Information on EC 2.1.1.277 - anthranilate O-methyltransferase

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The expected taxonomic range for this enzyme is: Zea mays

EC NUMBER
COMMENTARY hide
2.1.1.277
-
RECOMMENDED NAME
GeneOntology No.
anthranilate O-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + anthranilate = S-adenosyl-L-homocysteine + O-methyl anthranilate
show the reaction diagram
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:anthranilate O-methyltransferase
In the plant maize (Zea mays), the isoforms AAMT1 and AAMT2 are specific for anthranilate while AAMT3 also has the activity of EC 2.1.1.273, benzoate methyltransferase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
isoenzyme AAMT1 is responsible for most of the S-adenosyl-L-methionine-dependent methyltransferase activity and methyl anthranilate formation observed in maize after herbivore damage
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + anthranilate
S-adenosyl-L-homocysteine + O-methyl anthranilate
show the reaction diagram
S-adenosyl-L-methionine + benzoate
S-adenosyl-L-homocysteine + methyl benzoate
show the reaction diagram
26% of the activity with anthanilate, isoenzyme AAMT3
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + anthranilate
S-adenosyl-L-homocysteine + O-methyl anthranilate
show the reaction diagram
B6SU46, D9J100
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-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.311 - 0.641
anthranilate
0.076 - 0.094
S-adenosyl-L-methionine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.37 - 0.45
anthranilate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7 - 1.2
anthranilate
474
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned and expressed with an N-terminal His tag in Escherichia coli; cloned and expressed with an N-terminal His tag in Escherichia coli; cloned and expressed with an N-terminal His tag in Escherichia coli