Information on EC 2.1.1.302 - 3-hydroxy-5-methyl-1-naphthoate 3-O-methyltransferase

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The expected taxonomic range for this enzyme is: Streptomyces sahachiroi

EC NUMBER
COMMENTARY hide
2.1.1.302
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RECOMMENDED NAME
GeneOntology No.
3-hydroxy-5-methyl-1-naphthoate 3-O-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 3-hydroxy-5-methyl-1-naphthoate = S-adenosyl-L-homocysteine + 3-methoxy-5-methyl-1-naphthoate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:3-hydroxy-5-methyl-1-naphthoate 3-O-methyltransferase
The enzyme from the bacterium Streptomyces sahachiroi is involved in the biosynthesis of 3-methoxy-5-methyl-1-naphthoate, a component of of the the antitumor antibiotic azinomycin B.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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the enzyme is involved in biosynthesis of 3-methoxy-5-methyl naphthoate a component of of the the antitumor antibiotic azinomycin B
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3-hydroxy-5-methylnaphthoate
S-adenosyl-L-homocysteine + 3-methoxy-5-methylnaphthoate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3-hydroxy-5-methylnaphthoate
S-adenosyl-L-homocysteine + 3-methoxy-5-methylnaphthoate
show the reaction diagram
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the enzyme is involved in biosynthesis of 3-methoxy-5-methyl naphthoate a component of of the the antitumor antibiotic azinomycin B
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-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
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5 mM, about 15% inhibition
Fe2+
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5 mM, about 20% inhibition
Mg2+
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5 mM, about 5% inhibition
Mn2+
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5 mM, about 15% inhibition
Zn2+
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5 mM, about 45% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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5 mM, 10% increase inactivity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.145
3-hydroxy-5-methylnaphthoate
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pH 8.0, 25°C
0.061
S-adenosyl-L-methionine
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pH 8.0, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0073
3-hydroxy-5-methylnaphthoate
Streptomyces sahachiroi
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pH 8.0, 25°C
0.0063
S-adenosyl-L-methionine
Streptomyces sahachiroi
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pH 8.0, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
3-hydroxy-5-methylnaphthoate
Streptomyces sahachiroi
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pH 8.0, 25°C
29153
0.1
S-adenosyl-L-methionine
Streptomyces sahachiroi
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pH 8.0, 25°C
24
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 10
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pH 7.5: about 50% of maximal activity, pH 10.0: about 55% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Streptomyces albus or in Escherichia coli Bö21(DE3)
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