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Information on EC 2.1.1.102 - demethylmacrocin O-methyltransferase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Streptomyces fradiae
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demethylmacrocin O-methyltransferase
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S-adenosyl-L-methionine + demethylmacrocin = S-adenosyl-L-homocysteine + macrocin
the 2-hydroxyl group of a 6-deoxy-D-allose residue in demethylmacrocin acts as a methyl acceptor. Not identical with EC 2.1.101 macrocin O-methyltransferase
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methyl group transfer
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Biosynthesis of 12-, 14- and 16-membered macrolides
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Biosynthesis of antibiotics
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S-adenosyl-L-methionine:demethylmacrocin 2'''-O-methyltransferase
Requires Mg2+. The enzyme, isolated from the bacterium Streptomyces fradiae, is involved in the biosynthesis of the macrolide antibiotic tylosin. The 2-hydroxy group of a 6-deoxy-D-allose moiety in demethylmacrocin acts as the methyl acceptor. Also acts on demethyllactenocin, giving lactenocin. Not identical with EC 2.1.1.101 macrocin O-methyltransferase.
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demethylmacrocin methyltransferase
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brenda
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S-adenosyl-L-methionine + demethyllactenocin
S-adenosyl-L-homocysteine + lactenocin
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S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
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S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
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enzyme catalyzes 2'''-O-methylation of bound 6-deoxy-D-allose
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S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
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penultimate step in tylosin biosynthesis
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S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
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enzyme catalyzes 2'''-O-methylation of bound 6-deoxy-D-allose
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S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
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penultimate step in tylosin biosynthesis
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Mg2+
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required for maximal activity
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20-Dihydro-23-deoxymycaminosyltylonolide
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20-dihydrolactenocin
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inhibition of macrocin formation
23-demycinosyloxytylosin
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23-Deoxy-O-mycaminosyltylonolide
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lactenocin
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inhibition of macrocin formation
macrocin
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inhibition of lactenocin formation
mycaminosyltylonolide
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O-Mycaminosyltylonolide
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S-adenosyl-L-homocysteine
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not D-enantiomer
sinefungin
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competitive inhibition, poor inhibitor
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0.01
demethyllactenocin
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0.006
Demethylmacrocin
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0.111
S-adenosylmethionine
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0.186
23-demycinosyloxytylosin
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0.073
23-Deoxy-O-mycaminosyltylonolide
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0.065
mycaminosyltylonolide
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0.11 - 0.226
S-adenosyl-L-homocysteine
0.057
A914C
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fixed substrate demethylmacrocin
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0.108
A914C
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fixed substrate S-adenosylmethionine
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0.022
lactenocin
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fixed substrate demethylmacrocin
0.024
lactenocin
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fixed substrate S-adenosylmethionine
0.037
macrocin
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fixed substrate S-adenosylmethionine
0.067
macrocin
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fixed substrate demethyllactenocin
0.11
S-adenosyl-L-homocysteine
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fixed substrate demethylmacrocin
0.226
S-adenosyl-L-homocysteine
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fixed substrate S-adenosylmethionine
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42000
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3 * 42000, SDS-PAGE
122000 - 126000
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gel filtration
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trimer
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3 * 42000, SDS-PAGE
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4°C, quite stable in presence of S-adenosylmethionine and 10% ethanol, pH 7.0-8.0, stable for a minimum of 2 months
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pharmacology
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tylosin fermentation, antibiotic biosynthesis
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TYLE_STRFR
395
43233
Swiss-Prot
A0A149VYT9_9PROT
351
40522
TrEMBL
A0A0P1HBD3_9RHOB
237
26953
TrEMBL
A0A177HHL2_9ACTN
282
31578
TrEMBL
A0A1D8AVL6_9BACT
321
35226
TrEMBL
A0A1V5ZQ58_9BACT
212
24600
TrEMBL
A0A0P1G2D7_9RHOB
237
26557
TrEMBL
A0A1D8BZ59_9PSEU
223
25045
TrEMBL
A0A1Y5RZ21_9RHOB
544
61826
TrEMBL
A0A0P0M9L0_9BURK
475
54067
TrEMBL
A0A143PY46_9BACT
269
31771
TrEMBL
A0A1X6Z2B1_9RHOB
258
29206
TrEMBL
A0A120MY83_9SPHI
239
28262
TrEMBL
A0A1J5T8D0_9ZZZZ
262
29768
TrEMBL
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Kreuzman, A.J.; Turner, J.R.; Yeh, W.K.
Two distinctive O-methyltransferases catalyzing penultimate and terminal reactions of macrolide antibiotic (tylosin) biosynthesis. Substrate specificity, enzyme inhibition, and kinetic mechanism
J. Biol. Chem.
263
15626-15633
1988
Streptomyces fradiae
brenda
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