Information on EC 2.1.1.122 - (S)-tetrahydroprotoberberine N-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.122
-
RECOMMENDED NAME
GeneOntology No.
(S)-tetrahydroprotoberberine N-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine = S-adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
noscapine biosynthesis
-
-
Isoquinoline alkaloid biosynthesis
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:(S)-7,8,13,14-tetrahydroprotoberberine cis-N-methyltransferase
Involved in the biosynthesis of isoquinoline alkaloids in plants.
CAS REGISTRY NUMBER
COMMENTARY hide
106878-42-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
Berberis stenophylla
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Corydalis vaginans
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Nandina domestica
-
-
-
Manually annotated by BRENDA team
no activity in Nicotiana tabacum
-
-
-
Manually annotated by BRENDA team
no activity in Tinospora cordifolia
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (R,S)-canadine
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + (R,S)-stylopine
?
show the reaction diagram
S-adenosyl-L-methionine + (R,S)-tetrahydropalmatine
?
show the reaction diagram
S-Adenosyl-L-methionine + (S)-7,8,13,14-tetrahydroprotoberberine
S-Adenosyl-L-homocysteine + cis-N-methyl-(S)-7,8,13,14-tetrahydroprotoberberine
show the reaction diagram
S-Adenosyl-L-methionine + (S)-stylopine
?
show the reaction diagram
S-Adenosyl-L-methionine + tetrahydrojatrorrhizine
?
show the reaction diagram
-
-
-
-
-
S-Adenosyl-L-methionine + tetrahydropalatrubine
?
show the reaction diagram
-
-
-
-
-
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg2+
-
slight increase in activity with increasing concentrations of Mg2+ in sodium phosphate buffer, inhibitory in Hepes buffer
Mn2+
inhibits activity by 41%, inhibition prevented by inclusion of 10 mM EDTA; inhibits TNMT activity by 41%, can be prevented by the inclusion of EDTA
Ni2+
inhibits activity by 72%, inhibition prevented by inclusion of 10 mM EDTA; inhibits TNMT activity by 72%, can be prevented by the inclusion of EDTA
S-adenosyl-L-homocysteine
Zn2+
-
in sodium phosphate buffer and in Hepes buffer
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0006
(R,S)-stylopine
varying concentration from 0.5 to 32 microM at a saturating concentration of 20 microM of S-adenosyl-L-methionine
0.0021
(S)-7,8,13,14-tetrahydroprotoberberine
-
-
0.0064 - 0.007
(S)-canadine
0.0006 - 0.004
(S)-Stylopine
0.0012 - 0.012
S-adenosyl-L-methionine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
; determined by using 100 mM sodium phosphate (pH 5-7) and 100 mM Tris-HCl (pH 7-9) containing 12 mM beta-mercaptoethanol
8
Corydalis vaginans
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
recombinant protein, half-maximal activity estimated at pH 6.8 and 8.2
6.5 - 8
-
pH 6.5: about 55% of maximal activity, pH 8.0: about 65% of maximal activity
6.6 - 9.1
-
about 50% of maximal activity at pH 6.6 and 9.1
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 50
recombinant protein, half-maximal activity estimated at 12C and 47C
15 - 40
-
15C: about 30% of maximal activity, 40C: about 25% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
elicitor treatment
Manually annotated by BRENDA team
TNMTgene transcripts at lower levels, but lower levels of TNMT activity
Manually annotated by BRENDA team
TNMTgene transcripts to a lesser extent, but highest levels of TNMT activity
Manually annotated by BRENDA team
TNMTgene transcripts most abundantly, but lower levels of TNMT activity
Manually annotated by BRENDA team
neither TNMT transcripts nor enzyme activity in seedlings 1 day after seed inbibition, whereas both TNMT transcript levels and enzyme activity 4 days after inbibition, remaining relatively constant during seedling growth; transcripts and enzyme activity in
Manually annotated by BRENDA team
TNMTgene transcripts at lower levels, but highest levels of TNMT activity
Manually annotated by BRENDA team
transcripts and enzyme activity in
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
the enzyme is not compartmentalized
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
-
gel filtration
72000
Corydalis vaginans
-
gel filtration
78000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 39000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
half-life: 5 days
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 20% glycerol, half-life: 5 days
-
4C, 20% glycerol, half-life: 10 days
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by nickel affinity chromatography; recombinant protein, gel filtration and SDS-PAGE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Escherichia coli strain ER2566 pLysS transformed with pTNMT plasmid; expressed in Escherichia coli ER2566 pLysS cells, pRSETB vector
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
low TNMT activity