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Information on EC 1.8.1.13 - bis-gamma-glutamylcystine reductase Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Halobacterium salinarum
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bis-gamma-glutamylcystine reductase
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2 gamma-glutamylcysteine + NADP+ = bis-gamma-glutamylcystine + NADPH + H+
highly specific; highly specific. Not identical with EC 1.8.1.7 glutathione-disulfide reductase or EC 1.8.1.14 CoA-disulfide reductase
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glutathione metabolism
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Glutathione metabolism
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gamma-glutamylcysteine:NADP+ oxidoreductase
Contains FAD. The enzyme, which is found only in halobacteria, maintains the concentration of gamma-glutamylcysteine, the major low molecular weight thiol in halobacteria. Not identical with EC 1.8.1.7 (glutathione-disulfide reductase) or EC 1.8.1.14 (CoA-disulfide reductase).
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EC 1.6.4.9
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formerly
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NADPH2:bis-g-glutamylcysteine oxidoreductase
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GCR
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brenda
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UniProt
brenda
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physiological function
enzyme is a member of the pyridine nucleotide disulfide oxidoreductase family
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5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
4-nitrobenzenethiol + NADP+
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bis-gamma-glutamylcystine + NADPH + H+
2 gamma-glutamylcysteine + NADP+
additional information
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bis-gamma-glutamylcystine + NADPH + H+
2 gamma-glutamylcysteine + NADP+
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bis-gamma-glutamylcystine + NADPH + H+
2 gamma-glutamylcysteine + NADP+
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additional information
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no activity detected with GSSG
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additional information
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no activity detected with GSSG
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lipoic acid disulfide and dihydrolipoamide are no substrates
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lipoic acid disulfide and dihydrolipoamide are no substrates
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enzyme is annotated as mercuric reductase. Enzyme that is expressed in Escherichia coli and refolded from inclusion bodies has robust bis-gamma-glutamylcystine reductase activity but no mercuric reductase activity
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bis-gamma-glutamylcystine + NADPH + H+
2 gamma-glutamylcysteine + NADP+
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NADH
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less effective than NADPH
FAD
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FAD
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flavoprotein, 1 mol FAD/mol subunit
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AsO2-
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25 mM arsenite, relative activity 46%
Cu2+
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relative activity less than 5%
Hg2+
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relative activity less than 5%
Zn2+
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relative activity less than 44%
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0.81 - 1.1
bis-gamma-glutamylcystine
0.81
bis-gamma-glutamylcystine
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1.1
bis-gamma-glutamylcystine
pH 6.7, temperature not specified in the publication
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54
bis-gamma-glutamylcystine
pH 6.7, temperature not specified in the publication
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49
bis-gamma-glutamylcystine
pH 6.7, temperature not specified in the publication
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6.3 - 8.2
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about half-maximal activity at pH 6.3 and 8.2
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brenda
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61000
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2 * 61000, SDS-PAGE
122000
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gel filtration
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dimer
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2 * 61000, SDS-PAGE
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retains full activity after incubation in high-and intermediate-ionic-strength buffers, inactivated after incubation in low-ionic strength buffers
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inactivation in buffers of each of the 3 ionic strengths, low-ionic, intermediate-ionic and high-ionic
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high-ionic-strength buffer above 2 M stabilizes during purification and storage
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high-ionic-strength buffer above 3.5 M increases thermal stability
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low-ionic-strength buffer below 1.0 M leads to rapid denaturation
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20°C, stable to prolonged storage at room temperature in high-ionic-strength buffer above 3.5 M
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both native enzyme and recombinant enzyme, from inclusion bodies
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Q18DN0_HALWD
Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)
468
50604
TrEMBL
G0LG64_HALWC
Haloquadratum walsbyi (strain DSM 16854 / JCM 12705 / C23)
468
50645
TrEMBL
A0A0U5H270_9EURY
461
50216
TrEMBL
B0R2S2_HALS3
Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)
465
50261
TrEMBL
Q9HSN0_HALSA
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
465
50261
TrEMBL
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Sundquist, A.R.; Fahey, R.C.
The novel disulfide reductase bis-gamma-glutamylcystine reductase and dihydrolipoamide dehydrogenase from Halobacterium halobium: Purification by immobilized-metal-ion affinity chromatography and properties of the enzymes
J. Bacteriol.
170
3459-3467
1988
Halobacterium salinarum
brenda
Sundquist, A.R.; Fahey, R.C.
The function of gamma-glutamylcysteine and bis-gamma-glutamylcystine reductase in Halobacterium halobium
J. Biol. Chem.
264
719-725
1989
Halobacterium salinarum
brenda
Kim, J.; Copley, S.D.
The orphan protein bis-?-glutamylcystine reductase joins the pyridine nucleotide disulfide reductase family
Biochemistry
52
2905-2913
2013
Halobacterium salinarum (Q9HSN0)
brenda
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