Information on EC 2.1.1.313 - 25S rRNA (uracil2634-N3)-methyltransferase

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY hide
2.1.1.313
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RECOMMENDED NAME
GeneOntology No.
25S rRNA (uracil2634-N3)-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + uracil2634 in 25S rRNA = S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (uracil2634-N3)-methyltransferase
The enzyme, described from the yeast Saccharomyces cerevisiae, is involved in ribosome biogenesis.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uracil2634 in 25S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uracil2634 in 25S rRNA
S-adenosyl-L-homocysteine + N3-methyluracil2634 in 25S rRNA
show the reaction diagram
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tagged enzyme is purified by Nickel-NTA metal affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
plasmid pSH26 carrying N terminal 6xHis-Smt3tagged Bmt5, is constructed using pPK591 by gap repair. For overexpression, BL21 (DE3) cells are transformed with isolated plasmids and grown in LB medium containing 100 mg/ml ampicillin at 37C to an OD600 of 0.6