Information on EC 2.1.1.225 - tRNA:m4X modification enzyme

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

EC NUMBER
COMMENTARY hide
2.1.1.225
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RECOMMENDED NAME
GeneOntology No.
tRNA:m4X modification enzyme
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + adenosine4 in tRNAHis = S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
show the reaction diagram
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC) = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
show the reaction diagram
S-adenosyl-L-methionine + cytidine4 in tRNAPro = S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
tRNA methylation (yeast)
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNAPro/His/Gly(GCC) (cytidine/adenosine4-2'-O)-methyltransferase
The enzyme from Saccharomyces cerevisiae 2'-O-methylates cytidine4 in tRNAPro and tRNAGly(GCC), and adenosine4 in tRNAHis.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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trm13-DELTA strains do not have an obvious growth defect
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenosine4 in tRNAHis
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
show the reaction diagram
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
show the reaction diagram
S-adenosyl-L-methionine + cytidine4 in tRNAPro
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenosine4 in tRNAHis
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
show the reaction diagram
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the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
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-
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
show the reaction diagram
-
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
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-
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
show the reaction diagram
-
the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00001
adenosine4 in tRNAHis
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pH 8.0, 30°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
adenosine4 in tRNAHis
Saccharomyces cerevisiae
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pH 8.0, 30°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
400
adenosine4 in tRNAHis
Saccharomyces cerevisiae
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pH 8.0, 30°C
28112
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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