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S-adenosyl-L-methionine:erythromycin C 3''-O-methyltransferase
The enzyme methylates the 3 position of the mycarosyl moiety of erythromycin C, forming the most active form of the antibiotic, erythromycin A. It can also methylate the precursor erythromycin D, forming erythromycin B, which is then converted to erythromycin A by EC 18.104.22.168, erythromycin 12 hydroxylase.
disruption of the transcription unit, herein designated eryG, by insertion of an integrational plasmid vector, causes a block at the terminal step in the biosynthesis of erythromycin, the conversion of erythromycin C to A by 0-methylation
introduction of additional eryK and eryG genes into Saccharopolyspora erythraea shows significant impacts on their transcription levels and enhances the biotransformation process from erythromycin D to erythromycin A with gene dose effects. At the eryK/eryG copy number ratio of 3:2 as well as their resultant transcript ratio of around 2.5:1 to 3.0:1, erythromycin B and erythromycin C are nearly completely eliminated and accordingly converted to erythromycin A
Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases