Information on EC 2.1.1.276 - gibberellin A4 carboxyl methyltransferase

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The expected taxonomic range for this enzyme is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
2.1.1.276
-
RECOMMENDED NAME
GeneOntology No.
gibberellin A4 carboxyl methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + gibberellin A4 = S-adenosyl-L-homocysteine + methyl gibberellin A4
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
gibberellin inactivation II (methylation)
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:gibberellin A4 O-methyltransferase
The enzyme also methylates gibberellins A34 (80%), A9 (60%), and A3 (45%) with significant activity.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + gibberellin A1
S-adenosyl-L-homocysteine + methyl gibberellin A1
show the reaction diagram
30% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A20
S-adenosyl-L-homocysteine + methyl gibberellin A20
show the reaction diagram
15% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A3
O-methyl gibberellin A3 + S-adenosyl-L-homocysteine
show the reaction diagram
S-adenosyl-L-methionine + gibberellin A3
S-adenosyl-L-homocysteine + methyl gibberellin A3
show the reaction diagram
45% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A34
S-adenosyl-L-homocysteine + methyl gibberellin A34
show the reaction diagram
80% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A4
S-adenosyl-L-homocysteine + methyl gibberellin A4
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + gibberellin A51
S-adenosyl-L-homocysteine + methyl gibberellin A51
show the reaction diagram
16% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A9
S-adenosyl-L-homocysteine + methyl gibberellin A9
show the reaction diagram
60% of the activity compared to methylation of gibberellin A4
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + gibberellin A3
O-methyl gibberellin A3 + S-adenosyl-L-homocysteine
show the reaction diagram
S-adenosyl-L-methionine + gibberellin A4
S-adenosyl-L-homocysteine + methyl gibberellin A4
show the reaction diagram
Q5XF78
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
5 mM, more than 90% inhibition
Fe3+
5 mM, more than 90% inhibition
Zn2+
5 mM, more than 90% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0059
gibberellin a4
pH 8.0, 30C
0.0019
gibberellin a9
pH 8.0, 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0015
gibberellin a4
Arabidopsis thaliana
Q5XF78
pH 8.0, 30C
0.0018
gibberellin a9
Arabidopsis thaliana
Q5XF78
pH 8.0, 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
gibberellin a4
Arabidopsis thaliana
Q5XF78
pH 8.0, 30C
9570
0.9
gibberellin a9
Arabidopsis thaliana
Q5XF78
pH 8.0, 30C
5315
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
low expression
Manually annotated by BRENDA team
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43300
x * 43300, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 43300, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli, expression in Arabidopsis thaliana under the control of the 35S cauliflower mosaic virus promoter
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
during seed germination, the expression of the enzyme decreases until the cotyledons expand out of the seed coat
expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development