Information on EC 1.9.98.1 - iron-cytochrome-c reductase

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The expected taxonomic range for this enzyme is: Acidithiobacillus ferrooxidans

EC NUMBER
COMMENTARY hide
1.9.98.1
-
RECOMMENDED NAME
GeneOntology No.
iron-cytochrome-c reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ferrocytochrome c + Fe3+ = ferricytochrome c + Fe2+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
ferrocytochrome-c:Fe3+ oxidoreductase
An iron protein.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-52-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ferrocytochrome c + Fe3+
ferricytochrome c + Fe2+
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ferrocytochrome c + Fe3+
ferricytochrome c + Fe2+
show the reaction diagram
-
close association of enzyme with respiration chain
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome
-
most highly purified fraction of the enzyme contains cytochrome c and apparently nonfunctional cytochrome b
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-oxo-3-phenylpropanoate
-
100% inhibition at 0.08 mM
Cr2+
-
slight inhibition
Cu2+
-
100% inhibition at 0.1 mM
cysteine
-
100% inhibition at 0.2 mM
Fe3+
-
48% inhibition at 1 mM
Ni2+
-
slight inhibition
p-hydroxymercuribenzoate
-
100% inhibition at 0.5 mM, inhibition can be reversed by reduced glutathione or cysteine
reduced glutathione
-
67% inhibition at 1 mM
additional information
-
not inhibitory: atebrin, amytal, antimycin A
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0275
cytochrome c
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6.7
-
pH 5: about 50% of maximum activity, pH 6.7: about 25% of maximum activity
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.5
-
several weeks, no loss of activity
22
-
8 h, no loss of activity
40
-
20 min, 10% loss of activity
60
-
5 min, 46% loss of activity
90
-
1 min, 74% loss of activity
100
-
1 min, complete loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0-5°C, several weeks without loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE