Information on EC 2.1.1.102 - demethylmacrocin O-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)
Please login to have access to the AMENDA and FRENDA data


The expected taxonomic range for this enzyme is: Streptomyces fradiae

top print hide
EC NUMBER
COMMENTARY hide
2.1.1.102
-
top print hide
RECOMMENDED NAME
GeneOntology No.
demethylmacrocin O-methyltransferase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + demethylmacrocin = S-adenosyl-L-homocysteine + macrocin
show the reaction diagram
the 2-hydroxyl group of a 6-deoxy-D-allose residue in demethylmacrocin acts as a methyl acceptor. Not identical with EC 2.1.101 macrocin O-methyltransferase
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of 12-, 14- and 16-membered macrolides
-
-
Biosynthesis of antibiotics
-
-
tylosin biosynthesis
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:demethylmacrocin 2'''-O-methyltransferase
Requires Mg2+. The enzyme, isolated from the bacterium Streptomyces fradiae, is involved in the biosynthesis of the macrolide antibiotic tylosin. The 2-hydroxy group of a 6-deoxy-D-allose moiety in demethylmacrocin acts as the methyl acceptor. Also acts on demethyllactenocin, giving lactenocin. Not identical with EC 2.1.1.101 macrocin O-methyltransferase.
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
120313-64-6
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + demethyllactenocin
S-adenosyl-L-homocysteine + lactenocin
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
show the reaction diagram
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
show the reaction diagram
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required for maximal activity
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20-Dihydro-23-deoxymycaminosyltylonolide
-
-
20-dihydrolactenocin
-
inhibition of macrocin formation
20-Dihydromacrocin
-
-
23-demycinosyloxytylosin
-
-
23-Deoxy-O-mycaminosyltylonolide
-
-
demycinosyltylosin
-
-
lactenocin
-
inhibition of macrocin formation
macrocin
-
inhibition of lactenocin formation
mycaminosyltylactone
-
-
mycaminosyltylonolide
-
-
O-Mycaminosyltylonolide
-
-
S-adenosyl-L-homocysteine
-
not D-enantiomer
sinefungin
-
competitive inhibition, poor inhibitor
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
demethyllactenocin
-
-
0.006
Demethylmacrocin
-
-
0.111
S-adenosylmethionine
-
-
top print hide
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.186
23-demycinosyloxytylosin
-
-
0.073
23-Deoxy-O-mycaminosyltylonolide
-
-
0.057 - 0.108
A914C
-
0.022 - 0.024
lactenocin
0.037 - 0.067
macrocin
0.065
mycaminosyltylonolide
-
-
0.11 - 0.226
S-adenosyl-L-homocysteine
0.077
sinefungin
-
-
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
-
3 * 42000, SDS-PAGE
122000 - 126000
-
gel filtration
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
-
3 * 42000, SDS-PAGE
top print hide
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, quite stable in presence of S-adenosylmethionine and 10% ethanol, pH 7.0-8.0, stable for a minimum of 2 months
-
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top print hide
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pharmacology
-
tylosin fermentation, antibiotic biosynthesis
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.102)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)