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S-adenosyl-L-methionine + 5'-740GGCACGUUGAAAAGUGCC757-3'
S-adenosyl-L-homocysteine + 5'-740GGCACGUm5UGAAAAGUGCC757-3'
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
additional information
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S-adenosyl-L-methionine + 5'-740GGCACGUUGAAAAGUGCC757-3'
S-adenosyl-L-homocysteine + 5'-740GGCACGUm5UGAAAAGUGCC757-3'
18-mer RNA analogue of the 23S rRNA helix 35
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S-adenosyl-L-methionine + 5'-740GGCACGUUGAAAAGUGCC757-3'
S-adenosyl-L-homocysteine + 5'-740GGCACGUm5UGAAAAGUGCC757-3'
18-mer RNA analogue of the 23S rRNA helix 35
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
it is not possible to generate a recombinant version of YbjF that retains in vitro activity, so the function of this enzyme is defined in vivo by engineering a ybjF knockout strain
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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RlmC recognizes a specific uridine within the loop of 23S rRNA hairpin 35
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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PAB0760 is a m5U methyltransferase and recognizes a specific uridine within the loop of 23S rRNA hairpin 35 in a highly specific manner
product identification by reverse transcriptase primer extension and MALDI mass spectrometry
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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additional information
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methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
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additional information
?
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methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
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additional information
?
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methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
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additional information
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substrate specificity, overview
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additional information
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the enzyme also methylates uracil1939 in 23S rRNA
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additional information
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enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190
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additional information
?
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enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190
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additional information
?
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enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190
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additional information
?
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enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190
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additional information
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replacement of U747 in substrate 5'-740GGCACGUUGAAAAGUGCC757-3' by A, C, or G completely abolishes methylation
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additional information
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replacement of U747 in substrate 5'-740GGCACGUUGAAAAGUGCC757-3' by A, C, or G completely abolishes methylation
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additional information
?
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replacement of U747 in substrate 5'-740GGCACGUUGAAAAGUGCC757-3' by A, C, or G completely abolishes methylation
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additional information
?
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enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190
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additional information
?
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enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and 2.1.1.190
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additional information
?
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the enzyme also methylates uracil1939 in 23S rRNA
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?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
additional information
?
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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S-adenosyl-L-methionine + uracil747 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
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additional information
?
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methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
-
-
?
additional information
?
-
-
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
-
-
?
additional information
?
-
methylation of U747 and U1939 in Bacillus subtilis rRNA is catalysed by a single enzyme, YefA, MALDI mass spectrometry product identification. YefA is not involved in tRNA methylation, or in 23S rRNA m554 methylation
-
-
?
additional information
?
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the enzyme also methylates uracil1939 in 23S rRNA
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-
?
additional information
?
-
enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190
-
-
-
additional information
?
-
-
enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190
-
-
-
additional information
?
-
enzyme introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.18189 and 2.11.190
-
-
-
additional information
?
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-
the enzyme also methylates uracil1939 in 23S rRNA
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?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
evolution
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PAB0760 originates as RlmD-type m5U methyltransferase and undergoes changes in target specificity after its acquisition by a Thermococcales ancestor from a bacterial source. PAB0760 possesses bacterial RlmC-like activity and specifically methylates the nucleotide equivalent to U747 in Pyrococcus abyssi 23S rRNA, but with a sequence most closely related to the bacterial RlmD, the archetypical enzyme that is specific for m5U1939 in 23S rRNA
