Information on EC 1.8.2.3 - sulfide-cytochrome-c reductase (flavocytochrome c)

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.8.2.3
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RECOMMENDED NAME
GeneOntology No.
sulfide-cytochrome-c reductase (flavocytochrome c)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrogen sulfide + 2 ferricytochrome c = sulfur + 2 ferrocytochrome c + 2 H+
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
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sulfide oxidation II (sulfide dehydrogenase)
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Sulfur metabolism
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SYSTEMATIC NAME
IUBMB Comments
hydrogen-sulfide:flavocytochrome c oxidoreductase
The enzyme from Allochromatium vinosum contains covalently bound FAD and covalently-bound c-type hemes.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
hydrogen sulfide + ferricytochrome c 555
sulfur + ferrocytochrome c 555 + H+
show the reaction diagram
-
-
-
-
?
hydrogen sulfide + oxidized flavocytochrome c
sulfur + reduced flavocytochrome c + H+
show the reaction diagram
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-
-
-
?
sulfide + oxidized benzyl viologen
sulfur + reduced benzyl viologen + H+
show the reaction diagram
sulfide + oxidized cytochrome c
sulfur + reduced cytochrome c + H+
show the reaction diagram
sulfide + oxidized flavocytochrome c
sulfur + reduced flavocytochrome c + H+
show the reaction diagram
sulfide + oxidized heart cytochrome c
sulfur + reduced heart cytochrome c + H+
show the reaction diagram
-
-
sulfur or polysulfide as product
-
?
sulfide + oxidized horse cytochrome c
sulfur + reduced horse cytochrome c + H+
show the reaction diagram
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-
-
-
?
sulfide + oxidized Nitrosomonas europaea cytochrome c552
sulfur + reduced Nitrosomonas europaea cytochrome c552 + H+
show the reaction diagram
-
-
-
-
?
sulfide + oxidized Rhodospirillum rubrum cytochrome c2
sulfur + reduced Rhodospirillum rubrum cytochrome c2
show the reaction diagram
-
-
-
-
?
sulfur + reduced benzyl viologen + H+
sulfide + oxidized benzyl viologen
show the reaction diagram
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-
-
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
flavin
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the enzyme contains one molecule flavin
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Atebrin
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60% inhibition at 0.1 mM
cyanide
NaN3
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7% inhibition at 10 mM
reduced heart cytochrome c
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competitive product inhibition
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sulfite
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preincubation of SoxF with 1 mM sodium sulfite for 20 min inactivates SoxF (9.0% residual sulfide dehydrogenase activity)
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
neither FAD nor FMN activate sulfide-cytochrome c reduction catalysed by cytochrome c-553
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0038
Oxidized cytochrome c
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pH and temperature not specified in the publication
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0.116
oxidized heart cytochrome c
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at pH 6.0 and 30°C
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0.0017 - 0.0125
Sulfide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.9
oxidized heart cytochrome c
Paracoccus pantotrophus
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at pH 6.0 and 30°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0072
reduced heart cytochrome c
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at pH 6.0 and 30°C
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0.0013
sulfur
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at pH 6.0 and 30°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0001
cyanide
Chlorobium vibrioforme f. thiosulfatophilum
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in 0.1 M Tris-HCl buffer, pH 7.4, at 20°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.1
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isoelectric focusing
6.7
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
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1 * 46000 + 1 * 10000, SDS-PAGE
21300
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1 * 46600 + 1 * 21300, SDS-PAGE
40000
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1 * 40000 + 1 * 11000, SDS-PAGE
42010
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calculated from amino acid sequence
42797
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1 * 42797, electrospray mass spectrometry
42832
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1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da
43568
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1 * 43568 + 1 * ?, native flavoprotein subunit, calculated from amino acid sequence (without disulfide bridges but with FAD)
43652
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1 * 43652 + 1 * ?, native flavoprotein subunit, MALDI-TOF mass spectrometry
46600
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1 * 46600 + 1 * 21300, SDS-PAGE
47000
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1 * 47000 + 1 * 11000, flavoprotein subunit and cytochrome subunit, SDS-PAGE
58000
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gel filtration
61800
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calculated from amino acid sequence
64000
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gel filtration column reequilibrated with either intermediate or high ionic strength buffer
72000
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gel filtration
101000
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gel filtration column is reequilibrated with low ionic strength buffer
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
monomer
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1 * 42797, electrospray mass spectrometry; 1 * 42832, electrospray mass spectrometry of SoxF as isolated yields masses of 42797 Da and 42832 Da
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation and DEAE-cellulose column chromatography
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ammonium sulfate precipitation and Q-Sepharose column chromatography
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ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Amberlite CG-50 column chromatography
DEAE-Sepharose column chromatography and Sephadex G-50 gel filtration
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muRPC C2/C18 PC 3.2/3 or muRPC C8 PC 2.1/10 column chromatography and Superdex 30 PC gel filtration
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in the absence of sulfide or thiosulfate, FCSD expression is significantly reduced
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the production of FCSD mRNA is upregulated by sulfide
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