Information on EC 2.1.1.21 - methylamine-glutamate N-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.21
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RECOMMENDED NAME
GeneOntology No.
methylamine-glutamate N-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
methylamine + L-glutamate = NH3 + N-methyl-L-glutamate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alkyl group transfer
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methyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
methylamine degradation II
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methane metabolism
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Methane metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
methylamine:L-glutamate N-methyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9045-32-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
butylamine + L-glutamate
NH3 + N-butyl-L-glutamate
show the reaction diagram
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at 5% the rate of the reaction with methylamine
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?
ethanolamine + L-glutamate
?
show the reaction diagram
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at 18% the rate of the reaction with methylamine
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-
?
ethylamine + L-glutamate
NH3 + N-ethyl-L-glutamate
show the reaction diagram
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at 16% the rate of reaction with methylamine
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?
methylamine + L-glutamate
NH3 + N-methyl-L-glutamate
show the reaction diagram
methylamine + L-glutamate-gamma-methylester
NH3 + N-methyl-L-glutamate-gamma-methyl ester
show the reaction diagram
methylamine + L-glutamine
?
show the reaction diagram
N-methyl-L-glutamate + ammonia
?
show the reaction diagram
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at 54% the rate of the reaction with cysteamine
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?
N-methyl-L-glutamate + butylamine
?
show the reaction diagram
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at 7% the rate of the reaction with ammonia
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?
N-methyl-L-glutamate + ethanolamine
?
show the reaction diagram
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at 27% the rate of the reaction with ammonia
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?
N-methyl-L-glutamate + ethylamine
?
show the reaction diagram
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at 32% the rate of the reaction with ammonia
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?
N-methyl-L-glutamate + methylamine
?
show the reaction diagram
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at 70% the rate of the reaction with ammonia
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?
N-methyl-L-glutamate + propylamine
?
show the reaction diagram
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at 18% the rate of the reaction with ammonia
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?
propylamine + L-glutamate
NH3 + N-propyl-L-glutamate
show the reaction diagram
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at 8% the rate of the reaction with methylamine
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
methylamine + L-glutamate
NH3 + N-methyl-L-glutamate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-deazaFMN
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1% of activity compared to FMN reconstituted enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ammonia
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competitive with respect to methylamine
arsenite
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hydroxylamine
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reversible
iodoacetate
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N-ethylmaleimide
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N-methylglutamate
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weak product inhibition
p-chloromercuribenzoate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00283 - 0.347
L-glutamate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
49
ammonia
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0113
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mutant strain NMGP5, protein extract from cells grown on methanol and induced with methylamine
0.0118
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mutant strain NMGP4, protein extract from cells grown on methanol and induced with methylamine
0.0223
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wild-type strain, protein extract from cells grown on methanol
0.0557
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mutant strain NMGP3, protein extract from cells grown on methanol and induced with methylamine
0.0705
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wild-type strain, protein extract from cells grown on methanol and induced with methylamine
0.1113
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wild-type strain, protein extract from cells grown on methylamine
0.138
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purified enzyme
3.8
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5-deazaFMN reconstituted enzyme
79.4
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FMN reconstituted enzyme
115
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native enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000 - 35000
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SDS-PAGE
350000 - 390000
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density gradient centrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
multimer
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SDS-PAGE, density gradient centrifugation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cell extracts are prepared
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