HOME
login
history
all enzymes
BRENDA home
History
Enzyme Nomenclature
Information on EC 2.1.1.189 - 23S rRNA (uracil747-C5)-methyltransferase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Bacteria, Archaea
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
EC NUMBER 
COMMENTARY 
2.1.1.189
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
RECOMMENDED NAME
GeneOntology No.
23S rRNA (uracil747-C5)-methyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + uracil747 in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (uracil747-C5)-methyltransferase
The enzyme specifically methylates uracil747 at C5 in 23S rRNA.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
metabolism
evolution
YefA is a COG2265 member. During evolution, COG2265 enzymes have undergone a series of changes in target specificity and YefA is closer to an archetypical m5U methyltransferase. A functional shift has occurred during the evolution of the Bacillus subtilis YefA and YfjO methyltransferases.To reflect its dual specificity, YefA is renamed RlmCD
evolution
-
PAB0760 originates as RlmD-type m5U methyltransferase and undergoes changes in target specificity after its acquisition by a Thermococcales ancestor from a bacterial source. PAB0760 possesses bacterial RlmC-like activity and specifically methylates the nucleotide equivalent to U747 in Pyrococcus abyssi 23S rRNA, but with a sequence most closely related to the bacterial RlmD, the archetypical enzyme that is specific for m5U1939 in 23S rRNA
evolution
-
YefA is a COG2265 member. During evolution, COG2265 enzymes have undergone a series of changes in target specificity and YefA is closer to an archetypical m5U methyltransferase. A functional shift has occurred during the evolution of the Bacillus subtilis YefA and YfjO methyltransferases.To reflect its dual specificity, YefA is renamed RlmCD
-
malfunction
comparison of the methylation patterns in 23S rRNAs from YbjF+ and YbjF- strains shows that the latter differs only in the lack of the 5-methyluracil747 modification
malfunction
-
enzyme inactivation reduces N1-methylated level of G748 by RlmAII in vivo, leading to telithromycin resistance when the nucleotide A2058, located in domain V of 23S rRNA, is dimethylated by the dimethyltransferase Erm(B)
malfunction
-
enzyme inactivation reduces N1-methylated level of G748 by RlmAII in vivo, leading to telithromycin resistance when the nucleotide A2058, located in domain V of 23S rRNA, is dimethylated by the dimethyltransferase Erm(B)
-
metabolism
-
methylation of uridine747 by the enzyme enhances adjacent guanine748 methylation by RlmAII leading to telithromycin-susceptibility
metabolism
-
methylation of uridine747 by the enzyme enhances adjacent guanine748 methylation by RlmAII leading to telithromycin-susceptibility
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
it is not possible to generate a recombinant version of YbjF that retains in vitro activity, so the function of this enzyme is defined in vivo by engineering a ybjF knockout strain
-
-
?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
RlmC recognizes a specific uridine within the loop of 23S rRNA hairpin 35
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
PAB0760 is a m5U methyltransferase and recognizes a specific uridine within the loop of 23S rRNA hairpin 35 in a highly specific manner
product identification by reverse transcriptase primer extension and MALDI mass spectrometry
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
?
additional information
?
-
-
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
-
-
-
additional information
?
-
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
-
-
-
additional information
?
-
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
-
-
-
additional information
?
-
-
the enzyme also methylates uracil1939 in 23S rRNA
-
-
-
additional information
?
-
-
the enzyme also methylates uracil1939 in 23S rRNA
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
O31503
-
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
O31503
-
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
-
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
-
-
-
?
additional information
?
-
-
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
-
-
-
additional information
?
-
O31503
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
-
-
-
additional information
?
-
O31503
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
-
-
-
additional information
?
-
-
the enzyme also methylates uracil1939 in 23S rRNA
-
-
-
additional information
?
-
-
the enzyme also methylates uracil1939 in 23S rRNA
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene yefA, phylogenetic analysis, functional complementation of Bacillus subtilis knockout strain BG12826 yefA::pMutin2 and DELTAyefA::Cm deletion mutant, and of Escherichia coli enzyme-deficient BW 25113 DELTAtrmA/DELTArlmC/DELTArlmD strain
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.189)
html completed
Use of this online version of BRENDA is free for academic research only. Commercial use or download access requires a license. See terms of use.
Information
