Information on EC 2.1.1.139 - 3'-demethylstaurosporine O-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.139
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RECOMMENDED NAME
GeneOntology No.
3'-demethylstaurosporine O-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 3'-demethylstaurosporine = S-adenosyl-L-homocysteine + staurosporine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer of methyl group
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of antibiotics
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staurosporine biosynthesis
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Staurosporine biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:3'-demethylstaurosporine O-methyltransferase
Catalyses the final step in the biosynthesis of staurosporine, an alkaloidal antibiotic that is a potent inhibitor of protein kinases, especially protein kinase C.
CAS REGISTRY NUMBER
COMMENTARY hide
212906-74-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3'-demethylstaurosporine
S-adenosyl-L-homocysteine + staurosporine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3'-demethylstaurosporine
S-adenosyl-L-homocysteine + staurosporine
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1H-indole
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62% activity left at 1.1 mM
3'-demethylstaurosporine
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69% activity at 1.1 mM
anthranilate
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75% activity left at 1.1 mM
Indole-3-pyruvate
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44% activity left at 1.1 mM
S-(5'-adenosyl)-L-homocysteine
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strong inhibition, 8% activity left at 1.1 mM, probably competitive inhibition
sinefungin
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antiprotozoal antibiotic from Streptomyces griseolus, efficient inhibition with concentrations higher than 1 mg/100 ml
tryptophan
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64% activity left at 1.1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
tryptophan
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2fold increase at 0.2 mM
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
anion exchange chromatography, partial purification
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