Information on EC 2.1.1.257 - tRNA (pseudouridine54-N1)-methyltransferase

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The expected taxonomic range for this enzyme is: Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
2.1.1.257
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RECOMMENDED NAME
GeneOntology No.
tRNA (pseudouridine54-N1)-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + pseudouridine54 in tRNA = S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (pseudouridine54-N1)-methyltransferase
While this archaeal enzyme is specific for the 54 position and does not methylate pseudouridine at position 55, the presence of pseudouridine at position 55 is necessary for the efficient methylation of pseudouridine at position 54 [2,3].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene VNG1980C
UniProt
Manually annotated by BRENDA team
gene VNG1980C
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
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Methanocaldococcus jannaschii TrmY requires C55 (or possibly U55) in the tRNA to produce m1PSI54
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + pseudouridine in 5'-GAUPsiCAACGCC-3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-GAUmethylPsiCAACGCC-3'
show the reaction diagram
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-
-
?
S-adenosyl-L-methionine + pseudouridine in 5'-PsiCAACGCC-3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-methylPsiCAACGCC-3'
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + pseudouridine in 5'-UPsiCAACGC--3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-UmethylPsiCAACGC--3'
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + pseudouridine in 5'-UPsiCAACGCC-3'
S-adenosyl-L-homocysteine + N1-methylpseudouridine in 5'-UmethylPsiCAACGCC-3'
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + pseudouridine54 in elongator tRNAMet
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in elongator tRNAMet
show the reaction diagram
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-
-
?
S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
show the reaction diagram
S-adenosyl-L-methionine + pseudouridine54 in tRNATrp
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNATrp
show the reaction diagram
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-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + pseudouridine54 in tRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-homocysteine
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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Methanocaldococcus jannaschii TrmY requires C55 (or possibly U55) in the tRNA to produce m1PSI54
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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the enzyme shows a single domain structure consisting of eight-stranded beta-sheets sandwiched by six alpha-helices at both sides. Mj1640 has limited structural extension at its N-terminus, which is unique to this family member, structure modeling based on the crystal structure, overview
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant wild-type and selenmethionine-labeled DUF358/Mj1640 in complex with S-adenosyl-L-methionine, at 20°C, mixing of 0.001 ml of protein solution with 0.001 l of reservoir containing 20% PEG 3350, 25 mM NH4F, 20 mM MgCl2, 100 mM Tris, pH 7.5, X-ray diffraction structure determination and analysis at 1.4 A resolution
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged wild-type and selenmethionine-labeled Mj1640 from Escherichia coli strain Bl21(DE3) by nickel affinity chromatography and gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene Mj1640, expression of wild-type and selenmethionine-labeled enzyme as N-terminally His-tagged protein in Escherichia coli strain Bl21(DE3)
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gene trmY or HVO_1989
gene VNG1980C, expression of the gene encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
expression of the gene VNG1980C encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain
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