Information on EC 2.1.1.293 - 6-hydroxytryprostatin B O-methyltransferase

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The expected taxonomic range for this enzyme is: Aspergillus fumigatus

EC NUMBER
COMMENTARY hide
2.1.1.293
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RECOMMENDED NAME
GeneOntology No.
6-hydroxytryprostatin B O-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 6-hydroxytryprostatin B = S-adenosyl-L-homocysteine + tryprostatin A
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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fumitremorgin C biosynthesis
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Staurosporine biosynthesis
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fumitremorgin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine + 6-hydroxytryprostatin B = S-adenosyl-L-homocysteine + tryprostatin A
Involved in the biosynthetic pathways of several indole alkaloids such as tryprostatins, fumitremorgins and verruculogen.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
all ftm genes are expressed almost equally well in both Af293 and BM939 strains. But a point mutation found in ftmD in the genome of Af293 causes an arginine to leucine substitution at position 202 of FtmD, resulting in a dramatic decrease of the catalytic efficiency of FtmD. This mutated form of FtmD appeared not functioning under physiological conditions in Af293 to produce any detectable levels of TPS-A or any downstream metabolites
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 6-hydroxytryprostatin B
S-adenosyl-L-homocysteine + tryprostatin A
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 6-hydroxytryprostatin B
S-adenosyl-L-homocysteine + tryprostatin A
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021 - 0.12
6-hydroxytryprostatin B
0.0045 - 0.011
S-adenosyl-L-methionine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8.2 - 27
6-hydroxytryprostatin B
2 - 8
S-adenosyl-L-methionine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
66 - 1300
6-hydroxytryprostatin B
10404
1500 - 2500
S-adenosyl-L-methionine
24
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
the wild type enzyme shows about 42% activity at pH 6.0, about 78% activity at pH 6.5, and about 90% activity at pH 7.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 40
the wild type enzyme shows 100% activity at 10C, about 95% activity at 20C, about 75% activity at 30C, about 35% activity at 35C, and no activity at 40C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64000
2 * 64000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Talon-Co2+ column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
gene ftmD, encoded in the ftm gene cluster, recombinant expression in Escherichia coli strain BL21(DE3)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
engineered production of tryprostatins in Escherichia coli through reconstitution of a partial ftm biosynthetic gene cluster from Aspergillus sp., i.e. four genes, ftmABCD, on two compatible plasmids