Information on EC 1.8.2.3 - sulfide-cytochrome-c reductase (flavocytochrome c)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.8.2.3
-
RECOMMENDED NAME
GeneOntology No.
sulfide-cytochrome-c reductase (flavocytochrome c)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrogen sulfide + 2 ferricytochrome c = sulfur + 2 ferrocytochrome c + 2 H+
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfide oxidation II (sulfide dehydrogenase)
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Sulfur metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
hydrogen-sulfide:flavocytochrome c oxidoreductase
The enzyme from Allochromatium vinosum contains covalently bound FAD and covalently-bound c-type hemes.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
hydrogen sulfide + ferricytochrome c 555
sulfur + ferrocytochrome c 555 + H+
show the reaction diagram
-
-
-
-
?
hydrogen sulfide + oxidized flavocytochrome c
sulfur + reduced flavocytochrome c + H+
show the reaction diagram
-
-
-
-
?
sulfide + oxidized benzyl viologen
sulfur + reduced benzyl viologen + H+
show the reaction diagram
sulfide + oxidized cytochrome c
sulfur + reduced cytochrome c + H+
show the reaction diagram
sulfide + oxidized flavocytochrome c
sulfur + reduced flavocytochrome c + H+
show the reaction diagram
sulfide + oxidized heart cytochrome c
sulfur + reduced heart cytochrome c + H+
show the reaction diagram
-
sulfur or polysulfide as product
-
?
sulfide + oxidized horse cytochrome c
sulfur + reduced horse cytochrome c + H+
show the reaction diagram
-
-
-
-
?
sulfide + oxidized Nitrosomonas europaea cytochrome c552
sulfur + reduced Nitrosomonas europaea cytochrome c552 + H+
show the reaction diagram
-
-
-
-
?
sulfide + oxidized Rhodospirillum rubrum cytochrome c2
sulfur + reduced Rhodospirillum rubrum cytochrome c2
show the reaction diagram
-
-
-
-
?
sulfur + reduced benzyl viologen + H+
sulfide + oxidized benzyl viologen
show the reaction diagram
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-
-
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
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the enzyme contains one molecule flavin
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Atebrin
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60% inhibition at 0.1 mM
cyanide
NaN3
-
7% inhibition at 10 mM
reduced heart cytochrome c
competitive product inhibition
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sulfite
preincubation of SoxF with 1 mM sodium sulfite for 20 min inactivates SoxF (9.0% residual sulfide dehydrogenase activity)
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
neither FAD nor FMN activate sulfide-cytochrome c reduction catalysed by cytochrome c-553
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0038
Oxidized cytochrome c
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pH and temperature not specified in the publication
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0.116
oxidized heart cytochrome c
at pH 6.0 and 30C
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0.0017 - 0.0125
Sulfide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.9
oxidized heart cytochrome c
Paracoccus pantotrophus
O07821
at pH 6.0 and 30C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0072
reduced heart cytochrome c
at pH 6.0 and 30C
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0.0013
sulfur
at pH 6.0 and 30C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0001
cyanide
Chlorobium vibrioforme f. thiosulfatophilum
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in 0.1 M Tris-HCl buffer, pH 7.4, at 20C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.1
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isoelectric focusing
6.7
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42010
calculated from amino acid sequence
58000
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gel filtration
61800
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calculated from amino acid sequence
64000
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gel filtration column reequilibrated with either intermediate or high ionic strength buffer
72000
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gel filtration
101000
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gel filtration column is reequilibrated with low ionic strength buffer
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
monomer
1 * 42797, electrospray mass spectrometry; 1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and DEAE-cellulose column chromatography
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ammonium sulfate precipitation and Q-Sepharose column chromatography
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Amberlite CG-50 column chromatography
DEAE-Sepharose column chromatography and Sephadex G-50 gel filtration
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muRPC C2/C18 PC 3.2/3 or muRPC C8 PC 2.1/10 column chromatography and Superdex 30 PC gel filtration
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in the absence of sulfide or thiosulfate, FCSD expression is significantly reduced
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the production of FCSD mRNA is upregulated by sulfide
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