Information on EC 1.8.1.13 - bis-gamma-glutamylcystine reductase

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The expected taxonomic range for this enzyme is: Halobacterium salinarum

EC NUMBER
COMMENTARY hide
1.8.1.13
-
RECOMMENDED NAME
GeneOntology No.
bis-gamma-glutamylcystine reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 gamma-glutamylcysteine + NADP+ = bis-gamma-glutamylcystine + NADPH + H+
show the reaction diagram
highly specific; highly specific. Not identical with EC 1.8.1.7 glutathione-disulfide reductase or EC 1.8.1.14 CoA-disulfide reductase
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glutathione metabolism
-
-
Glutathione metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
gamma-glutamylcysteine:NADP+ oxidoreductase
Contains FAD. The enzyme, which is found only in halobacteria, maintains the concentration of gamma-glutamylcysteine, the major low molecular weight thiol in halobacteria. Not identical with EC 1.8.1.7 (glutathione-disulfide reductase) or EC 1.8.1.14 (CoA-disulfide reductase).
CAS REGISTRY NUMBER
COMMENTARY hide
117056-54-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
enzyme is a member of the pyridine nucleotide disulfide oxidoreductase family
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
4-nitrobenzenethiol + NADP+
show the reaction diagram
-
-
-
-
?
bis-gamma-glutamylcystine + NADPH + H+
2 gamma-glutamylcysteine + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
bis-gamma-glutamylcystine + NADPH + H+
2 gamma-glutamylcysteine + NADP+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
less effective than NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AsO2-
-
25 mM arsenite, relative activity 46%
Cu2+
-
relative activity less than 5%
Hg2+
-
relative activity less than 5%
Zn2+
-
relative activity less than 44%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.81 - 1.1
bis-gamma-glutamylcystine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
54
bis-gamma-glutamylcystine
Halobacterium salinarum
Q9HSN0
pH 6.7, temperature not specified in the publication
28.3
FAD
Halobacterium salinarum
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
49
bis-gamma-glutamylcystine
Halobacterium salinarum
Q9HSN0
pH 6.7, temperature not specified in the publication
15665
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3 - 8.2
-
about half-maximal activity at pH 6.3 and 8.2
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
122000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 61000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
retains full activity after incubation in high-and intermediate-ionic-strength buffers, inactivated after incubation in low-ionic strength buffers
80
-
inactivation in buffers of each of the 3 ionic strengths, low-ionic, intermediate-ionic and high-ionic
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
high-ionic-strength buffer above 2 M stabilizes during purification and storage
-
high-ionic-strength buffer above 3.5 M increases thermal stability
-
low-ionic-strength buffer below 1.0 M leads to rapid denaturation
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
20°C, stable to prolonged storage at room temperature in high-ionic-strength buffer above 3.5 M
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
both native enzyme and recombinant enzyme, from inclusion bodies
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE