Information on EC 2.1.1.325 - juvenile hormone-III synthase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.325
-
RECOMMENDED NAME
GeneOntology No.
juvenile hormone-III synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + (2E,6E)-farnesoate = S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
show the reaction diagram
(1)
-
-
-
S-adenosyl-L-methionine + juvenile hormone III acid = S-adenosyl-L-homocysteine + juvenile hormone III
show the reaction diagram
(2)
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Insect hormone biosynthesis
-
-
juvenile hormone III biosynthesis I
-
-
juvenile hormone III biosynthesis II
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:(2E,6E)-farnesoate O-methyltransferase
The enzyme, found in insects, is involved in the synthesis of juvenile hormone III, a sesquiterpenoid that regulates several processes including embryonic development, metamorphosis, and reproduction, in many insect species.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
seabuckthorn carpenterworm
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
inhibition of enzyme transcription leads to inhibition of egg maturation and larval development
metabolism
-
the enzyme has little if any effect on sesquiterpenoid biosynthesis
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (2E,6E)-farnesoate
S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
show the reaction diagram
S-adenosyl-L-methionine + (2E,6Z)-10cis-juvenile hormone I acid
S-adenosyl-L-homocysteine + juvenile hormone I
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + (2Z,6E)-10cis-juvenile hormone I acid
S-adenosyl-L-homocysteine + juvenile hormone I
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + (cis/trans/cis)-juvenile hormone I acid
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + (cis/trans/trans)-juvenile hormone I acid
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + (trans/cis/cis)-juvenile hormone I acid
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + homofarnesoate
S-adenosyl-L-homocysteine + methylhomofarnesoate
show the reaction diagram
S-adenosyl-L-methionine + juvenile hormone I acid
S-adenosyl-L-homocysteine + juvenile hormone I
show the reaction diagram
highest activity
-
-
?
S-adenosyl-L-methionine + juvenile hormone I acid bisepoxide
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + juvenile hormone II acid
S-adenosyl-L-homocysteine + juvenile hormone II
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + juvenile hormone III acid
S-adenosyl-L-homocysteine + juvenile hormone III
show the reaction diagram
S-adenosyl-L-methionine + juvenile hormone III acid bisepoxide
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + laurate
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + lauric acid
S-adenosyl-L-homocysteine + ?
show the reaction diagram
S-adenosyl-L-methionine + palmitate
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + palmitic acid
S-adenosyl-L-homocysteine + ?
show the reaction diagram
additional information
?
-
no activity with stearic acid, lauric acid, tridecanoic acid, linoleic acid, linoleidic acid, and linolenic acid
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (2E,6E)-farnesoate
S-adenosyl-L-homocysteine + methyl (2E,6E)-farnesoate
show the reaction diagram
S-adenosyl-L-methionine + juvenile hormone III acid
S-adenosyl-L-homocysteine + juvenile hormone III
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00046 - 0.00223
(2E,6E)-farnesoate
0.00458
(2E,6Z)-10cis-juvenile hormone I acid
at pH 7.9 and 25C
-
0.00565
(2Z,6E)-10cis-juvenile hormone I acid
at pH 7.9 and 25C
-
0.00387
(cis/trans/cis)-juvenile hormone I acid
at pH 7.9 and 25C
-
0.01027
(cis/trans/trans)-juvenile hormone I acid
at pH 7.9 and 25C
-
0.000008
(trans/cis/cis)-juvenile hormone I acid
at pH 7.9 and 25C
-
0.00002 - 0.01997
homofarnesoate
-
0.00028
juvenile hormone I acid bisepoxide
at pH 7.9 and 25C
-
0.00253 - 0.01468
juvenile hormone III acid
0.00016
juvenile hormone III acid bisepoxide
at pH 7.9 and 25C
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000217 - 0.168
(2E,6E)-farnesoate
0.000003
(2E,6Z)-10cis-juvenile hormone I acid
Diaphorina citri
A0A075W3Z0
at pH 7.9 and 25C
-
0.000008
(2Z,6E)-10cis-juvenile hormone I acid
Diaphorina citri
A0A075W3Z0
at pH 7.9 and 25C
-
0.000245
(cis/trans/cis)-juvenile hormone I acid
Aedes aegypti
Q206L4
at pH 7.9 and 25C
-
0.000875
(cis/trans/trans)-juvenile hormone I acid
