Information on EC 2.1.1.254 - erythromycin 3''-O-methyltransferase

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The expected taxonomic range for this enzyme is: Saccharopolyspora erythraea

EC NUMBER
COMMENTARY hide
2.1.1.254
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RECOMMENDED NAME
GeneOntology No.
erythromycin 3''-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + erythromycin C = S-adenosyl-L-homocysteine + erythromycin A
show the reaction diagram
S-adenosyl-L-methionine + erythromycin D = S-adenosyl-L-homocysteine + erythromycin B
show the reaction diagram
(2)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of 12-, 14- and 16-membered macrolides
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Biosynthesis of antibiotics
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erythromycin A biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:erythromycin C 3''-O-methyltransferase
The enzyme methylates the 3 position of the mycarosyl moiety of erythromycin C, forming the most active form of the antibiotic, erythromycin A. It can also methylate the precursor erythromycin D, forming erythromycin B, which is then converted to erythromycin A by EC 1.14.13.154, erythromycin 12 hydroxylase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
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the enzyme catalyzes all of the 3''-O-methylation reactions of the erythromycin biosynthetic pathway in Saccharopolyspora erythraea
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + erythromycin C
S-adenosyl-L-homocysteine + erythromycin A
show the reaction diagram
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?
S-adenosyl-L-methionine + erythromycin D
S-adenosyl-L-homocysteine + erythromycin B
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + erythromycin C
S-adenosyl-L-homocysteine + erythromycin A
show the reaction diagram
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?
S-adenosyl-L-methionine + erythromycin D
S-adenosyl-L-homocysteine + erythromycin B
show the reaction diagram
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?
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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introduction of additional eryK and eryG genes into Saccharopolyspora erythraea shows significant impacts on their transcription levels and enhances the biotransformation process from erythromycin D to erythromycin A with gene dose effects. At the eryK/eryG copy number ratio of 3:2 as well as their resultant transcript ratio of around 2.5:1 to 3.0:1, erythromycin B and erythromycin C are nearly completely eliminated and accordingly converted to erythromycin A
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