Information on EC 2.1.1.250 - trimethylamine-corrinoid protein Co-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.1.1.250
-
RECOMMENDED NAME
GeneOntology No.
trimethylamine-corrinoid protein Co-methyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] = a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Methane metabolism
-
-
methanogenesis from trimethylamine
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
trimethylamine:5-hydroxybenzimidazolylcobamide Co-methyltransferase
The enzyme, which catalyses the transfer of a methyl group from trimethylamine to a trimethylamine-specific corrinoid protein (MttC), is involved in methanogenesis from trimethylamine. The enzyme contains the unusual amino acid pyrrolysine [2]. Methylation of the corrinoid protein requires the central cobalt to be in the Co(I) state. During methylation the cobalt is oxidized to the Co(III) state. The methylated corrinoid protein is substrate for EC 2.1.1.247, methylated methylamine-specific corrinoid protein:coenzyme M methyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
53414-88-3
methylcobalamin-coenzyme M methyltransferase, EC 2.1.1.246 to EC 2.1.1.253
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene DSY3156 or mtgB
UniProt
Manually annotated by BRENDA team
gene DSY3156 or mtgB
UniProt
Manually annotated by BRENDA team
gene mttB
UniProt
Manually annotated by BRENDA team
MttB; gene mttB
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glycine betaine + cob(I)alamin
dimethylglycine + methylcob(III)alamin
show the reaction diagram
trimethylamine + a [Co(I) methylamine-specific corrinoid protein]
a [methyl-Co(III) methylamine-specific corrinoid protein] + dimethylamine
show the reaction diagram
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
a [methyl-Co(III) methylamine-specific corrinoid protein] + dimethylamine
show the reaction diagram
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
trimethylamine + a [Co(I) methylamine-specific corrinoid protein]
a [methyl-Co(III) methylamine-specific corrinoid protein] + dimethylamine
show the reaction diagram
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
a [methyl-Co(III) methylamine-specific corrinoid protein] + dimethylamine
show the reaction diagram
trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
show the reaction diagram
additional information
?
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
RamA
-
a 60-kDa monomeric iron sulfur protein, is a protein required for reductive activation of corrinoid-dependent methylamine methyltransferase reactions in methanogenic archaea, it is required for in vitro ATP-dependent reductive activation of trimethylamine:CoM methyl transfer mediating the ATP-dependent reductive activation of Co(II) corrinoid to the Co(I) state for the trimethylamine corrinoid protein, MttC, overview
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
-
1 * 50000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 50000
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme as the MttB-MttC complex by repeated gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene mttB, co-transcription with gene mtbB1 encoding the DMA methyltransferase. The genes, organized on the chromosome in the order mtbC, mttB, mttC, mttP, and mtbB1, form a single transcriptional unit. The genes of all methyltransferases each contain a single in-frame amber codon
gene mttB, single gene
-
gene mttB1C1, operon encoding TMA methylamine methyltransferase, quantitative RT-PCR expression analysis, comparison with other methylamine methyltransferases, overview. Transcriptional regulation of genes encoding methylamine methyltransferases (MT) in cells growing on TMA or methanol in the presence of ammonium, overview; gene mttB2C2, operon encoding TMA methylamine methyltransferase, quantitative RT-PCR expression analysis, comparison with other methylamine methyltransferases, overview. Transcriptional regulation of genes encoding methylamine methyltransferases (MT) in cells growing on TMA or methanol in the presence of ammonium, overview
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is upregulated during growth on glycine betaine
transcription of the mtmB1C1 operon is not affected by the nitrogen source but appears to be increased when trimethylamine is the sole carbon and energy source. Transcription of the homologous mtmB1C1 operon occurs at a constant level independently of the nitrogen source; under nitrogen limitation, a 543fold up-regulation of the mtmB2C2 operon, encoding MMA methyltransferase 2, is obtained when methanol is used as carbon source
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information