Information on EC 2.1.1.147 - corydaline synthase

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The expected taxonomic range for this enzyme is: Corydalis cava

EC NUMBER
COMMENTARY hide
2.1.1.147
-
RECOMMENDED NAME
GeneOntology No.
corydaline synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + palmatine + 2 NADPH + H+ = S-adenosyl-L-homocysteine + corydaline + 2 NADP+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Isoquinoline alkaloid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:protoberberine 13-C-methyltransferase
Also acts on 7,8-dihydropalmatine.
CAS REGISTRY NUMBER
COMMENTARY hide
155807-67-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + berberine + NADPH + H+
S-adenosyl-L-homocysteine + corydaline + NADP+
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + columbamine + NADPH + H+
S-adenosyl-L-homocysteine + isocorypalmine + NADP+
show the reaction diagram
-
27% of the activity with berberine
-
-
?
S-adenosyl-L-methionine + coptisine + NADPH + H+
S-adenosyl-L-homocysteine + tetrahydrocorysamine + NADP+
show the reaction diagram
-
21% of the activity with berberine
-
-
?
S-adenosyl-L-methionine + dihydroberberine + NADPH + H+
S-adenosyl-L-homocysteine + thalictricavine + NADP+
show the reaction diagram
-
93% of the activity with berberine
-
?
S-adenosyl-L-methionine + jatrorrhizine + NADPH + H+
S-adenosyl-L-homocysteine + 7,8-dehydrothalictricavine + NADP+
show the reaction diagram
-
67% of the activity with berberine
-
-
?
S-adenosyl-L-methionine + palmatine + NADPH + H+
S-adenosyl-L-homocysteine + corydaline + NADP+
show the reaction diagram
-
93% of the activity with berberine
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-homocysteine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005
Berberine
-
-
0.016
NADPH
-
-
0.0027
S-adenosyl-L-methionine
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0026
S-adenosyl-L-homocysteine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9.5
-
pH 7.0: about 45% of maximal activity, pH 9.5: about 30% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 50
-
20°C: about 35% of maximal activity, 50°C: about 75% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
-
gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE