Information on EC 1.8.99.B2 - tetrathionate reductase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.8.99.B2
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
tetrathionate reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
tetrathionate + a reduced electron acceptor = 2 thiosulfate + an oxidized electron acceptor
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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A8FLS7
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 thiosulfate + ferricyande
tetrathionate + ferrocyanide
show the reaction diagram
tetrathionate + a reduced electron acceptor
2 thiosulfate + an oxidized electron acceptor
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
tetrathionate + a reduced electron acceptor
2 thiosulfate + an oxidized electron acceptor
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
heme
A8FLS7
sequence shows two Cys-X2-Cys-His heme c-binding motifs, bifunctional tetrathionate reductase/thiosulphate dehydrogenase
additional information
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absence of heme groups in the protein
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KCl
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200 mM, presence of 200 mM NaCl, 86% of the activity in presence of 200 mM NaCl
MgCl2
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200 mM, presence of 200 mM NaCl, 68% of the activity in presence of 200 mM NaCl
NaCl
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maximum activity in presence of 200 mM NaCl
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-heptyl-4-hydroxy-quinoline N-oxide
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1 microM, presence of 200 mM NaCl, 12% residual activity
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N-ethylmaleimide
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1 mM, presence of 200 mM NaCl, 84% residual activity
Sodium sulfate
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100 mM, presence of 200 mM NaCl, 57% residual activity
sodium sulfite
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0.1 mM, presence of 200 mM NaCl, 45% residual activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
tetrathionate
A8FLS7
pH 5.0, 42C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
426
tetrathionate
Campylobacter jejuni
A8FLS7
pH 5.0, 42C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
42600
tetrathionate
Campylobacter jejuni
A8FLS7
pH 5.0, 42C
2223
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64
A8FLS7
pH 5.0, 42C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
A8FLS7
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42
A8FLS7
assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36247
A8FLS7
x * 36247, calculated from sequence
99000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
71fold enrichment with a recovery of 4.3% of the total activity
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