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3'-phosphoadenosine-5'-phosphosulfate + thioredoxin
? + thioredoxin disulfide
substrate of phosphoadenylyl-sulfate reductase, EC 1.8.4.8
-
-
?
5'-adenylyl sulfate + glutathione
AMP + sulfite + glutathione disulfide
enzyme contains a C-terminal thioredoxin-like PDI domain, physiological electron acceptor unknown
-
?
5'-adenylyl sulfate + reduced dithiothreitol
AMP + sulfite + oxidized dithiothreitol
enzyme contains a C-terminal thioredoxin-like PDI domain, physiological electron acceptor unknown
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
adenosine 5'-phosphosulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
noncovalent complexes of the holoprotein with several ligands, including adenosine 5'-phosphosulfate, thioredoxin, and AMP, are investigated. AMP binds with a higher affinity to the enzyme intermediate than to the free enzyme
-
-
?
adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
additional information
?
-
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
-
ir
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
P71752
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
P71752
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
involved in assimilatory sulfate reduction
-
-
?
adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
-
?
adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
in Physcomitrella patens, APS reduction is not the major control step of sulfate assimilation
-
-
?
adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
sulfate assimilation pathway in Physcomitrella patens, overview
-
-
?
adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
dependent on thioredoxin, APS is the preferred substrate, substrate binding structure, modelling, overview
-
-
?
adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
-
-
-
?
additional information
?
-
-
importance of specific substituent groups for substrate binding
-
-
?
additional information
?
-
-
the moss Physcomitrella patens is unique among these organisms in possessing orthologs of both APR and PAPR, EC 1.8.4.8, genes
-
-
?
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5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
additional information
?
-
-
the moss Physcomitrella patens is unique among these organisms in possessing orthologs of both APR and PAPR, EC 1.8.4.8, genes
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
P71752
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
involved in assimilatory sulfate reduction
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
involved in assimilatory sulfate reduction
-
-
?
adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
in Physcomitrella patens, APS reduction is not the major control step of sulfate assimilation
-
-
?
adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
-
sulfate assimilation pathway in Physcomitrella patens, overview
-
-
?
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0.04 - 0.049
3'-phosphoadenosine-5'-phosphosulfate
0.0058 - 0.0243
5'-adenylyl sulfate
0.00175 - 0.004
adenosine-5'-phosphosulfate
3
glutathione
recombinant enzyme lacking transit peptide
0.4
reduced dithiothreitol
recombinant enzyme lacking transit peptide
-
0.04
3'-phosphoadenosine-5'-phosphosulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
0.049
3'-phosphoadenosine-5'-phosphosulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0058
5'-adenylyl sulfate
mutant D122G, pH not specified in the publication, temperature not specified in the publication
0.0069
5'-adenylyl sulfate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0109
5'-adenylyl sulfate
-
levulinate/rhodol-based assay, pH 7.5, 22°C
0.0114
5'-adenylyl sulfate
-
-
0.015
5'-adenylyl sulfate
-
levulinate/-hydroxy-4-methyl-2H-chromen-2-one-based assay, pH 7.5, 22°C
0.0243
5'-adenylyl sulfate
-
radioactive assay, pH 7.5, 22°C
0.00175
adenosine-5'-phosphosulfate
-
0.0025
adenosine-5'-phosphosulfate
recombinant enzyme lacking transit peptide, artificial reductand glutathione
0.003 - 0.004
adenosine-5'-phosphosulfate
-
0.0038
adenosine-5'-phosphosulfate
recombinant enzyme lacking transit peptide, artificial reductand dithiothreitol
0.01
thioredoxin
-
wild type enzyme, at 20°C in 10 mM phosphate buffer (pH 7.5) containing 100 mM Na2SO4
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Prior, A.; Uhrig, J.F.; Heins, L.; Wiesmann, A.; Lillig, C.H.; Stoltze, C.; Soll, J.; Schwenn, J.D.
Structural and kinetic properties of adenylyl sulfate reductase from Catharanthus roseus cell cultures
Biochim. Biophys. Acta
1430
25-38
1999
Pisum sativum, Catharanthus roseus (Q39619)
brenda
Bick, J.A.; Dennis, J.J.; Zylstra, G.J.; Nowack, J.; Leustek, T.
Identification of a new class of 5'-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria
J. Bacteriol.
182
135-142
2000
Mycobacterium sp., Pseudomonas putida, Ralstonia pickettii, Rhizobium tropici, Pseudomonas aeruginosa (O05927), Burkholderia cepacia (Q9RFS6), Burkholderia cepacia DBO1 / ATCC 29424 (Q9RFS6)
brenda
Abola, A.P.; Willits, M.G.; Wang, R.C.; Long, S.R.
Reduction of adenosine-5'-phosphosulfate instead of 3'-phosphoadenosine-5'-phosphosulfate in cysteine biosynthesis by Rhizobium meliloti and other members of the family Rhizobiaceae
J. Bacteriol.
181
5280-5287
1999
Agrobacterium tumefaciens, Sinorhizobium fredii, Rhizobium leguminosarum, Rhizobium sp., Sinorhizobium meliloti (P56891), Rhizobium sp. NGR234
brenda
Neumann, S.; Wynen, A.; Truper, H.G.; Dahl, C.
