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Enzyme Nomenclature
Information on EC 2.1.1.189 - 23S rRNA (uracil747-C5)-methyltransferase
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The expected taxonomic range for this enzyme is: Bacteria, Archaea
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EC NUMBER 
COMMENTARY 
2.1.1.189
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RECOMMENDED NAME
GeneOntology No.
23S rRNA (uracil747-C5)-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + uracil747 in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (uracil747-C5)-methyltransferase
The enzyme specifically methylates uracil747 at C5 in 23S rRNA.
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
malfunction
metabolism
evolution
YefA is a COG2265 member. During evolution, COG2265 enzymes have undergone a series of changes in target specificity and YefA is closer to an archetypical m5U methyltransferase. A functional shift has occurred during the evolution of the Bacillus subtilis YefA and YfjO methyltransferases.To reflect its dual specificity, YefA is renamed RlmCD
evolution
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PAB0760 originates as RlmD-type m5U methyltransferase and undergoes changes in target specificity after its acquisition by a Thermococcales ancestor from a bacterial source. PAB0760 possesses bacterial RlmC-like activity and specifically methylates the nucleotide equivalent to U747 in Pyrococcus abyssi 23S rRNA, but with a sequence most closely related to the bacterial RlmD, the archetypical enzyme that is specific for m5U1939 in 23S rRNA
evolution
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YefA is a COG2265 member. During evolution, COG2265 enzymes have undergone a series of changes in target specificity and YefA is closer to an archetypical m5U methyltransferase. A functional shift has occurred during the evolution of the Bacillus subtilis YefA and YfjO methyltransferases.To reflect its dual specificity, YefA is renamed RlmCD
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malfunction
comparison of the methylation patterns in 23S rRNAs from YbjF+ and YbjF- strains shows that the latter differs only in the lack of the 5-methyluracil747 modification
malfunction
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enzyme inactivation reduces N1-methylated level of G748 by RlmAII in vivo, leading to telithromycin resistance when the nucleotide A2058, located in domain V of 23S rRNA, is dimethylated by the dimethyltransferase Erm(B)
malfunction
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enzyme inactivation reduces N1-methylated level of G748 by RlmAII in vivo, leading to telithromycin resistance when the nucleotide A2058, located in domain V of 23S rRNA, is dimethylated by the dimethyltransferase Erm(B)
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metabolism
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methylation of uridine747 by the enzyme enhances adjacent guanine748 methylation by RlmAII leading to telithromycin-susceptibility
metabolism
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methylation of uridine747 by the enzyme enhances adjacent guanine748 methylation by RlmAII leading to telithromycin-susceptibility
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
it is not possible to generate a recombinant version of YbjF that retains in vitro activity, so the function of this enzyme is defined in vivo by engineering a ybjF knockout strain
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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RlmC recognizes a specific uridine within the loop of 23S rRNA hairpin 35
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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PAB0760 is a m5U methyltransferase and recognizes a specific uridine within the loop of 23S rRNA hairpin 35 in a highly specific manner
product identification by reverse transcriptase primer extension and MALDI mass spectrometry
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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additional information
?
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methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
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additional information
?
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methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
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additional information
?
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methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
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additional information
?
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the enzyme also methylates uracil1939 in 23S rRNA
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additional information
?
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the enzyme also methylates uracil1939 in 23S rRNA
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
O31503
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
O31503
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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?
additional information
?
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methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
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additional information
?
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O31503
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
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additional information
?
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O31503
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
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additional information
?
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the enzyme also methylates uracil1939 in 23S rRNA
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additional information
?
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the enzyme also methylates uracil1939 in 23S rRNA
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene yefA, phylogenetic analysis, functional complementation of Bacillus subtilis knockout strain BG12826 yefA::pMutin2 and DELTAyefA::Cm deletion mutant, and of Escherichia coli enzyme-deficient BW 25113 DELTAtrmA/DELTArlmC/DELTArlmD strain
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