Information on EC 2.1.1.163 - demethylmenaquinone methyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.1.1.163
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RECOMMENDED NAME
GeneOntology No.
demethylmenaquinone methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a demethylmenaquinol + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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menaquinol-10 biosynthesis
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menaquinol-11 biosynthesis
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menaquinol-12 biosynthesis
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menaquinol-13 biosynthesis
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menaquinol-6 biosynthesis
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menaquinol-7 biosynthesis
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menaquinol-8 biosynthesis
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menaquinol-9 biosynthesis
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Metabolic pathways
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Ubiquinone and other terpenoid-quinone biosynthesis
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vitamin K metabolism
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methione:demethylmenaquinone methyltransferase
The enzyme catalyses the last step in menaquinone biosynthesis. It is able to accept substrates with varying polyprenyl side chain length (the chain length is determined by polyprenyl diphosphate synthase)[1]. The enzyme from Escherichia coli also catalyses the conversion of 2-methoxy-6-octaprenyl-1,4-benzoquinone to 5-methoxy-2-methyl-3-octaprenyl-1,4-benzoquinone during the biosynthesis of ubiquinone [4]. The enzyme probably acts on menaquinol rather than menaquinone.
CAS REGISTRY NUMBER
COMMENTARY hide
37259-80-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene ubiE
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Manually annotated by BRENDA team
; gene heps-2
UniProt
Manually annotated by BRENDA team
gene dmtH, isolated from a transposon-inserted mutant library
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Manually annotated by BRENDA team
gene dmtH, isolated from a transposon-inserted mutant library
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Manually annotated by BRENDA team
no activity in Rhizobium sp.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
ORF-1, ORF-2, and ORF-3 genes, designated heps-1, menG, and heps-2, respectively, form another cluster involved in menaquinone biosynthesis in addition to the cluster of menB, menC, menD, and menE already identified in the Bacillus subtilis and Escherichia coli chromosomes
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-heptaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine
2-heptaprenyl-6-methoxy-3-methyl-1,4-benzoquinone + S-adenosyl-L-homocysteine
show the reaction diagram
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?
2-octaprenyl-1,4-naphthoquinone + S-adenosyl-L-methionine
2-octaprenyl-3-methyl-1,4-naphthoquinone + S-adenosyl-L-homocysteine
show the reaction diagram
2-octaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine
2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-homocysteine
show the reaction diagram
6-methoxy-2-octaprenyl-1,4-benzoquinol + S-adenosyl-L-methionine
6-methoxy-3-methyl-2-octaprenyl-1,4-benzoquinol + S-adenosyl-L-homocysteine
show the reaction diagram
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?
demethylmenaquinol + S-adenosyl-L-methionine
menaquinol + S-adenosyl-L-homocysteine
show the reaction diagram
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-octaprenyl-1,4-naphthoquinone + S-adenosyl-L-methionine
2-octaprenyl-3-methyl-1,4-naphthoquinone + S-adenosyl-L-homocysteine
show the reaction diagram
2-octaprenyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine
2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone + S-adenosyl-L-homocysteine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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the transcript level increases after 3 h of low-temperature exposure, reaching a maximum at 12 h
Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli; gene heps-2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain JM109
gene dmtH, cloned from a transposon-inserted mutant library, DNA and mino acid sequence determination and analysis. Real-time quantitative PCR shows that dmtH is a bacteroid-specific gene with highest expression at 25 days after inoculation of strain 7653R
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene expression is inducible by low temperatures, but not by high salt or dought. A T-DNA-tagged DMKT1 gene co-seggregates with the cold-inducible gus gene
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G142D
mutant accumulates 2-octaprenyl-6-methoxy-1,4-benzoquinone and demethylmenaquinone as predominant intermediates
additional information
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