Aedes aegypti
Q206L4
at pH 7.9 and 25C
-
0.000006
(trans/cis/cis)-juvenile hormone I acid
Aedes aegypti
Q206L4
at pH 7.9 and 25C
-
0.00016 - 0.00022
homofarnesoate
-
0.000011
juvenile hormone I acid bisepoxide
Aedes aegypti
Q206L4
at pH 7.9 and 25C
-
0.000013 - 0.128
juvenile hormone III acid
0.00001
juvenile hormone III acid bisepoxide
Aedes aegypti
Q206L4
at pH 7.9 and 25C
-
0.0017
laurate
Drosophila melanogaster
-
at pH 7.5 and 25C
0.000683
palmitate
Drosophila melanogaster
-
at pH 7.5 and 25C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.09716 - 1.645
(2E,6E)-farnesoate
41811
0.00066
(2E,6Z)-10cis-juvenile hormone I acid
Diaphorina citri
A0A075W3Z0
at pH 7.9 and 25C
213992
0.1434
(2Z,6E)-10cis-juvenile hormone I acid
Diaphorina citri
A0A075W3Z0
at pH 7.9 and 25C
213991
0.06335
(cis/trans/cis)-juvenile hormone I acid
Aedes aegypti
Q206L4
at pH 7.9 and 25C
213994
0.08523
(cis/trans/trans)-juvenile hormone I acid
Aedes aegypti
Q206L4
at pH 7.9 and 25C
213993
0.7708
(trans/cis/cis)-juvenile hormone I acid
Aedes aegypti
Q206L4
at pH 7.9 and 25C
213997
0.00803 - 10.85
homofarnesoate
213990
0.03929
juvenile hormone I acid bisepoxide
Aedes aegypti
Q206L4
at pH 7.9 and 25C
213995
0.00514 - 0.0782
juvenile hormone III acid
120914
0.06146
juvenile hormone III acid bisepoxide
Aedes aegypti
Q206L4
at pH 7.9 and 25C
213996
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
calculated from amino acid sequence
4.6
-
estimated from amino acid sequence
4.7
calculated from amino acid sequence
5.9
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
highest expression
Manually annotated by BRENDA team
additional information
-
marginal expression levels in prothoracic gland, midgut, epidermis, fat body, Malpighian tubules, and subesophageal ganglion
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-Talon column chromatography
HiTrap chelating column chromatography
-
HiTrap column chromatography
HiTrap Ni-chelating column chromatography, and gel filtration
-
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography, and gel filtration
Ni2+ affinity column chromatography and Sephacryl S-300 gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli One Shot BL21 (DE3) cells
expressed in Pichia pastoris
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme activity is increases about 18fold 14 days after eyestalk ablation
-
enzyme expression significantly increases in the late-vitellogenic stage and mature stage of ovarian development
-
enzyme transcription is suppressed during metamorphosis
-
in females, the enzyme expression levels are significantly downregulated in eyestalk during ovarian development
in females, the enzyme expression levels are significantly up-regulated in thoracic ganglion during ovarian development, while in males expression levels increase in both thoracic ganglion and eyestalk during testicular development stages
in ovarian development, the mRNA level of the enzyme remains low in stage I and II, then increases considerably to a maximum in stage III, and declines slightly in stage IV
in the presence of S-benzyl-O-ethyl phosphoramidothiolate, homogenates of 0-day corpora allata show high levels of enzyme activity
-
mRNA expression is upregulated 6 h after Vibrio anguillarum challenge treatment in hemocytes and 12 h after Vibrio anguillarum challenge treatment in hepatopancreas
the enzyme is expressed specifically in the corpora allata throughout the third and fourth instar
the enzyme is undetectable in the pupal stage and during subsequent adult development, but increases again in adults by day 7
the enzyme's mRNA level gradually declines during larval development, and the lowest amount of expression is observed in the pupal stage, while it increases to a higher level during adult stages
-
the genes Tkv and Mad upregulate transcription and activity of the enzyme
-
transcript levels are high in early 4th instar larvae, undetectable in early and 1-day-old pupae, and rapidly increase 44-46 h after pupation to relatively high levels at adult eclosion (approximately 48-50 h after pupation)
-
transcript levels of the enzyme are not up-regulated by 20-hydroxyecdysone
-
treatment with mandibular organ-inhibiting hormone leads to a significant decline in enzyme activity and mRNA levels after 5h
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G131V
-
the mutation is significantly associated with greater body weight and carapace width