Information on EC 1.7.5.2 - nitric oxide reductase (menaquinol)

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The expected taxonomic range for this enzyme is: Bacillus azotoformans

EC NUMBER
COMMENTARY hide
1.7.5.2
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RECOMMENDED NAME
GeneOntology No.
nitric oxide reductase (menaquinol)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 nitric oxide + menaquinol = nitrous oxide + menaquinone + H2O
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
nitrate reduction VII (denitrification)
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-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nitric oxide + ascorbic acid
?
show the reaction diagram
nitric oxide + cytochrome c551
?
show the reaction diagram
nitric oxide + juglone
?
show the reaction diagram
-
-
-
-
?
nitric oxide + menaquinol
nitrous oxide + menaquinone + H2O
show the reaction diagram
nitric oxide + plumbagin
?
show the reaction diagram
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-
-
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
copper
-
the enzyme contains a CuA center in its small subunit
Iron
-
the enzyme contains one non-heme iron per enzyme complex. The non-heme iron content is 13.7 nmol/mg
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome c551
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noncompetitive inhibitor of nitric oxide reduction when menaquinol is used as an electron donor
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ferricytochrome c
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NOR activity is stimulated by ferrocytochrome c when phenazine methosulfate and ascorbate are both present
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0045
cytochrome c551
-
pH and temperature not specified in the publication
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0.141
juglone
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pH and temperature not specified in the publication
0.256
Plumbagin
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pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
41 - 153
nitric oxide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.67
-
crude membrane extract, pH and temperature not specified in the publication
40.7
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after 60.5fold purification, pH and temperature not specified in the publication
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16000
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1 * 16000 + 1 * 40000, SDS-PAGE
40000
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1 * 16000 + 1 * 40000, SDS-PAGE
112000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Q-Sepharose column chromatography, Sephadex G25 gel filtration, and Bio-Scale Ceramic CHT20-I hydroxyapatite column chromatography
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