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Information on EC 2.1.1.272 - cobalt-factor III methyltransferase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Bacillus megaterium
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cobalt-factor III methyltransferase
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S-adenosyl-L-methionine + cobalt-factor III + reduced acceptor = S-adenosyl-L-homocysteine + cobalt-precorrin-4 + acceptor
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vitamin B12 metabolism
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Porphyrin and chlorophyll metabolism
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S-adenosyl-L-methionine:cobalt-factor III 17-methyltransferase (ring contracting)
Isolated from Bacillus megaterium. The enzyme catalyses both methylation at C-17 and ring contraction. Contains a [4Fe-4S] cluster. It can also convert cobalt-precorrin-3 to cobalt-precorrin-4. The reductant may be thioredoxin.
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CbiH60
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gene name
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UniProt
brenda
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S-adenosyl-L-methionine + cobalt(II)-factor-III + dithiothreitol
S-adenosyl-L-homocysteine + cobalt(II)-precorrin-4 + oxidized dithiothreitol
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S-adenosyl-L-methionine + cobalt(II)-precorrin-3
S-adenosyl-L-homocysteine + cobalt(II)-factor-IV
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additional information
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additional information
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enzyme catalyzes both methylation at C-17 and ring contraction
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additional information
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enzyme catalyzes both methylation at C-17 and ring contraction
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[4Fe-4S]-center
enzyme contains a [4Fe-4S]-center required for ring contraction reaction. Iron content is 4.8 mol of iron/mol of enzyme
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65000
x * 65000, SDS-PAGE of recombinant enzyme including His-tag
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x * 65000, SDS-PAGE of recombinant enzyme including His-tag
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expression in Bacillus megaterium DSM319
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C402A
mutation introduced to disrupt the iron-sulfur center. Mutant lacks any visible coloration and has decreased absorbance between 350 and 420 nm and less than 95% of wild-type activity
C443A
mutation introduced to disrupt the iron-sulfur center. Mutant lacks any visible coloration and has decreased absorbance between 350 and 420 nm and less than 95% of wild-type activity
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CBIH_BACME
540
59510
Swiss-Prot
A0A0U5H4D9_9EURY
343
35767
TrEMBL
D4GP60_HALVD
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
294
31843
TrEMBL
M1XTX4_NATM8
Natronomonas moolapensis (strain DSM 18674 / JCM 14361 / 8.8.11)
308
33055
TrEMBL
Q18FW8_HALWD
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
286
31004
TrEMBL
Q18FW9_HALWD
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
355
37278
TrEMBL
B0R5V7_HALS3
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
276
29666
TrEMBL
G0LMI5_HALWC
Haloquadratum walsbyi (strain DSM 16854 / JCM 12705 / C23)
286
31004
TrEMBL
G0LMI4_HALWC
Haloquadratum walsbyi (strain DSM 16854 / JCM 12705 / C23)
355
37236
TrEMBL
A0A1U7EUK6_NATPD
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara)
337
35527
TrEMBL
M1Y5P9_NATM8
Natronomonas moolapensis (strain DSM 18674 / JCM 14361 / 8.8.11)
372
38519
TrEMBL
A0A1U7EUK2_NATPD
Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / NBRC 14720 / NCIMB 2260 / Gabara)
283
30732
TrEMBL
D4GP59_HALVD
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
344
36370
TrEMBL
A0A1Y0HL32_9PROT
242
26880
TrEMBL
A0A140LED1_9CLOT
246
27222
TrEMBL
B0R5V8_HALS3
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
335
35092
TrEMBL
A0A0U5H3D9_9EURY
322
34730
TrEMBL
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Moore, S.J.; Biedendieck, R.; Lawrence, A.D.; Deery, E.; Howard, M.J.; Rigby, S.E.; Warren, M.J.
Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway
J. Biol. Chem.
288
297-305
2013
Bacillus megaterium, Bacillus megaterium (O87689)
brenda
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