Information on EC 2.1.1.275 - gibberellin A9 O-methyltransferase

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The expected taxonomic range for this enzyme is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
2.1.1.275
-
RECOMMENDED NAME
GeneOntology No.
gibberellin A9 O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + gibberellin A9 = S-adenosyl-L-homocysteine + methyl gibberellin A9
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
gibberellin inactivation II (methylation)
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-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:gibberellin A9 O-methyltransferase
The enzyme also methylates gibberellins A20 (95%), A3 (80%), A4 (69%) and A34 (46%) with significant activity.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
GAMT1 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + gibberellin 1
S-adenosyl-L-homocysteine + methyl gibberellin A1
show the reaction diagram
25% of the activity compared to methylation of gibberellin A9
-
-
?
S-adenosyl-L-methionine + gibberellin A19
S-adenosyl-L-homocysteine + methyl gibberellin A19
show the reaction diagram
19% of the activity compared to methylation of gibberellin A9
-
-
?
S-adenosyl-L-methionine + gibberellin A20
S-adenosyl-L-homocysteine + methyl gibberellin A20
show the reaction diagram
95% of the activity compared to methylation of gibberellin A9
-
-
?
S-adenosyl-L-methionine + gibberellin A3
S-adenosyl-L-homocysteine + methyl gibberellin A3
show the reaction diagram
80% of the activity compared to methylation of gibberellin A9
-
-
?
S-adenosyl-L-methionine + gibberellin A34
S-adenosyl-L-homocysteine + methyl gibberellin A34
show the reaction diagram
46% of the activity compared to methylation of gibberellin A9
-
-
?
S-adenosyl-L-methionine + gibberellin A4
S-adenosyl-L-homocysteine + methyl gibberellin A4
show the reaction diagram
69% of the activity compared to methylation of gibberellin A9
-
-
?
S-adenosyl-L-methionine + gibberellin A51
S-adenosyl-L-homocysteine + methyl gibberellin A51
show the reaction diagram
32% of the activity compared to methylation of gibberellin A9
-
-
?
S-adenosyl-L-methionine + gibberellin A9
S-adenosyl-L-homocysteine + methyl gibberellin A9
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + gibberellin A9
S-adenosyl-L-homocysteine + methyl gibberellin A9
show the reaction diagram
F4JUY5
methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
5 mM, 3fold activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
5 mM, more than 90% inhibition
Fe2+
5 mM, more than 90% inhibition
Fe3+
5 mM, more than 90% inhibition
Zn2+
5 mM, more than 90% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NH4+
5 mM, 4fold activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0054
gibberellin a4
pH 8.0, 30C
0.0158
gibberellin a9
pH 8.0, 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
gibberellin a4
Arabidopsis thaliana
F4JUY5
pH 8.0, 30C
0.026
gibberellin a9
Arabidopsis thaliana
F4JUY5
pH 8.0, 30C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.85
gibberellin a4
Arabidopsis thaliana
F4JUY5
pH 8.0, 30C
9570
1.6
gibberellin a9
Arabidopsis thaliana
F4JUY5
pH 8.0, 30C
5315
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GAMT1 expression is localized mostly to seed
Manually annotated by BRENDA team
expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
x * 40000, SDS-PAGE
41500
x * 41500, calculated from sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 40000, SDS-PAGE; x * 41500, calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Solanum lycopersicum
-
expression in Escherichia coli, expression in Arabidopsis under the control of the 35S cauliflower mosaic virus promoter
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development