall cells but absent in normal Parthenocissus tricuspidata L. cells
Plant Sci. Lett.
8
385-394
1977
Parthenocissus tricuspidata
-
brenda
Dubois, E.G.; Dirheimer, G.; Weil, J.H.
Characterization of an organelle-specific 7-methylguanine tRNA methylase in the chloroplasts and mitochondria of Phaseolus vulgaris
Plant Sci. Lett.
5
17-26
1975
Phaseolus vulgaris
-
brenda
Aschhoff, H.J.; Elten, H.; Arnold, H.H.; Mahal, G.; Kersten, W.; Kersten, H.
7-Methylguanine specific tRNA-methyltransferase from Escherichia coli
Nucleic Acids Res.
3
3109-3122
1976
Escherichia coli, Escherichia coli MRE 600
brenda
Paolella, G.; Ciliberto, G.; Traboni, C.; Cimino, F.; Salvatore, F.
Effect of adenosylhomocysteine and other analog thioethers on a prokaryotic tRNA (guanine-7)-methyltransferase
Arch. Biochem. Biophys.
219
149-154
1982
Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Alexandrov, A.; Martzen, M.R.; Phizicky, E.M.
Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA
RNA
8
1253-1266
2002
Saccharomyces cerevisiae, Homo sapiens
brenda
Matsumoto, K.; Toyooka, T.; Tomikawa, C.; Ochi, A.; Takano, Y.; Takayanagi, N.; Endo, Y.; Hori, H.
RNA recognition mechanism of eukaryote tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex)
FEBS Lett.
581
1599-1604
2007
Saccharomyces cerevisiae (Q03774 and Q12009), Saccharomyces cerevisiae
brenda
Alexandrov, A.; Chernyakov, I.; Gu, W.; Hiley, S.L.; Hughes, T.R.; Grayhack, E.J.; Phizicky, E.M.
Rapid tRNA decay can result from lack of nonessential modifications
Mol. Cell
21
87-96
2006
Saccharomyces cerevisiae (Q03774 and Q12009)
brenda
Zegers, I.; Gigot, D.; van Vliet, F.; Tricot, C.; Aymerich, S.; Bujnicki, J.M.; Kosinski, J.; Droogmans, L.
Crystal structure of Bacillus subtilis TrmB, the tRNA (m7G46) methyltransferase
Nucleic Acids Res.
34
1925-1934
2006
Bacillus subtilis (O34522), Bacillus subtilis
brenda
Tomikawa, C.; Hori, H.
The core domain of Aquifex aeolicus tRNA (m7G46) methyltransferase has the methyl-transfer activity to tRNA
Nucleic Acids Symp. Ser.
50
245-246
2006
Aquifex aeolicus (O66479), Aquifex aeolicus
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