Information on EC 2.1.1.143 - 24-methylenesterol C-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.143
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RECOMMENDED NAME
GeneOntology No.
24-methylenesterol C-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 24-methylenelophenol = S-adenosyl-L-homocysteine + (Z)-24-ethylidenelophenol
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of methyl group
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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plant sterol biosynthesis
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Steroid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:24-methylenelophenol C-methyltransferase
This is the second methylation step of plant sterol biosynthesis (cf EC 2.1.1.142, cycloartenol 24-C-methyltransferase).
CAS REGISTRY NUMBER
COMMENTARY hide
37257-07-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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SMT2 and SMT3 genes are highly homologous and encode highly similar sterol 24-carbon methyltransferases
malfunction
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deficiency of SMT2 in the cvp1 mutant results in moderate developmental defects, including aberrant cotyledon vein patterning, serrated floral organs, and reduced stature, but plants are viable, suggesting that SMT3 activity can substitute for the loss of SMT2, overview. Mutation in both SMT2 and SMT3 causes extreme alteration in sterol composition, sterol profiles of smt mutants, overview. Mutants deficient in both SMT1 and SMT2 are embryo lethal. Phenotypes, detailed overview
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 24(28)-methylene lophenol
S-adenosyl-L-homocysteine + 24(28)-ethylidene lophenol
show the reaction diagram
best substrate
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-
?
S-adenosyl-L-methionine + 24-methylene-cholesta-5,7-dien-3beta-ol
S-adenosyl-L-homocysteine + cholesta-8,24-dien-3beta-ol
show the reaction diagram
S-adenosyl-L-methionine + 24-methylenelophenol
S-adenosyl-L-homocysteine + (Z)-24-ethylidenelophenol
show the reaction diagram
S-adenosyl-L-methionine + 24-methylenelophenol
S-adenosyl-L-homocysteine + 24-ethylidenelophenol
show the reaction diagram
S-adenosyl-L-methionine + 4alpha-methyl zymosterol
S-adenosyl-L-homocysteine + ?
show the reaction diagram
60% of the effectiveness of 24(28)-methylene lophenol
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-
?
S-adenosyl-L-methionine + cycloartenol
S-adenosyl-L-homocysteine + 24-methylene cycloartenol
show the reaction diagram
S-adenosyl-L-methionine + ergosta-7,24(28)-dienol
S-adenosyl-L-homocysteine + ?
show the reaction diagram
equally effective as 24(28)-methylene lophenol
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-
?
S-adenosyl-L-methionine + fecosterol
S-adenosyl-L-homocysteine + ?
show the reaction diagram
equally effective as 24(28)-methylene lophenol
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-
?
S-adenosyl-L-methionine + lanosterol
S-adenosyl-L-homocysteine + ?
show the reaction diagram
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12.4% of the activity with 24-methylenelophenol
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?
S-adenosyl-L-methionine + obtusifoliol
S-adenosyl-L-homocysteine + ?
show the reaction diagram
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11.9% of the activity with 24-methylenelophenol
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?
S-adenosyl-L-methionine + zymosterol
S-adenosyl-L-homocysteine + ?
show the reaction diagram
70% of the effectiveness of 24(28)-methylene lophenol
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?
S-adenosyl-L-methionine + zymosterol
S-adenosyl-L-homocysteine + fecosterol + episterol + stigmasta-8,24-dienol + DELTA7-avenasterol
show the reaction diagram
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5.2% of the activity with 24-methylenelophenol
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 24-methylene-cholesta-5,7-dien-3beta-ol
S-adenosyl-L-homocysteine + cholesta-8,24-dien-3beta-ol
show the reaction diagram
S-adenosyl-L-methionine + 24-methylenelophenol
S-adenosyl-L-homocysteine + (Z)-24-ethylidenelophenol
show the reaction diagram
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?
S-adenosyl-L-methionine + 24-methylenelophenol
S-adenosyl-L-homocysteine + 24-ethylidenelophenol
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
24(28)-methylene cycloartanol
;
25-Azacycloartenol
;
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.028 - 0.03
24(28)-methylene lophenol
0.012
24-methylene lophenol
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0.0049 - 0.05
24-methylenelophenol
0.0114 - 0.042
Cycloartenol
0.0124
lanosterol
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0.0119
obtusifoliol
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0.0052
Zymosterol
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013 - 0.022
24(28)-methylene lophenol
0.001 - 0.005
24-methylenelophenol
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.077 - 0.25
24(28)-methylene cycloartanol
0.00001 - 0.00002
25-Azacycloartenol
0.1 - 0.297
sitosterol
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 7.7
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3
calculated
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
4 * 40400, calculated, 4 * 40000, SDS-PAGE
40300
x * 40300, calculated
40400
4 * 40400, calculated, 4 * 40000, SDS-PAGE
160000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 40300, calculated
tetramer
4 * 40400, calculated, 4 * 40000, SDS-PAGE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli; expression in Escherichia coli
expression in Nicotiana tabacum
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expression in yeast null mutant erg6
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genes SMT2-1 and SMT2-2, generation of a beta-conglycinin-AtHMGR1:glycinin-GmSMT1 construct and a glycinin-GmSMT1-pZY101-Asc construct, and of a a three gene casette vector beta-conglycinin-AtHMGR1:glycinin-GmSMT1:beta-phaseolin-GmSMT2-2, overview. cis-Acting elements occur in SMT2 upstream sequences, motifs responsible for basal and regulated expression, overview. Expression of SMT2 in Arabidopsis thaliana ecotype Columbia plants, expression of genes SMT2-1 and SMT2-2 complements the SMT deficient cvp1 mutant Arabidopsis plants. The transgenic plants harboring the SMT2-1 promoter show enzyme-GUS expression predominantly in the cotyledons and the base of the hypocotyls in seedlings, and the tips of young leaves. Transgenic plants with the SMT2-2 promoter had a similar expression pattern, but with strong enzyme-Gus expression throughout the leaves and stem, specifically in the vascular tissue, in both etiolated and light-grown plants; genes SMT2-1 and SMT2-2, generation of a beta-conglycinin-AtHMGR1:glycinin-GmSMT1 construct and a glycinin-GmSMT1-pZY101-Asc construct, and of a a three gene casette vector beta-conglycinin-AtHMGR1:glycinin-GmSMT1:beta-phaseolin-GmSMT2-2, overview. cis-Acting elements occur in SMT2 upstream sequences, motifs responsible for basal and regulated expression, overview. Expression of SMT2 in Arabidopsis thaliana ecotype Columbia plants, expression of genes SMT2-1 and SMT2-2 complements the SMT deficient cvp1 mutant Arabidopsis plants. The transgenic plants harboring the SMT2-1 promoter show enzyme-GUS expression predominantly in the cotyledons and the base of the hypocotyls in seedlings, and the tips of young leaves. Transgenic plants with the SMT2-2 promoter had a similar expression pattern, but with strong enzyme-Gus expression throughout the leaves and stem, specifically in the vascular tissue, in both etiolated and light-grown plants
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
environmental cues, including dehydration, cold, and abscisic acid induce differential changes in transcript levels of the SMT2 during soybean seedling growth; environmental cues, including dehydration, cold, and abscisic acid induce differential changes in transcript levels of the SMT2 during soybean seedling growth
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Y83F
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Km-value for 24-methylenelophenol is identical to wild-type value, turnover number is 5fold higher than wild-type value
Y83L
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inactive mutant enzyme
additional information