evolution
YefA is a COG2265 member. During evolution, COG2265 enzymes have undergone a series of changes in target specificity and YefA is closer to an archetypical m5U methyltransferase. A functional shift has occurred during the evolution of the Bacillus subtilis YefA and YfjO methyltransferases.To reflect its dual specificity, YefA is renamed RlmCD
evolution
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YefA is a COG2265 member. During evolution, COG2265 enzymes have undergone a series of changes in target specificity and YefA is closer to an archetypical m5U methyltransferase. A functional shift has occurred during the evolution of the Bacillus subtilis YefA and YfjO methyltransferases.To reflect its dual specificity, YefA is renamed RlmCD
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malfunction
comparison of the methylation patterns in 23S rRNAs from YbjF+ and YbjF- strains shows that the latter differs only in the lack of the 5-methyluracil747 modification
malfunction
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enzyme inactivation reduces N1-methylated level of G748 by RlmAII in vivo, leading to telithromycin resistance when the nucleotide A2058, located in domain V of 23S rRNA, is dimethylated by the dimethyltransferase Erm(B)
malfunction
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enzyme inactivation reduces N1-methylated level of G748 by RlmAII in vivo, leading to telithromycin resistance when the nucleotide A2058, located in domain V of 23S rRNA, is dimethylated by the dimethyltransferase Erm(B)
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metabolism
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methylation of uridine747 by the enzyme enhances adjacent guanine748 methylation by RlmAII leading to telithromycin-susceptibility
metabolism
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methylation of uridine747 by the enzyme enhances adjacent guanine748 methylation by RlmAII leading to telithromycin-susceptibility
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D381A
2% residual activity towards U747 RNA substrate, 40% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190
F145A
5% residual activity towards U747 RNA substrate, 120% activity towards U1939 RNA substrate, reaction of EC 2.11.190
H151A
5% residual activity towards U747 RNA substrate, 55% activity towards U1939 RNA substrate, reaction of EC 2.11.190
N249A
35% residual activity towards U747 RNA substrate, 55% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190
Q162A
18% residual activity towards U747 RNA substrate, 160% activity towards U1939 RNA substrate, reaction of EC 2.11.190
Q283A
8% residual activity towards U747 RNA substrate, 50% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190
R127A
8% residual activity towards U747 RNA substrate, 2% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190
D381A
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2% residual activity towards U747 RNA substrate, 40% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190
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F145A
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5% residual activity towards U747 RNA substrate, 120% activity towards U1939 RNA substrate, reaction of EC 2.11.190
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H151A
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5% residual activity towards U747 RNA substrate, 55% activity towards U1939 RNA substrate, reaction of EC 2.11.190
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Q162A
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18% residual activity towards U747 RNA substrate, 160% activity towards U1939 RNA substrate, reaction of EC 2.11.190
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R127A
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8% residual activity towards U747 RNA substrate, 2% residual activity towards U1939 RNA substrate, reaction of EC 2.11.190
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additional information
both full-length RlmCD and its shorter construct RlmCDs (residue 1-454) exhibit strong U74 methyltransferase activity when compared with the glutamine mutant of E443
additional information
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both full-length RlmCD and its shorter construct RlmCDs (residue 1-454) exhibit strong U74 methyltransferase activity when compared with the glutamine mutant of E443
additional information
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both full-length RlmCD and its shorter construct RlmCDs (residue 1-454) exhibit strong U74 methyltransferase activity when compared with the glutamine mutant of E443
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Madsen, C.T.; Mengel-Jorgensen, J.; Kirpekar, F.; Douthwaite, S.
Identifying the methyltransferases for m(5)U747 and m(5)U1939 in 23S rRNA using MALDI mass spectrometry
Nucleic Acids Res.
31
4738-4746
2003
Escherichia coli (P75817)
brenda
Desmolaize, B.; Fabret, C.; Bregeon, D.; Rose, S.; Grosjean, H.; Douthwaite, S.
A single methyltransferase YefA (RlmCD) catalyses both m5U747 and m5U1939 modifications in Bacillus subtilis 23S rRNA
Nucleic Acids Res.
39
9368-9375
2011
Bacillus subtilis (O31503), Bacillus subtilis, Bacillus subtilis 168 (O31503)
brenda
Auxilien, S.; Rasmussen, A.; Rose, S.; Brochier-Armanet, C.; Husson, C.; Fourmy, D.; Grosjean, H.; Douthwaite, S.
Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases
RNA
17
45-53
2011
Escherichia coli, Pyrococcus abyssi
brenda
Shoji, T.; Takaya, A.; Sato, Y.; Kimura, S.; Suzuki, T.; Yamamoto, T.
RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAII in 23S rRNA in Streptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility
Nucleic Acids Res.
43
8964-8972
2015
Streptococcus pneumoniae, Streptococcus pneumoniae S1
brenda
Jiang, Y.; Li, F.; Wu, J.; Shi, Y.; Gong, Q.
Structural insights into substrate selectivity of ribosomal RNA methyltransferase RlmCD
PLoS ONE
12
e0185226
2017
Streptococcus pneumoniae (Q97R12), Streptococcus pneumoniae, Streptococcus pneumoniae ATCC BAA-334 (Q97R12)
brenda
Jiang, Y.; Yu, H.; Li, F.; Cheng, L.; Zhu, L.; Shi, Y.; Gong, Q.
Unveiling the structural features that determine the dual methyltransferase activities of Streptococcus pneumoniae RlmCD
PLoS Pathog.
14
e1007379
2018
Streptococcus pneumoniae (Q97R12), Streptococcus pneumoniae, Streptococcus pneumoniae ATCC BAA-334 (Q97R12)
brenda