Information on EC 2.1.1.161 - dimethylglycine N-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.161
-
RECOMMENDED NAME
GeneOntology No.
dimethylglycine N-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycine betaine biosynthesis IV (from glycine)
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glycine betaine biosynthesis V (from glycine)
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Glycine, serine and threonine metabolism
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:N,N-dimethylglycine N-methyltransferase (betaine-forming)
This enzyme, from the marine cyanobacterium Synechococcus sp. WH8102, differs from EC 2.1.1.157, sarcosine/dimethylglycine N-methyltransferase in that it cannot use sarcosine as an alternative substrate [1]. Betaine is a 'compatible solute' that enables cyanobacteria to cope with osmotic stress by maintaining a positive cellular turgor.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
show the reaction diagram
additional information
?
-
several other amino acids tested as substrates and non of them serves as methyl group acceptor
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alanine
-
slight inhibition
cysteine
-
slight inhibition
isovaleric acid
-
slight inhibition
methionine
-
slight inhibition
n-butyric acid
-
-
N-methylglycine
-
slight inhibition
phenylalanine
-
slight inhibition
Propionic acid
-
slight inhibition
S-adenosyl-L-homocysteine
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t-butyrylacetic acid
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-
additional information
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no inhibition with betaine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5 - 2.11
N,N-dimethylglycine
0.18 - 0.41
S-adenosyl-L-methionine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
with N,N-dimethylglycin as substrate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 11
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very low activity below pH 7
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
-
gel filtration
31210
-
calculated from the deduced amino acid sequence
34000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein using His-tag
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS
expressed in Escherichia coli BL21(DE3)
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
M172R
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almost inactive
P171Q
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strongly increased Km for dimethylglycine, slight increase in the Km for S-adenosyl-L-methionine
P171Q/M172R
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almost inactive