Information on EC 2.1.1.181 - 23S rRNA (adenine1618-N6)-methyltransferase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.1.1.181
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RECOMMENDED NAME
GeneOntology No.
23S rRNA (adenine1618-N6)-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + adenine1618 in 23S rRNA = S-adenosyl-L-homocysteine + N6-methyladenine1618 in 23S rRNA
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (adenine1618-N6)-methyltransferase
The recombinant YbiN protein is able to methylate partially deproteinized 50 S ribosomal subunit, but neither the completely assembled 50 S subunits nor completely deproteinized 23 S rRNA [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
knockout of the ybiN gene leads to loss of modification at adenine1618. The modification is restored if ybiN knock-out strain has been co-transformed with a plasmid expressing the ybiN gene. Both lack of the ybiN gene and it's overexpression leads to growth retardation and loss of cell fitness comparative to the parental strain
physiological function
A1618 modification could be necessary for the exit tunnel interaction with some unknown regulatory peptides
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine1618 in 23S rRNA
S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine1618 in 23S rRNA
S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
show the reaction diagram
P75782
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?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 34000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant YbiN carrying a N-terminal His6 tag is expressed in Escherichia coli and purified
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant YbiN carrying a N-terminal His6 tag is expressed in Escherichia coli and purified
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