Characterization of the cys gene locus from Allochromatium vinosum indicates an unusual sulfate assimilation pathway
Mol. Biol. Rep.
27
27-33
2000
Allochromatium vinosum (Q9L9V0)
brenda
Williams, S.J.; Senaratne, R.H.; Mougous, J.D.; Riley, L.W.; Bertozzi, C.R.
5'-Adenosinephosphosulfate lies at a metabolic branch point in mycobacteria
J. Biol. Chem.
277
32606-32615
2002
Bacillus subtilis, Mycolicibacterium smegmatis, Mycobacterium tuberculosis (P71752)
brenda
Kim, S.K.; Gomes, V.; Gao, Y.; Chandramouli, K.; Johnson, M.K.; Knaff, D.B.; Leustek, T.
The two-domain structure of 5-adenylylsulfate (APS) reductase from Enteromorpha intestinalis is a requirement for efficient APS reductase activity
Biochemistry
46
591-601
2007
Pseudomonas aeruginosa
brenda
Kim, S.; Rahman, A.; Mason, J.T.; Hirasawa, M.; Conover, R.C.; Johnson, M.K.; Miginiac-Maslow, M.; Keryer, E.; Knaff, D.B.; Leustek, T.
The interaction of 5-adenylylsulfate reductase from Pseudomonas aeruginosa with its substrates
Biochim. Biophys. Acta
1710
103-112
2005
Pseudomonas aeruginosa
brenda
Lopez-Cortes, A.; Bursakov, S.; Figueiredo, A.; Thapper, A.E.; Todorovic, S.; Moura, J.J.; Ollivier, B.; Moura, I.; Fauque, G.
Purification and preliminary characterization of tetraheme cytochrome c3 and adenylylsulfate reductase from the peptidolytic sulfate-reducing bacterium Desulfovibrio aminophilus DSM 12254
Bioinorg. Chem. Appl.
3
81-91
2005
Desulfovibrio aminophilus
-
brenda
Gao, H.; Leary, J.; Carroll, K.S.; Bertozzi, C.R.; Chen, H.
Noncovalent Complexes of APS Reductase from M. tuberculosis: Delineating a Mechanistic Model Using ESI-FTICR MS
J. Am. Soc. Mass Spectrom.
18
167-178
2007
Mycobacterium tuberculosis
brenda
Martin, M.N.; Tarczynski, M.C.; Shen, B.; Leustek, T.
The role of 5-adenylylsulfate reductase in controlling sulfate reduction in plants
Photosynth. Res.
86
309-323
2005
Pseudomonas aeruginosa
brenda
Kopriva, S.; Fritzemeier, K.; Wiedemann, G.; Reski, R.
The putative moss 3'-phosphoadenosine-5'-phosphosulfate reductase is a novel form of adenosine-5-phosphosulfate reductase without an iron-sulfur cluster
J. Biol. Chem.
282
22930-22938
2007
Physcomitrium patens
brenda
Wiedemann, G.; Koprivova, A.; Schneider, M.; Herschbach, C.; Reski, R.; Kopriva, S.
The role of the novel adenosine 5-phosphosulfate reductase in regulation of sulfate assimilation of Physcomitrella patens
Plant Mol. Biol.
65
667-676
2007
Physcomitrium patens
brenda
Chung, J.S.; Noguera-Mazon, V.; Lancelin, J.M.; Kim, S.K.; Hirasawa, M.; Hologne, M.; Leustek, T.; Knaff, D.B.
Interaction domain on thioredoxin for Pseudomonas aeruginosa 5'-adenylylsulfate reductase
J. Biol. Chem.
284
31181-31189
2009
Pseudomonas aeruginosa
brenda
Hong, J.A.; Bhave, D.P.; Carroll, K.S.
Identification of critical ligand binding determinants in Mycobacterium tuberculosis adenosine-5'-phosphosulfate reductase
J. Med. Chem.
52
5485-5495
2009
Mycobacterium tuberculosis
brenda
Paritala, H.; Carroll, K.S.
A continuous spectrophotometric assay for adenosine 5-phosphosulfate reductase activity with sulfite-selective probes
Anal. Biochem.
440
32-39
2013
Mycobacterium tuberculosis
brenda
Stevenson, C.E.; Hughes, R.K.; McManus, M.T.; Lawson, D.M.; Kopriva, S.
The X-ray crystal structure of APR-B, an atypical adenosine 5-phosphosulfate reductase from Physcomitrella patens
FEBS Lett.
587
3626-3632
2013
Physcomitrium patens (Q8L5D0), Physcomitrium patens
brenda
Paritala, H.; Suzuki, Y.; Carroll, K.
Design, synthesis and evaluation of Fe-s targeted adenosine 5'-phosphosulfate reductase inhibitors
Nucleosides Nucleotides Nucleic Acids
34
199-220
2015
Mycobacterium tuberculosis
brenda