Information on EC 2.1.1.154 - isoliquiritigenin 2'-O-methyltransferase

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The expected taxonomic range for this enzyme is: Medicago sativa

EC NUMBER
COMMENTARY hide
2.1.1.154
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RECOMMENDED NAME
GeneOntology No.
isoliquiritigenin 2'-O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + isoliquiritigenin = S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
isoflavonoid biosynthesis I
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-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:isoliquiritigenin 2'-O-methyltransferase
Not identical to EC 2.1.1.65, licodione 2'-O-methyltransferase [2]. While EC 2.1.1.154, isoliquiritigenin 2'-O-methyltransferase can use licodione as a substrate, EC 2.1.1.65 cannot use isoliquiritigenin as a substrate.
CAS REGISTRY NUMBER
COMMENTARY hide
139317-14-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Glycyrrhiza echinata
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 2',4'-dihydroxy-4-methoxychalcone
S-adenosyl-L-homocysteine + 4'-hydroxy-2',4-dimethoxychalcone
show the reaction diagram
-
77% of the activity with isoliquiritigenin
-
-
?
S-adenosyl-L-methionine + 4',7-dihydroxyflavanone
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
32% of the activity with isoliquiritigenin
-
-
?
S-adenosyl-L-methionine + isoliquiritigenin
S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + isoliquiritigenin
S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin
show the reaction diagram
-
the product of the reaction, 2'-O-methylisoliquiritigenin, is the most potent inducer of nodulation genes of Rhizobium meliloti, the symbiont of Medicago sativa which forms nitrogen-fixing nodules
-
-
?
additional information
?
-
-
the enzyme specifically involved in the biosynthesis of an inducer of Rhizobium meliloti nodulation genes
-
-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4,4'-dihydroxy-2'-methoxychalcone
-
noncompetitive
Co2+
-
1 mM, 22% inhibition
Cu2+
-
1 mM, 12% inhibition
Fe2+
-
1 mM, 44% inhibition
S-adenosyl-L-homocysteine
-
competitive with respect to S-adenosyl-L-methionine
Zn2+
-
1 mM, 45% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2
isoliquiritigenin
-
-
17.7
S-adenosyl-L-methionine
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-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0652
4,4'-dihydroxy-2'-methoxychalcone
-
-
0.0022
S-adenosyl-L-homocysteine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
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chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
regardless of plant age, expression to low extent
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
98% of the activity is in the soluble fraction
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 43000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
half-life: 3-4 days
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stabilized in presence of 5 mM EDTA
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 12% loss of activity after 1.5 months
-
4°C, half-life: 3-4 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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