Information on EC 1.8.98.3 - sulfite reductase (coenzyme F420)

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The expected taxonomic range for this enzyme is: Methanocaldococcus jannaschii

EC NUMBER
COMMENTARY hide
1.8.98.3
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RECOMMENDED NAME
GeneOntology No.
sulfite reductase (coenzyme F420)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrogen sulfide + 3 oxidized coenzyme F420 + 3 H2O = sulfite + 3 reduced coenzyme F420
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
hydrogen sulfide:coenzyme F420 oxidoreductase
The enzyme, isolated from the archaeon Methanocaldococcus jannaschii, is involved in sulfite detoxification and assimilation.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
sulfite + reduced coenzyme F420
hydrogen sulfide + oxidized coenzyme F420
show the reaction diagram
sulfite + reduced methyl viologen
hydrogen sulfide + oxidized methyl viologen
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sulfite + reduced coenzyme F420
hydrogen sulfide + oxidized coenzyme F420
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
siroheme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021
reduced coenzyme F420
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pH 7.0, temperature not specified in the publication
0.012
sulfite
-
pH 7.0, temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.09
-
pH 7.0, temperature not specified in the publication, substrate: sulfite and reduced methyl viologen
1.3
-
pH 7.0, temperature not specified in the publication, cell extract, substrate: sulfite and reduced coenzyme F420
18.2
-
pH 7.0, temperature not specified in the publication, purified enzyme, substrate: sulfite and reduced coenzyme F420
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70000
-
x * 70000, SDS-PAGE
350000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
activity in cell extracts is highly oxygen sensitive. About 92% activity is lost if the cell extract is exposed to air for 60 min. Dithiothreitol and 2-mercapotoethanol neither protect the enzyme from inactivation nor restore activity in an air-inactivated enzyme preparation
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674504
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
coenzyme F420-dependent sulfite reductase of Methanocaldococcus jannaschii, a sulfite-tolerant methanogen, is expressed with activity in Methanococcus maripaludis, a sulfite-sensitive methanogen. The recombinant organism reduces sulfite to sulfide and grows with sulfite as the sole sulfur source
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
sulfite induces coenzyme F420-dependent sulfite reductase