Information on EC 1.8.4.4 - glutathione-cystine transhydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.8.4.4
-
RECOMMENDED NAME
GeneOntology No.
glutathione-cystine transhydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 glutathione + cystine = glutathione disulfide + 2 cysteine
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
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reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Cysteine and methionine metabolism
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-
Glutathione metabolism
-
-
cysteine metabolism
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-
SYSTEMATIC NAME
IUBMB Comments
glutathione:cystine oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
37256-49-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-hydroxyethyl disulfide + GSH
?
show the reaction diagram
-
-
-
-
?
cystine + GSH
cysteine + GSSG
show the reaction diagram
D-cystine + 2 GSH
2 D-cysteine + GSSG
show the reaction diagram
-
-
-
-
?
diacetyl-L-cystine + GSH
?
show the reaction diagram
-
-
-
-
?
dimethyl disulfide + GSH
?
show the reaction diagram
-
-
-
-
?
dithiodiglycolic acid + GSH
?
show the reaction diagram
-
-
-
-
?
GSSG + GSH
GSSG + GSH
show the reaction diagram
-
-
-
-
?
homocystine + GSH
?
show the reaction diagram
-
-
-
-
?
L-cystine + GSH
L-cysteine + GSSG
show the reaction diagram
-
-
-
-
?
L-cystine diamide + GSH
?
show the reaction diagram
-
-
-
-
?
L-cystinyldiglycine + GSH
L-Cys-Gly-Gly + GSSG
show the reaction diagram
-
-
-
-
?
lipoic acid oxidized + GSH
?
show the reaction diagram
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-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cystine + GSH
cysteine + GSSG
show the reaction diagram
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.23
cystine
-
-
1.67
glutathione
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
61.6
-
beta-hydroxyethyldisulfide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.6
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pH 7.8 chosen for the assay to achieve smaller nonenzymatic rate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8.5
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pH 6.0: 10% of activity maximum, pH 8.5: activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
-
no activity in brush border
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
supernatant fraction
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
150000
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gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
24 h, complete inactivation
54
-
crude state, quite labile to heat above 54°C
60
-
denaturation, crude state
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, 1 M, complete stabilization, 6 days at 20°C
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cystine, alone: no stabilization, in addition to glutathione: increases stabilizing effect
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EDTA stabilizes
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ethylene glycol, 10%, stabilizes
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glutathione stabilizes, stabilizing effect increased by cystine
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glycerol, no stabilization
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, pH 5.8, 1 mM glutathione, several months
-
4°C, 24 h, complete inactivation, crude state
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE