Information on EC 1.8.1.10 - CoA-glutathione reductase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.8.1.10
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RECOMMENDED NAME
GeneOntology No.
CoA-glutathione reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CoA + glutathione + NADP+ = CoA-glutathione + NADPH + H+
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
coenzyme A transfer
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oxidation
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redox reaction
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-
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Cysteine and methionine metabolism
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SYSTEMATIC NAME
IUBMB Comments
glutathione:NADP+ oxidoreductase (CoA-acylating)
A flavoprotein. The substrate is a mixed disulfide. May be identical to EC 1.8.1.9, thioredoxin-disulfide reductase.
CAS REGISTRY NUMBER
COMMENTARY hide
37256-33-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
strain ATCC 1946
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Manually annotated by BRENDA team
strain 8325-4
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Manually annotated by BRENDA team
strain 8325-4
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CoA disulfide + NADPH
CoA + NADP+
show the reaction diagram
CoA-glutathione + NADPH
glutathione + CoA + NADP+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CoA disulfide + NADPH
CoA + NADP+
show the reaction diagram
CoA-glutathione + NADPH
glutathione + CoA + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
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flavoprotein, gene with two FAD-binding regions
NADPH
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphate
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25 mM, partially inhibits
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.014
CoA disulfide
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recombinant enzyme expressed in E. coli
0.2 - 0.23
CoA-glutathione
0.0019
NADPH
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recombinant enzyme expressed in E. coli
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1100
CoA disulfide
Staphylococcus aureus
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0007
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alloxan diabetic rats, liver extract
0.00085
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normal rats, liver extract
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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assay at
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42500
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gel filtration, one protein with CoASSG reductase and GSSG reductase activities, EC 1.6.4.2
49200
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2 * 49200, calculated from the nucleotide sequence
90000
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native molecular weight
108000
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sucrose density gradient ultracentrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, dialyzed extract, 16 hours, stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
143fold partial purification, separated from GSSG reductase activity, EC 1.6.4.2
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289fold purification; not separated from GSSG reductase activity, EC 1.6.4.2
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not separated from GSSG reductase activity, EC 1.6.4.2
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purification of recombinant CoADR, expressed in Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cdr gene encoding coenzyme A glutathione disulfide reductase is cloned, sequenced and overexpressed in Escherichia coli BL21 using the pET expression system, gene encodes a 438-amino acid polypeptide
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
pharmacology